Check patentability & draft patents in minutes with Patsnap Eureka AI!

Polypeptide composition for regulating cell membrane receptor as well as preparation method and application thereof

A polypeptide composition and cell membrane technology, applied in the field of medicine, can solve the problems of physical characteristics, immunogenicity, biocompatibility, application limitations, etc., and achieve the effects of shortening molecular size, improving stability and permeability, and efficient interaction

Active Publication Date: 2020-06-05
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
View PDF8 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the physical characteristics of the stimulus, the immunogenicity and biocompatibility of the carrier, its application in vivo is limited.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polypeptide composition for regulating cell membrane receptor as well as preparation method and application thereof
  • Polypeptide composition for regulating cell membrane receptor as well as preparation method and application thereof
  • Polypeptide composition for regulating cell membrane receptor as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] This embodiment provides a polypeptide composition for regulating cell membrane receptors and a preparation method thereof; the polypeptide composition includes a targeting fragment (TA) and an assembly driving fragment (SA).

[0064] 1. Design of targeting fragment (TA) and assembly driving fragment (SA)

[0065] Design polypeptide molecules with target recognition and assembly behaviors at both ends, respectively label the click reaction group DBCO and azide, and realize the coupling of two polypeptide chains through bioorthogonal reactions in vivo to construct target polypeptide molecules.

[0066] Wherein, the targeting fragment includes a polypeptide molecule with a targeting recognition function, its sequence is ATYSEFPGNLKPC, and the click (click) reaction group DBCO; the structure of the targeting fragment can be expressed as ATYSEFPGNLKPC-DBCO;

[0067] The design of the assembly unit adopts a modular design, including: 1) an amyloid assembly unit with self-ass...

Embodiment 2

[0083] In this example, the molecular assembly behavior of the polypeptide compositions TA and SA prepared in Example 1 mediated by the click reaction was studied in an aqueous solution.

[0084] Based on the theory of light scattering, the turbidity changes of TA and SA in phosphate buffer solution were tracked in real time by ultraviolet spectrophotometer. The specific steps are:

[0085] First detect the 50×10 6 The targeting fragment TA of M and 50×10 6 The assembly of M drives the absorbance of the fragment SA (detection wavelength is 500nm) over time, and draws the time-absorbance curve of TA and SA in the buffer to determine the monodispersity of the two polypeptide fragments within a certain time range State stability.

[0086] Secondly, after co-incubating the equimolar ratio of TA and SA, the change of its absorbance was observed, and the time-absorbance change curve was drawn to evaluate the aggregation of the two molecules after the reaction. At the same time, t...

Embodiment 3

[0091] This example studies the regulation of receptors by polypeptide compositions at the cellular level.

[0092] The synthesis method of the polypeptide composition is the same as that in Example 1, except that the signal molecule is replaced by the fluorescent molecule FITC to obtain the polypeptide compositions TA and SA.

[0093] Dendritic cells, as important antigen-presenting cells, play a key role in the activation of T cells. CD40 is a membrane receptor expressed on the surface of dendritic cells and plays an important role in the maturation of DCs. Therefore, the cells selected in this embodiment are DC2.4 cells (dendritic cells derived from mouse bone marrow), and FITC-labeled TA is 50×10 -6 The concentration of M was incubated with DC2.4 cells for 30 min, washed 3 times with PBS, and the prepared samples were observed with a laser confocal microscope (multi-beam laser confocal imaging system, UltraVIEW VoX (U-Vox), Perkin El Mo Instrument (Shanghai) Co., Ltd.) m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a polypeptide composition for regulating a cell membrane receptor as well as a preparation method and application of the polypeptide composition. The polypeptide composition comprises a targeting fragment and an assembly driving fragment, wherein the targeting fragment comprises a targeting unit and a reaction group I which are connected through a chemical bond, and a receptor of the targeting unit is a cell membrane receptor; and the assembly driving fragment comprises a reaction group II, an assembly unit and a signal molecule which are sequentially connected through chemical bonds. Polypeptide with good biocompatibility is used as a medium for regulating and controlling a receptor, the polypeptide composition is designed in two sections and is respectively designed into a targeting fragment modified with a click reaction group and an assembly driving fragment, the targeting fragment can be specifically combined to a target receptor, and coupling with the assembly driving fragment is realized through a click reaction. Meanwhile, fibers are assembled on a cell membrane, so that oligomerization of the cell membrane receptor is realized, and accurate regulation and control of the receptor are completed.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a polypeptide composition for regulating cell membrane receptors, a preparation method and application thereof. Background technique [0002] From DNA transcription to protein translation, from cellular signal transduction to the construction of subcellular organelles, the assembly of biomolecules plays a key role in many biological processes in cells. Peptides, as an important biomolecule, also have a certain ability to assemble. Compared with other biomolecules, polypeptides are able to interact in hydrogen bonds, hydrophilic-hydrophobic interactions due to their smaller molecular size, residue polarity, and hydrophobicity. The assembly occurs driven by various non-covalent interactions such as interaction, and thus becomes the unit of choice for constructing supramolecular structures. [0003] Organisms regulate their functions through signal transduction between cells. Acc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K19/00C07K1/16C07K1/06C07K1/04
CPCC07K7/08C07K2319/33
Inventor 王浩穆合塔尔江·马木提王羿安红维王杰
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com