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Strip electrode, strip electrode based vegf 165 Sensor and preparation method and detection method thereof

A sensor and strip electrode technology, applied in the field of VEGF165 sensor and its preparation, can solve the problems of expensive, time-consuming, and laborious, and achieve the effects of low cost, simple preparation, and non-immunogenicity

Active Publication Date: 2022-07-12
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, several traditional methods for the detection of VEGF have been reported in the literature, however, these techniques are costly, laborious and time-consuming due to their expensive instruments and complicated sample pretreatment

Method used

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  • Strip electrode, strip electrode based vegf <sub>165</sub> Sensor and preparation method and detection method thereof
  • Strip electrode, strip electrode based vegf <sub>165</sub> Sensor and preparation method and detection method thereof
  • Strip electrode, strip electrode based vegf <sub>165</sub> Sensor and preparation method and detection method thereof

Examples

Experimental program
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Embodiment 1

[0031] Example 1. Making strip electrodes

[0032] The strip and strip electrode (LPLE) of the present invention is made of FR-4 epoxy glass cloth as a base material, and is made by immersing gold on a printed circuit board. The electrode is a kind of capacitive electrode, which is mainly composed of two parts of capacitance, namely, the bar-to-block capacitance and the block-to-block capacitance.

[0033] The strip electrode of the present invention is structured as follows figure 1 Shown: it is mainly composed of a substrate 1 and electrodes, the electrodes are capacitor electrodes, the input electrodes are two opposite strip electrodes 2, and a plurality of block electrodes 3 are evenly distributed between the two strip electrodes 2. The electrodes are made by immersing gold on the substrate 1, one of the strip electrodes 2 is T-shaped, the other strip electrode 2 is U-shaped, and the T-shaped strip electrodes 2 include horizontal bars 2a and vertical bars. 2b, the top en...

Embodiment 2

[0036] Example 2. Detection and application of vascular endothelial cell growth factor 165

[0037] The strip electrode of Example 1 was used to make the detection of VEGF 165 The impedance sensor (the sensor works as Figure 5 shown), follow the steps below:

[0038] Step 1. Treatment of strip electrodes

[0039] Take the strip electrodes made in Example 1 and clean them successively: 1M sodium hydroxide for 5min, 1M hydrochloric acid for 3min, NH 3 :H 2 O 2 :H 2 O (1:1:5, v / v / v) mixed solution was cleaned for 2 min, then rinsed with ultrapure water (18.25 MΩ·cm), and dried with nitrogen for use.

[0040] Step 2. Preparation of aptamers

[0041] A thiol-modified aptamer (5'-SH-TTTCCCGTCTTCCAGACAAGAGGTGCAG-GG-3') was selected, and 1 μM of the aptamer solution was reacted with 10 mM TCEP for 1 h at room temperature to activate DNA and open disulfide bonds. The aptamer solution was drop-coated on the cleaned strip electrode surface, incubated at 4°C for 24 h, and the apt...

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Abstract

The invention discloses a bar-shaped bar electrode, which comprises two opposite bar-shaped electrodes, and a plurality of bar-shaped electrodes are distributed between the two bar-shaped electrodes. Also disclosed is a kind of VEGF based on strip electrodes 165 Sensors including the aforementioned strip electrodes with VEGF immobilized on the electrodes of the strip electrodes 165 aptamer. A preparation method of the sensor is also disclosed: 1) Take strip electrodes, clean and dry; 2) VEGF 165 The aptamer solution was dripped onto the surface of the strip electrodes and incubated. The detection method of the sensor is also disclosed: taking the aforementioned VEGF based on strip electrodes 165 Sensor, drip the solution to be tested on the surface of the electrode, incubate, and then connect with an electrochemical analyzer or impedance measuring instrument to measure its impedance value at different frequencies, and determine whether the solution to be tested contains VEGF through the corresponding impedance diagram calculation 165 and containing VEGF 165 concentration.

Description

technical field [0001] The invention belongs to the technical field of electrochemical sensors, and in particular relates to a strip electrode and a VEGF based strip electrode 165 A sensor and its preparation method and detection method. Background technique [0002] Malignant tumor has a high mortality rate and is a major disease that threatens human life and health. Due to its invasiveness and destructiveness in the human body, the rapid growth of malignant tumors can destroy the tissue structure of human organs and cause dysfunction. According to the "Analysis of Incidence and Death of Malignant Tumors in China in 2015" released by the National Cancer Center in 2019, there were 3.929 million new cases of malignant tumors in my country in 2015, with an incidence rate of 2.8583 / 100,000, 2.338 million deaths and 2.338 million deaths. Rate 170.05 / 100,000. Malignant tumors have become the first cause of death among Chinese residents, and lung cancer, liver cancer, gastric ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/416
CPCG01N27/30G01N27/3275G01N27/416
Inventor 陈龙聪冯英刘改琴熊兴良王洪雷高斌江奇锋孔凡凯
Owner CHONGQING MEDICAL UNIVERSITY