Preparation method and application of functionalized graphene targeted sterilization material with synergistic effect of photo-thermal treatment and chemotherapy

A synergistic, photothermal technology, applied in the fields of wave energy or particle radiation treatment materials, medical preparations without active ingredients, and medical preparations containing active ingredients, etc. problems, to achieve the effect of promoting wound healing, improving antibacterial efficiency, and simple operation

Active Publication Date: 2020-06-16
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] CN109876141A discloses a preparation method and application of a boric acid functionalized graphene material with targeted photothermal sterilization properties. By selecting carboxylated graphene, 3-aminopyridine is introduced through covalent crosslinking based on the condensation reaction of amino and carboxyl groups. Then, based on the quaternization reaction, 3-bromomethylphenylboronic acid was introduced, and boric acid groups and quaternary ammonium salt groups were grafted to obtain a boric acid-functionalized graphene material with targeted photothermal sterilization properties, which can only achieve a single light Heat therapy, limited range of application

Method used

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  • Preparation method and application of functionalized graphene targeted sterilization material with synergistic effect of photo-thermal treatment and chemotherapy
  • Preparation method and application of functionalized graphene targeted sterilization material with synergistic effect of photo-thermal treatment and chemotherapy
  • Preparation method and application of functionalized graphene targeted sterilization material with synergistic effect of photo-thermal treatment and chemotherapy

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Embodiment 1

[0037] Embodiment 1, a method for preparing a functionalized graphene-targeted bactericidal material with synergistic effects of photothermal and chemotherapy, which includes the following steps.

[0038] In step 1, 2 mg of mercaptographene, 0.5 mg of nitroso-tert-butyl ester and 5 ml of dimethyl sulfoxide were evenly mixed, and allowed to stand at room temperature in the dark for 12 hours to obtain a mixed solution.

[0039] Step 2: Centrifuge the mixed solution at a rotational speed of 11000 r / min for 90 minutes, take the sediment at the bottom and remove the supernatant to obtain the product.

[0040] In step 3, the product was repeatedly washed three times with deionized water, the supernatant was removed, and then centrifuged at a rotational speed of 11000 r / min for 90 min.

[0041] Step 4, resuspend the product obtained in Step 3 with 15 ml of HEPES to obtain suspension A, add 1 mg of 4-mercaptophenylboronic acid to suspension A, and stir at room temperature for 12 hours t...

Embodiment 2

[0043] Embodiment 2, a method for preparing a functionalized graphene-targeted bactericidal material with synergistic effects of photothermal and chemotherapy, which includes the following steps.

[0044] In step 1, 4 mg of mercaptographene, 0.5 mg of nitroso tert-butyl ester and 5 ml of dimethyl sulfoxide were evenly mixed, and allowed to stand at room temperature in the dark for 12 hours to obtain a mixed solution.

[0045] Step 2: Centrifuge the mixed solution at a rotational speed of 13000 r / min for 80 minutes, take the sediment at the bottom and remove the supernatant to obtain the product.

[0046] In step 3, the product was repeatedly washed three times with deionized water, the supernatant was removed, and then centrifuged at a rotational speed of 13000 r / min for 60 min.

[0047] Step 4, resuspend the product obtained in Step 3 with 15 ml of HEPES to obtain suspension A, add 1 mg of 4-mercaptophenylboronic acid to suspension A, and stir at room temperature for 12 hours...

Embodiment 3

[0049] Embodiment 3, a method for preparing a functionalized graphene-targeted bactericidal material with synergistic effects of photothermal and chemotherapy, which includes the following steps.

[0050] In step 1, 4 mg of mercaptographene, 1 mg of nitroso tert-butyl ester and 10 ml of dimethyl sulfoxide were evenly mixed, and allowed to stand at room temperature in the dark for 14 hours to obtain a mixed solution.

[0051] Step 2: Centrifuge the mixed solution at a rotational speed of 11000 r / min for 60 minutes, take the sediment at the bottom and remove the supernatant to obtain the product.

[0052] In step 3, the product was repeatedly washed three times with deionized water, the supernatant was removed, and then centrifuged at a rotational speed of 11000 r / min for 60 min.

[0053] Step 4: resuspend the product obtained in Step 3 with 15 ml of HEPES to obtain suspension A, add 4 mg of 4-mercaptophenylboronic acid to suspension A, and stir at room temperature for 12 hours ...

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Abstract

The invention discloses a preparation method and application of a functionalized graphene targeted sterilization material with a synergistic effect of photo-thermal treatment and chemotherapy, and thepreparation method comprises the following steps: step 1, uniformly mixing sulfhydrylated graphene, nitroso-tert-butyl ester and dimethyl sulfoxide, and standing in a dark place at room temperature to obtain a mixed solution; step 2, carrying out centrifugal treatment, and removing the supernatant to obtain a product; 3, washing the product with deionized water, and then carrying out centrifugaltreatment; 4, resuspending the product obtained in the step 3 with HEPES to obtain a suspension A, adding 4-mercaptophenylboronic acid, and stirring at room temperature to obtain a suspension B; and 5, carrying out centrifugal treatment on the suspension B, and washing with deionized water to obtain the functionalized graphene targeted sterilization material with the synergistic effect of photothermal treatment and chemotherapy. The invention has efficient targeted photothermal and chemotherapy synergistic bacterium killing capacity, is good in biocompatibility and simple in preparation method, and can effectively treat multi-drug-resistant gram-negative bacillus skin wound infection and promote healing of skin infection wounds.

Description

technical field [0001] The invention relates to a functionalized graphene material, in particular to a preparation method and application of a functionalized graphene targeted bactericidal material with synergistic effects of photothermal and chemotherapy. Background technique [0002] Bacterial infection of skin wounds has always been a major challenge in the treatment of burns and wounds. At present, bacterial infections still mainly rely on antibiotic treatment, but clinical antibiotic treatment is often accompanied by the emergence and prevalence of multi-drug resistant strains. Due to the lack of new antibiotics, some Pan-drug-resistant strains, especially multidrug-resistant Gram-negative bacilli, even appear to be "drug-resistant". Multidrug-resistant Gram-negative bacilli infection in skin wounds has become a thorny problem in clinical anti-infection treatment. Because traditional antibiotic treatment is difficult to work, we urgently need to find some new treatment ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K31/21A61K47/52A61P17/00A61P31/04
CPCA61K41/0052A61K31/21A61K47/52A61P17/00A61P31/04A61K2300/00
Inventor 钱卫赵保华董文静王鹤李海胜王颖罗高兴贺伟峰
Owner THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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