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Preparation method and application of stem cell composite exosome

A technology for exosomes and stem cells, which is applied in the field of preparation of stem cell composite exosomes, and achieves the effects of convenient use, long storage time and convenient material extraction.

Inactive Publication Date: 2020-06-12
陕西中延再生医学研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in the existing related reports, exosomes derived from umbilical cord mesenchymal stem cells combined with endometrial stem cell exosomes have not been able to be applied to the treatment of gynecological diseases and the repair of tissue lesions.

Method used

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  • Preparation method and application of stem cell composite exosome
  • Preparation method and application of stem cell composite exosome
  • Preparation method and application of stem cell composite exosome

Examples

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preparation example Construction

[0039] see Figure 1-3 , the present invention provides a technical solution: a method for preparing exosomes composed of stem cells, specifically a method for preparing exosomes composed of umbilical cord mesenchymal stem cells and endometrial stem cells, comprising the following steps:

[0040] S1. Preparation of exosomes from umbilical cord mesenchymal stem cells:

[0041] 1) Select P3-P6 umbilical cord mesenchymal stem cells from the cell bank for recovery and perform starvation culture;

[0042] 2) collecting the cell culture supernatant, centrifuging, filtering, and adding polyethylene glycol for incubation;

[0043] 3) After incubation, centrifuge, discard the supernatant, resuspend the pellet with PBS buffer, add polyethylene glycol, and incubate again. After centrifugation, resuspend the pellet with PBS buffer, and determine the protein content and quality safety Evaluation, that is, exosomes from umbilical cord mesenchymal stem cells;

[0044]S2. Preparation of en...

Embodiment example 1

[0063] Patient Li, 36 years old, had an endometrium thickness of 7mm after induced abortion and curettage, which was clinically diagnosed as thin endometrium.

[0064] The patient was treated with umbilical cord mesenchymal stem cells and endometrial stem cells compound exosomes eight times through a disposable medical applicator, and 5ml of the product was injected into the uterus each time, and the treatment was once a week. Two months later (4 days after menstruation), the thickness of the endometrium reached 8.8mm, the endometrial damage was effectively treated, and the thin endometrium was gradually recovering.

Embodiment example 2

[0066] Patient Zhang, 42 years old, had damaged skin barrier function, dry skin, obvious wrinkles, obvious pigmentation, and prone to chapped folliculitis and other symptoms. The clinical diagnosis is impaired skin barrier function and sensitive skin.

[0067] The patient was treated with umbilical cord mesenchymal stem cells and endometrial stem cells compound exosomes for six times, each time injecting 3ml of the product with a water light needle, and treated once a week. After one and a half months, the client's skin was moisturized and elastic, inflammation was significantly improved, and wrinkles Significantly reduced, dark spots are also lightened, the customer is very satisfied, and the treatment effect is ideal.

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Abstract

The invention discloses a preparation method of stem cell composite exosome, and particularly relates to a preparation method of umbilical cord mesenchymal stem cell and endometrial stem cell composite exosome. The method comprises the following steps: S1, preparation of umbilical cord mesenchymal stem cell exosome; s2, preparation of endometrial stem cell exosome; and S3, preparation of the umbilical cord mesenchymal stem cell and endometrial stem cell composite exosome by mixing the umbilical cord mesenchymal stem cell exosome with the endometrial stem cell exosome, adding a protective agent, and performing low-temperature freeze drying treatment to obtain stem cell exosome freeze-dried powder. The umbilical cord mesenchymal stem cell and endometrial stem cell exosome composite growth factor prepared by the method disclosed by the invention has the advantages of no pain, no surgery, no operation and no side effect for solving the problem of female reproductive health, can be used athome in combination with hospital detection, and is safe and efficient.

Description

technical field [0001] The invention relates to the field, specifically a method for preparing stem cell compound exosomes and its application. Background technique [0002] Gynecological operations such as abortion and curettage have brought great harm to women's endometrium damage, resulting in more and more infertility and frequent gynecological diseases. Stem cells play an important role in tissue repair and regenerative medicine, and have been applied in the treatment of gynecological diseases. Studies have shown that the tissue repair and regeneration functions of stem cells are mainly realized by their paracrine effects, and exosomes are one of the important paracrine factors. How to quickly prepare highly active exosomes and apply them to gynecological diseases There are few reports on the treatment. [0003] Umbilical cord mesenchymal stem cells release a large number of exosomes to build a homeostatic microenvironment for tissue repair, thereby eliminating inflam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C12N5/074A01N1/02A61K35/28A61P15/00A61P15/08A61P19/02A61P17/00
CPCA01N1/0221A01N1/0284A61K35/28A61P15/00A61P15/08A61P17/00A61P19/02C12N5/0668C12N5/0682C12N2509/00
Inventor 杨永利
Owner 陕西中延再生医学研究院有限公司
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