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Alcaligenes faecalis as well as application in degradation of ethylene oxide

A technology of Alcaligenes faecalis and ethylene oxide is applied in the field of microorganisms to achieve the effect of eliminating hazards

Inactive Publication Date: 2020-05-08
CHINESE RES ACAD OF ENVIRONMENTAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Microbial degradation of harmful substances has become an important part of the chemical industry. However, there are few effective strains used to degrade ethylene oxide at present. It is necessary to effectively screen out microorganisms that degrade ethylene oxide in order to improve the efficiency of ethylene oxide. Alkanes harmless treatment capacity

Method used

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  • Alcaligenes faecalis as well as application in degradation of ethylene oxide
  • Alcaligenes faecalis as well as application in degradation of ethylene oxide
  • Alcaligenes faecalis as well as application in degradation of ethylene oxide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Screening of ethylene oxide degrading potential bacteria

[0021] The sample in this example is taken from the sludge mixture at the outfall of the sewage treatment plant in the suburbs of Guangzhou, Guangdong Province:

[0022] 1) Weigh 10.0 g of the sample sludge mixture, add 100 mL of 0.03 mol / L phosphate buffer solution to it, mix and clarify for 120 min, filter to remove large particles of sediment, and obtain a suspension;

[0023] 2) Take 1 mL of the suspension and add it to 4 test tubes containing 10 mL of liquid enrichment medium (tryptone soybean broth medium containing 100 mg / L ethylene oxide), and place it on a shaker for oxygen enrichment. Collect culture, rotate at 200r / min, culture at 37°C for 24-48h;

[0024] 3) Streak and separate the dominant strains in the liquid enrichment culture medium on the screening medium, and isolate the ethylene oxide degrading potential bacteria. A single colony was picked and cultured in an enrichment liquid med...

Embodiment 2

[0028] Example 2 identification

[0029] The identification method of bacteria species is:

[0030] a. Identification of morphological characteristics: observation of colony morphology, microscopic observation of bacteria, culture characteristics and identification of Gram staining;

[0031] b. Physiological and biochemical characteristics identification: physical and chemical characteristics include nutrient type, nitrogen and carbon source utilization ability and biochemical tests, etc.;

[0032] c. Molecular biological identification: Bacterial culture, bacterial DNA extraction, PCR amplification, 16s gene sequencing and sequence comparison analysis are carried out in sequence;

[0033] Upstream primer 27F: 5'-AGAGTTTGATCCTGGCTCAG-3', as shown in SEQ ID NO.1;

[0034] Downstream primer 1492R: 5'-GGTTACCTTGTTACGACTT-3', as shown in SEQ ID NO.2;

[0035] The gene sequencing result is shown in SEQ ID NO.3; the 16S rRNA sequence was compared with the nucleotide sequence BLAS...

Embodiment 3

[0038] Embodiment 3 induces domestication

[0039] Stage 1: Ethylene oxide tolerance induction acclimation: Inoculate the ethylene oxide degrading potential bacteria in the tolerance acclimation medium containing 100 mg / L ethylene oxide by using the plate streak method, and culture at 37°C for 48 hours ;Pick the single colony with the largest colony radius on the plate, subculture to the tolerance acclimation medium containing 200mg / L ethylene oxide, and cultivate at a constant temperature of 37°C for 48h; select the single colony with the largest colony radius on the plate, and subculture to the medium containing In 500mg / L ethylene oxide tolerance acclimation medium, culture at 37°C for 48 hours; select the single colony with the largest colony radius on the plate, subculture to 800mg / L ethylene oxide tolerance acclimatization medium, 37°C Cultivate at constant temperature for 48 hours, select the single colony with the largest colony radius on the plate, and obtain a high-c...

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Abstract

The invention discloses Alcaligenes faecalis as well as application thereof. The strain is collected in CGMCC on August 29, 2019 with the collection number of CGMCC No.18435 in the Institute of Microbiology of the Chinese academy of Science No.3 No.1 courtyard in Beichen West Road in the Chaoyang District of Beijing; and the taxonomic name of the strain is Alcaligenes faecalis, named EO-05. The strain is applied to industrial degradation of ethylene oxide, and improves the harmless treatment capacity for the ethylene oxide.

Description

technical field [0001] The invention relates to the technical field of microbes, more specifically to Alcaligenes faecalis and its application in degrading ethylene oxide. Background technique [0002] Ethylene oxide is one of the important petrochemical products in the modern chemical industry. Due to its high penetrability and ability to couple with biomacromolecules, ethylene oxide plays an important role in the medical sterilization industry. In addition, the cost of ethylene oxide sterilization is low, and it can be used for industrial-scale large-scale sterilization. It is one of the most important low-temperature sterilizers so far. There is a large amount of ethylene oxide remaining in petrochemical industrial sewage, but because ethylene oxide is extremely active, flammable and explosive, and is also a world-recognized carcinogen, it needs to be degraded. [0003] At present, the harmless treatment method of ethylene oxide in my country is mainly to use sulfuric a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/36C02F3/34C12R1/05C02F101/34
CPCC02F3/34C02F2101/34C12N1/36C12N1/205C12R2001/05
Inventor 刘征涛薛建龙侯东新王晓南王石虎谭金水尹昕曾育平
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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