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Bacillus acid fast growing bacillus syy15 and its application

A bacillus and sprouting technology, which is applied to Bacillus acid fast-growing Bacillus SYY15 and its application field to achieve the effect of improving plant disease resistance, obvious effect and enhancing stress resistance

Active Publication Date: 2022-04-01
GENLIDUO BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on plant stress-resistant growth-promoting and saline-alkali land restoration. The present invention provides a strain of Bacillus acid fast-growing SYY15 strain that not only has the function of promoting growth, but also can improve the tolerance of crops to external stress, improve crop resistance

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  • Bacillus acid fast growing bacillus syy15 and its application
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  • Bacillus acid fast growing bacillus syy15 and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1 Isolation and Identification of Bacterial Strain SYY15

[0030] Soil was collected from the experimental demonstration base of Genliduo Biotechnology Co., Ltd. in Weixian County, Hebei Province, and 10 g of soil samples were added to a triangular flask filled with 100 ml of sterile saline. Add 1ml sample to 9ml sterile normal saline, and then dilute 10 2 , 10 3 , 10 4 Take 100 μl of the above soil suspension and spread it evenly on the plate of LB medium (yeast powder 5g / L, peptone 10g / L, NaCl 10g / L, agar 15g / L), and culture it in the incubator at 28°C for 48h. Pick up a single bacterial colony with a sterile toothpick, streak and purify it, and save it for later use.

[0031] Based on the physiological and biochemical characteristics of the strain SYY15 and the results of molecular biology tests, it was identified as Bacillus acidiceler. The specific identification results are as follows:

[0032] 1. Molecular biological identification

[0033] Firstly,...

Embodiment 2

[0040] Example 2 Ability of bacterial strain SYY15 to produce ACC deaminase

[0041] The strain SYY15 was first activated on LB medium. The activated strain was inoculated into DFa medium (KH 2 PO 4 4g, Na 2 HPO 4 6g, MgSO 4 ·7H 2 O 0.2g, glucose 2g, gluconic acid 2g, citric acid 2g, ACC final concentration of 3mM, water 1000ml, pH 7.2), 30°C, 180r / min under shaking table culture for 24h, with the culture solution of no strain As a control, detect the absorbance value of the culture solution at 600nm, and judge whether the strain can grow in the culture solution with ACC as the only nitrogen source according to the absorbance value. If it can grow, it is transferred to DFa culture medium and cultured continuously for 3 times. The results showed that the strain SYY15 could grow in the culture medium with ACC as the sole nitrogen source, OD 600 can reach 2.35 ( figure 1 ).

Embodiment 3

[0042] Example 3 Determination of Phosphorus Solubilizing Ability of Bacterial Strain SYY15

[0043] The strains were first activated on LB medium. Inoculate the activated bacterial strain into the phosphate-dissolving medium shown in Appendix A of NY / T 1847-2010 (glucose 10.00g, (NH 4 ) 2 SO 4 0.50g, MnSO 4 ·7H 2 O 0.3g, NaCl 0.3g, KCl 0.30g, FeSO 4 4H 2 O 0.036g, MnSO 4 4H 2 O 0.03g, distilled water 1000mL, pH 7.0. The organic phosphorus source is calcium phytate, and the addition amount is 2g. Solid medium: add 1.5% agar powder in proportion). Detect whether it has the function of dissolving phosphorus. After testing, the bacterial strain SYY15 has the ability to dissolve organic phosphorus, and the diameter of the dissolution circle for dissolving organic phosphorus reaches 1.34cm ( figure 2 ).

[0044] After confirming that the strain SYY15 has the phosphorus-dissolving function, the phosphorus-dissolving ability of the strain was quantitatively measured. Af...

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Abstract

The invention discloses Bacillus acid fast-growing SYY15 and its application. The preservation number of strain SYY15 is CCTCC NO: M 2019840. The strain can not only synthesize ACC deaminase, enhance the stress resistance of crops, but also degrade insoluble organic phosphorus and inorganic phosphorus in large quantities, and produce IAA, protease and other biomass-promoting substances . Taking maize as an example, strain SYY15 has obvious effect on promoting plant stress resistance. Compared with the salt stress group, the seed germination coefficient of the strain SYY15 treatment group could be increased by 34.10%. The application of strain SYY15 in crop planting can not only improve crop yield, but also improve plant resistance to disease, drought and other stress resistance, which is of great significance to the continuous increase of grain production in my country and the protection of farmland ecological environment.

Description

technical field [0001] The invention relates to agricultural microbiology, in particular to a strain of acid fast-growing bacillus SYY15 and its application. Background technique [0002] Stress is defined as a factor that negatively affects plant growth, and any form of stress increases the formation of reactive oxygen species (ROS), such as H2O2, O2-, and OH-. Excess reactive oxygen species easily cause oxidative stress, which damages plants by oxidizing photosynthetic pigments, delipids, proteins and nucleic acids. Abiotic stresses (high winds, extreme temperatures, drought, salinization, flooding, etc.) have a negative impact of up to 70% on biomass production and the yield of staple food crops, threatening global food security. Plants are often subjected to various environmental stresses and have developed specific response mechanisms. In the past few decades, through various researches, people have understood the mechanisms of abiotic and biotic stress resistance. It...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/54C12N9/78C12P17/10C05G3/00C05G3/40A01N63/22A01P21/00C09K17/14C12R1/07C09K101/00
CPCC12N1/20C12N9/54C12N9/78C12Y305/99007C12P17/10C05G3/00C09K17/14C09K2101/00C12R2001/07C12N1/205
Inventor 邢明振王秀艳张云鸽郭岩彬柴彦
Owner GENLIDUO BIO TECH CO LTD
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