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Type I collagen with antigenicity removed, its preparation method and application, and coconut sugar and its preparation method

A collagen and antigenic technology, applied in the field of coconut candy and its preparation, can solve the problems such as no reports on the de-antigenicity treatment of products, and achieve the effects of eliminating enzyme treatment steps, removing antigenicity and saving costs.

Active Publication Date: 2020-11-24
JIANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on collagen health care products is mostly concentrated on the product preparation method, but the research on the deantigenization treatment of the product has not been reported

Method used

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  • Type I collagen with antigenicity removed, its preparation method and application, and coconut sugar and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0035] According to the present invention, the collagen I may be various commercially available type I collagens, or self-prepared type I collagens. According to a preferred embodiment of the present invention, the type I collagen is type I collagen from freshwater fish.

[0036] According to a preferred embodiment of the present invention, the preparation method of the type I collagen from freshwater fish comprises:

[0037](i) Alkali immersion, organic solvent immersion and acid immersion are performed on the freshwater fish skin sequentially, and the material after acid immersion is subjected to solid-liquid separation to obtain a liquid phase;

[0038] (ii) salting out the liquid phase obtained in step (i), and subjecting the obtained salted out product to solid-liquid separation to obtain a solid phase;

[0039] (iii) dissolving the solid phase in acid, and then sequentially dialyzing the acid solution and water to obtain the type I collagen from freshwater fish.

[004...

preparation example

[0082] This preparation example is used to illustrate the preparation method of type I collagen

[0083] (1) Clean and remove impurities from freshwater fish skin, cut it into 0.5cm*0.5cm minced fish skin, add 20 times the volume of 0.1mol / L NaOH solution, stir, extract for 36 hours, change the solution every 6 hours, Remove impurities and pigments;

[0084] (2) Wash the fish skin after alkali dipping repeatedly with distilled water until the eluate is neutral;

[0085] (3) After draining, add 20 times the volume of 10% n-butanol by volume, stir, extract for 24 hours, change the liquid once every 6 hours, and remove the fat in the fish skin;

[0086] (4) Wash the fish skin repeatedly with distilled water, add 30 times the volume of 0.5mol / L acetic acid solution after draining, stir, after leaching for 72 hours, centrifuge at 8000rmp for 15min, and take the supernatant;

[0087](5) Add pre-ground NaCl powder for salting out, stir until the final salt concentration is 0.8mol / L...

Embodiment 1-1

[0090] This example is used to illustrate the method for removing the antigenicity of type I collagen

[0091] (1) Mix 100 parts by weight of type I collagen prepared in the preparation example with 100 parts by weight of deionized water, and magnetically stir until fully dissolved;

[0092] (2) Process the solution of step (1) with a dynamic high-pressure micro-jet, and circulate 3 times under a pressure of 120MPa;

[0093] (3) Add 100 parts by weight of glucose to the solution in step (2), and magnetically stir until fully dissolved;

[0094] (4) After freeze-drying the solution in step (3), react at 70° C. and 75% relative humidity for 6 hours to obtain antigen-depleted type I collagen.

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Abstract

The invention belongs to the field of functional leisure food, and in particular relates to type I collagen with antigenicity removed, its preparation method and application, coconut sugar and its preparation method. The method for removing the antigenicity of type I collagen comprises: subjecting the type I collagen solution to dynamic high-pressure micro-jet treatment to obtain the treated type I collagen solution; uniformly mixing the treated type I collagen solution with glucose to obtain The mixed solution; the mixed solution is freeze-dried and reacted at 50-100° C. and 60-85% relative humidity to remove type I collagen antigenicity. Adopting the method of the invention can better remove the antigenicity of type I collagen, and the collagen coconut sugar prepared thereby can have wider consumers.

Description

technical field [0001] The invention belongs to the field of functional leisure food, and specifically relates to a method for removing the antigenicity of type I collagen, and the type I collagen for removing antigenicity prepared by the method, and the type I collagen for removing antigenicity is used in the preparation of food , applications in cosmetics or biomedical materials, and a coconut sugar and a preparation method thereof. Background technique [0002] Fish collagen is a source of high-quality collagen, but immunoglobulin E in fish collagen can cause allergic phenomena such as vomiting and diarrhea; type I collagen has been proven to have strong allergenicity, with 50 About % of patients allergic to fish are allergic to type I collagen. Type I collagen is composed of two α1 subunits and one α2 subunit in a triple helical structure with a molecular mass of about 300kDa; type I collagen has good biocompatibility, easy biodegradation, non-toxicity and stable struct...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78C07K1/107A23G3/48A23G3/42A23G3/44
CPCA23G3/42A23G3/44A23G3/48C07K14/78
Inventor 涂宗财李鑫刘俊胡月明王辉沙小梅
Owner JIANGXI NORMAL UNIV
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