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Seed production method of bamboo callus for internal irradiation and chemical mutagenesis fibers

A callus and chemical mutagenesis technology, applied in the field of plant radiation mutagenesis breeding, can solve the problem of not establishing a new set of bamboo germplasm for fiber, immature bamboo genetic transformation system, uncertainty of flowering of bamboo plants, etc. problem, to achieve the effect of fast offspring traits, fast stability and short breeding time

Pending Publication Date: 2020-06-02
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The history and current status of bamboo research in China are relatively ahead of other countries in the world, but due to the complexity of the genetic background of bamboo and the particularity of biology, that is, the characteristics of bamboo plants such as uncertainty in flowering and death after flowering, it is difficult to pass Variety improvement by traditional breeding methods
Existing breeding of fine bamboo varieties for fiber is mainly asexual reproduction from natural mutation selection or introduction and domestication from different places, and the variety type is relatively single
In addition, the genetic transformation system of bamboo is still immature, and it is difficult to genetically improve it through genetic engineering.
So far, no modern biotechnology system has been established for the creation and selection of new bamboo germplasm resources for fiber

Method used

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  • Seed production method of bamboo callus for internal irradiation and chemical mutagenesis fibers
  • Seed production method of bamboo callus for internal irradiation and chemical mutagenesis fibers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A callus with 54% cellulose content and 23% lignin content was selected to induce callus with the branches and shoots of Ci bamboo. Select internal radiation mutagens Na 131 I. Prepare a mutagen solution of 15GBq. The chemical mutagen EI (ethylene imine) was selected and prepared into a 0.6% mutagens solution. A stainless steel continuous syringe was used to inject 20 μL of 15GBq of internal radiation mutagens solution into callus with a size of about 0.05-1.0 cm. The callus injected with internal radiation mutagens was placed in a 0.6% ethyleneimine mutagens solution and shaken for 120 minutes at a shaking speed of 90 rpm. The mutagenized callus was inoculated on the bud induction medium, and buds were grown after 20 days. After the in vitro root induction, complete regenerated plants were obtained after 18 days. The mutant plants were screened by ISSR molecular marker technology, and after 360 days of transplantation, new germplasm for fiber use with excellent proper...

Embodiment 2

[0033] A callus with a cellulose content of 52% and a lignin content of 22% was selected from the callus induced by the branching stem buds of Azalea liangshanensis. Select internal radiation mutagens Na 131 I. Prepare 25GBq mutagen solution. Select the chemical mutagens EI (ethylene imine) and prepare a 1.2% mutagens solution. A stainless steel continuous syringe was used to inject 30 μL of 25GBq internal radiation mutagens solution into callus with a size of about 0.05-1.0 cm. The callus injected with internal radiation mutagens was placed in 1.2% ethyleneimine mutagens solution and shaken for 90 minutes at a shaking speed of 90 rpm. The mutagenized callus was inoculated on the bud induction medium, and buds were grown after 26 days. After in vitro root induction, complete regenerated plants were obtained after 30 days. The mutant plants were screened by ISSR molecular marker technology. After 360 days of transplanting, new germplasm of Liangshan Cizhu for fiber was screene...

Embodiment 3

[0035] Select the callus induced by the branches and shoots of green bamboo fiber with 42% cellulose content and 27% lignin content. Select internal radiation mutagens Na 131 I. Prepare a mutagen solution of 10GBq. Select the chemical mutagens EI (ethylene imine) and prepare a 1.5% mutagens solution. Use a stainless steel continuous syringe to inject 50 μL of 10 GBq of internal radiation mutagens solution into callus with a size of about 0.05 to 1.0 cm. Place the callus filled with internal radiation mutagens in a 1.5% ethyleneimine mutagens solution and shake for 70 minutes at a shaking speed of 90 rpm. The mutagenized callus was inoculated on the bud induction medium, and buds grew after 27 days. After the roots were induced in vitro, complete regenerated plants were obtained after 23 days. The mutant plants were screened by ISSR molecular marker technology. After 360 days of transplantation, new germplasms of green bamboo for fiber were screened.

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Abstract

The invention discloses a seed production method of bamboo callus for internal irradiation and chemical mutagenesis fibers. The speed production method comprises the steps of selecting bamboo branch stem bud induced callus, carrying out internal irradiation by injecting a mutagenic agent, carrying out shock mutagenesis by using a chemical mutagenic agent, performing noculating on a differentiationculture medium for cultivation, and performing culturing by using a rooting culture medium to obtain a complete regeneration plant 15-30 days later, obtaining a mutant plant through screening by using an EST-SSR molecular marker technology, and screening a new bamboo germplasm for the fibers with excellent characters. The seed production method has the characteristics of simple operation, wide breeding materials, stable and fast progeny characters and short breeding time.

Description

Technical field [0001] The invention belongs to the technical field of plant radiation mutation breeding, and specifically relates to a seed production method of bamboo callus for fiber mutation by internal irradiation and chemical mutation. Background technique [0002] The pulp and paper industry is one of the important pillar industries of China and the world economy. With the rapid development of my country's economy, the demand for paper will continue to increase rapidly. Due to the shortage of forest resources in my country and the continuous improvement of laws and policies related to forest protection, as well as the relatively low utilization rate of waste paper recycling in the paper industry chain, the gap in wood resources can only be made up by imports. Import wood and wood pulp without spending a lot of money. However, bamboo, as a plant natural resource widely distributed in my country, has the characteristics of wide distribution, strong adaptability, fast growth,...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/06
CPCA01H1/06A01H4/008
Inventor 胡尚连罗学刚曹颖黄艳王博雅
Owner SOUTHWEAT UNIV OF SCI & TECH
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