Biocompatible and degradable electrospinning asiaticoside, its preparation method and application

A technology of asiaticoside and electrospinning technology, which is applied in the field of biomaterials containing asiaticoside, can solve the problems of accelerated degradation of PLGA, influence of PLGA scaffold-mediated regeneration ability, excessive proliferation of pathological cells, etc., and achieve hydrophilicity Improved effect

Active Publication Date: 2022-04-15
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Application Information

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Problems solved by technology

However, PLGA itself and its degradation reactions and degradation products after implantation in the body can lead to severe inflammatory reactions in the body, leading to high infiltration of inflammatory cells such as dendritic cells, mast cells, granulocytes and macrophages, etc., resulting in PLGA Accelerated degradation and excessive proliferation of pathological cells, thereby affecting the regenerative capacity mediated by PLGA scaffolds

Method used

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  • Biocompatible and degradable electrospinning asiaticoside, its preparation method and application
  • Biocompatible and degradable electrospinning asiaticoside, its preparation method and application
  • Biocompatible and degradable electrospinning asiaticoside, its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 spinning characteristic investigation

[0030] After spinning and gold-spraying, it was observed with a scanning electron microscope. Such as figure 1 Shown, the morphology of non-drug-loaded PLGA electrospinning (PLGA) and drug-loaded PLGA electrospinning (AS-PLGA). Such as figure 2 As shown, the electrospun diameter of unloaded PLGA was found to be 1265 ± 170.5 nm, and the average diameter of drug-loaded PLGA was 1269 ± 188.2 nm, which were similar. This diameter can significantly promote the proliferation and spreading of fibroblasts and the expression of collagen, which is more conducive to the repair of wounds.

[0031] Mechanical analysis of the maximum load, Young's modulus, mechanical tension and stress-strain curve of the two showed that the mechanical strength and performance of PLGA after drug loading were slightly weakened.

[0032] Fourier transform infrared spectroscopy (FTIR) was applied to characterize the properties of unloaded and dru...

Embodiment 2

[0034] Embodiment 2 spinning function verification

[0035] SD rats were randomly divided into two groups: non-drug-loaded PLGA electrospins and drug-loaded PLGA electrospins were implanted, respectively. SD rats were anesthetized with 10% chloral hydrate (4 ml / kg). Two PLGA electrospins were compressed into a cylindrical shape (diameter 9 mm, thickness 1 mm). Two incisions with a length of 1 cm were made on both sides of the rat spine. After the subcutaneous tissue was separated, PLGA and drug-loaded PLGA were respectively embedded, and then the wound was closed with sutures. Materials collected at 2 and 4 weeks after surgery were observed by HE staining and immunohistochemical staining. It was found that the inflammatory response produced by the host in the drug-loaded PLGA group was significantly weakened, and the material degradation was also slightly reduced. Discovered by staining for macrophage phenotypic proteins, such as Figure 5A with Figure 5B As shown, the i...

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Abstract

A kind of spinning, which is made by blending PLGA, PEO and asiaticoside through stable jet electrospinning technology, and has the same orientation. Wherein, the weight ratio of PLGA to PEO is 4:1, and the dosage of asiaticoside is 10wt% of PLGA. The spinning of the present invention has significantly improved hydrophilicity, has the effect of regulating macrophage phenotype transformation and anti-inflammation in vivo, and can be used to prepare medicines or medical devices for regulating macrophage phenotype transformation and anti-inflammation in vivo (such as: stand).

Description

technical field [0001] The invention relates to a biological material, in particular to a biological material containing asiaticoside, which is biodegradable and biocompatible, as well as its preparation method and application. Background technique [0002] Madecassoside, also known as asiaticoside, is light yellow to light brown powder; odorless, bitter, slightly hygroscopic, and has the effect of promoting wound healing. It is mainly used to treat trauma, surgical trauma, burns, keloids and scleroderma. [0003] PLGA (polylactic acid glycolate) has excellent biocompatibility, good mechanical properties and adjustable biodegradation time, and is widely used as a synthetic material for tissue engineering. PLGA electrospinning and scaffolds have been widely used in tissue engineering for tissue construction, such as bone, cartilage, skin, and nerve regeneration, and for drug delivery systems. However, PLGA itself and its degradation reactions and degradation products after ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D01F8/14D01F8/16D01F1/10A61L27/18A61L27/54A61L27/58
CPCD01F8/14D01F8/16D01F1/10A61L27/18A61L27/54A61L27/58A61L2300/23A61L2300/41
Inventor 黄佳沈炎冰张彦中刘伟
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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