Method for synthesizing anthracene fluorescent dye

Abstract

The invention provides a method for synthesizing an anthracene fluorescent dye, which is used for solving the problems of difficult preparation of a key intermediate and low yield when the anthracenefluorescent dye is synthesized. The method has the advantages of easily available raw materials, mild reaction conditions and reduced operation risk. The synthesis method mainly comprises the following six steps: reacting m-halogenated phenol or substituted m-halogenated phenol with formaldehyde in an acidic solution, and purifying to obtain a diphenylmethane derivative, namely a first intermediate product; converting the phenolic hydroxyl group of the first intermediate product into methyl ether or silyl ether to obtain a diphenylmethane derivative with phenolic hydroxyl group protecting groups, namely a second intermediate product; converting the second intermediate product into an organic lithium salt, and reacting the organic lithium salt with a dihalogenated compound to generate an anthracene derivative, namely a third intermediate product; oxidizing the third intermediate product to obtain an anthrone compound, namely a fourth intermediate product; reacting the fourth intermediate product with a Grignard reagent to obtain a fifth intermediate product; and removing the phenolic hydroxyl protecting groups to obtain the fluorescent dye.

Description

technical field [0001] The invention relates to a method for synthesizing anthracene fluorescent dyes in the near-infrared band, and belongs to the field of biochemical synthesis. It is especially suitable for the synthesis of anthracene compounds and heteroatom-containing anthracene compounds. Background technique [0002] With the rapid development of high-throughput sequencing technology in recent years, compared with traditional Sanger sequencing, the biggest advantage is that it can read massive sequence information at the same time. Although the accuracy is not as good as traditional sequencing methods, due to massive data analysis, information beyond the sequence itself can be obtained, such as gene expression, copy number variation, and more accurate readout is an important direction for the development of high-throughput sequencing. [0003] The fluorogenic sequencing method is also widely used in high-throughput sequencing, and fluorogenic sequencing uses terminal...

AI Technical Summary

Problems solved by technology

However, its synthesis yield is relatively low, and the reaction conditions are relatively limited

The present invention adopts a new method for synthesizing anthracene fluorescent dyes to solve the problems of difficult preparation of key intermediates and low yield when synthesizing anthracene fluorescent dyes; at the same time, the raw materials required by the new synthetic route are cheap and easy to obtain, and the reaction conditions are more efficient. Mild, reducing the risk of reaction operation, and less harmful to the environment during the experiment process and experimental treatment

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