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Collagen peptide used for intestinal absorption, and extraction method and application thereof

A collagen peptide, intestinal absorption technology, applied in the preparation method, application, peptide/protein composition of peptides, etc., can solve the problem of few deep-processed products, achieve obvious anti-aging, obvious effect, and reduce production costs.

Pending Publication Date: 2020-05-19
中健智诊(重庆)生物研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, there are very few deep-processed products that can be called giant salamanders.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 proposes a method for extracting collagen peptides for intestinal absorption, comprising the following steps:

[0019] (1) Firstly, the giant salamander skin was cleaned and deodorized by microbial transformation, then soaked in 0.5 mol / L NaOH solution for 35 minutes, and then rinsed with water.

[0020] (2) adding alkaline protease, trypsin, papain and flavor enzyme to the giant salamander skin treated in the step (1) to enzymolyze it for 5 hours at a temperature of 50° C., and then heating it for 10 minutes in a water bath at a temperature of 95° C., Perform enzyme inactivation treatment, then centrifuge and filter at 500r / min for 15min and collect the filter residue. The quality of the alkaline protease is 0.2% of the mass of the giant salamander skin, the quality of the trypsin is 0.3% of the mass of the giant salamander skin, and the quality of the papain is 0.1% of the mass of the giant salamander skin. %, the quality of the flavor enzyme is 0.3% of the...

Embodiment 2

[0023] Example 2 proposes a method for extracting collagen peptides for intestinal absorption, comprising the following steps:

[0024] (1) Firstly, the giant salamander skin was cleaned and deodorized by acid-base salt treatment, then soaked in NaOH solution with a concentration of 2mol / L for 25min, and then rinsed with water.

[0025] (2) adding alkaline protease, trypsin, papain and flavor enzyme to the giant salamander skin treated in the step (1) and enzymatically hydrolyzing it for 4 hours at a temperature of 55° C., and then heating it for 5 minutes in a water bath at a temperature of 100° C., Perform enzyme inactivation treatment, then centrifuge at 3000r / min for 10min and collect the filter residue. The quality of the alkaline protease is 0.3% of the mass of the giant salamander skin, the quality of the trypsin is 0.1% of the mass of the giant salamander skin, and the quality of the papain is 0.2% of the mass of the giant salamander skin. %, the quality of the flavor...

Embodiment 3

[0028] Example 3 proposes a method for extracting collagen peptides for intestinal absorption, comprising the following steps:

[0029] (1) Firstly, the giant salamander skin was cleaned and deodorized by a compound deodorization method, then soaked in NaOH solution with a concentration of 1.25mol / L for 30min, and then rinsed with water.

[0030] (2) Add alkaline protease, trypsin, papain and flavor enzyme to the giant salamander skin treated in the step (1) and enzymolyze it at 53°C for 4.5h, then heat it in a water bath at 98°C for 8min , carry out enzymatic treatment, and then centrifuge at 1500r / min for 13min and collect the filter residue. The quality of the alkaline protease is 0.25% of the mass of the giant salamander skin, the quality of the trypsin is 0.2% of the mass of the giant salamander skin, and the quality of the papain is 0.15% of the mass of the giant salamander skin. %, the quality of the flavor enzyme is 0.25% of the quality of the giant salamander skin. ...

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PUM

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Abstract

The invention relates to a collagen peptide used for intestinal absorption, and an extraction method and application thereof. The method comprises the steps of firstly cleaning andrias davidianus skin, carrying out fishy smell elimination treatment, then soaking the andrias davidianus skin in an NaOH solution, and washing the andrias davidianus skin with water; performing enzymolysis on the treated andrias davidianus skin, performing enzyme deactivation treatment, performing centrifugal filtration, and collecting filter residues; and sequentially carrying out microfiltration, ultrafiltration and nanofiltration on the filter residues, and performing concentrating, freeze-drying and smashing to obtain the collagen peptide used for intestinal absorption. According to the extraction method ofthe collagen peptide used for intestinal absorption, the production cost is reduced, the collagen peptide extracted by adopting the method can repair and regenerate own collagen while supplementing collagen which is lost due to aging problems such as skin collapse, looseness and wrinkles, and the collagen peptide is natural and non-irritating, and has a good free radical scavenging effect, can beused as a daily skin care product for preventing aging, and has a very obvious anti-aging effect.

Description

technical field [0001] The invention relates to a collagen peptide for intestinal absorption, an extraction method and application thereof. Background technique [0002] The giant salamander, commonly known as the giant salamander, belongs to the family Cryptobranchidae of the order Amphibians and is a relatively primitive species among the existing amphibians. It is a living fossil with a long history of 350 million years in my country. It is comparable to giant pandas. It is a traditional medicinal animal in my country. There are also a lot of folk remedies, and the results of modern nutritional composition analysis show that it is rich in nutrition, contains 18 kinds of amino acids, and has a comprehensive composition. With the success of artificial breeding, the importance of giant salamander academic research has been further sublimated, and people's understanding of the edible value and efficacy of giant salamander has continued to deepen. Unfortunately, there are ver...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/34C07K1/14A61K38/39A61K8/65A61P17/18A61Q19/08A23L33/18
CPCC12P21/06C07K14/78A61K8/65A61P17/18A61Q19/08A23L33/18A61K38/00A23V2002/00A23V2200/302A23V2200/318
Inventor 官园园
Owner 中健智诊(重庆)生物研究院
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