Recombinant protein for detecting toxoplasma gondii and encoding gene of recombinant protein

A gene and coding technology is applied in the field of detection of recombinant protein of Toxoplasma gondii and its coding gene, which can solve the problems of poor specificity and low sensitivity of detection of Toxoplasma gondii, and achieves improved detection specificity and sensitivity, clear components and low cost. Effect
CN111116726APending Publication Date: 2020-05-08INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Publication Date
2020-05-08

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Abstract

The embodiment of the invention discloses a protein which is a protein of the following a) or b): a) a protein consisting of amino acid sequences shown in SEQ ID NO:2, and b) a protein which consistsof amino acid sequences that amino acid sequences shown in SEQ ID NO:2 are substituted and / or deleted and / or added by one or more amino acid residues, and is specifically combined with antiserum of the toxoplasma gondii. According to the embodiment of the invention, genes of MIC2, MIC3 and SAG1 of the toxoplasma gondii are subjected to antigenicity analysis, a high antigenicity area is cut, codonoptimization is implemented according to preference of escherichia coli codon, a Linker (of which the amino acid sequence is GGGGSGGGGSGGGGS) is added for serial connection, a recombinant expression vector and a recombinant expression bacterium are constructed, MIC2-MIC3-SAG1 recombinant expression proteins of the toxoplasma gondii are obtained, and meanwhile, the defect that the yield of the expression protein is low is overcome.
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Description

technical field

[0001] The embodiment of the present invention relates to the field of biotechnology, in particular to a recombinant protein and its coding gene for detecting Toxoplasma gondii. Background technique

[0002] Toxoplasmosis is a zoonotic parasitic disease caused by Toxoplasma gondii infection that can cause abortion, deformity or stillbirth in humans and animals. It is prevalent worldwide and seriously endangers public health and safety. Livestock production. Pregnant women, cancer patients, AIDS patients and other infections are particularly serious. Pregnant women infected with toxoplasmosis can lead to miscarriage, stillbirth, fetal malformation, and even fetal growth retardation and mental retardation. In addition, organ transplant patients and AIDS patients and other immunocompromised patients infected with Toxoplasma gondii can lead to death.

[0003] Dogs and cats play an important role in the life history of Toxoplasma gondii. As the final host of Tox...

Claims

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