Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for detecting acrylamide in grain product

A detection method and technology of acrylamide, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of small molecular weight, limitation, and large interference of acrylamide

Pending Publication Date: 2020-06-16
ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the LC-MS / MS method is limited by expensive instruments, it is difficult to popularize, and the molecular weight of acrylamide is small, and the interference is large
The two standard methods of GC-MS adopt derivatization after purification. Acrylamide is very soluble in water and difficult to concentrate and purify, resulting in long detection time and high detection limit.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting acrylamide in grain product
  • Method for detecting acrylamide in grain product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] (1) Reagents and materials

[0081] Note: Unless otherwise specified, the reagents used in the present invention are all produced by Shanghai Sinopharm, all of which are of analytical grade, and the water is ultrapure water.

[0082] Sodium thiosulfate, potassium bromate, potassium bromide, concentrated sulfuric acid, absolute ethanol, petroleum ether: boiling point 30-60°C;

[0083] n-Hexane, ethyl acetate: chromatographically pure, methanol: chromatographically pure, acrylamide standard: purity greater than 99%, 13 C 3 -Acrylamide internal standard solution: 100μg / mL, SCP solid phase extraction column: specification 200mg / 6mL.

[0084] (2) Standard solution and reagent preparation

[0085] Acrylamide standard stock solution (1.0mg / mL): Weigh 10mg of acrylamide standard into a 10mL volumetric flask, dissolve in methanol and dilute to the mark, and store in a 4°C refrigerator.

[0086] Acrylamide standard solution (10.0μg / mL): Take 0.25mL of 1.0mg / mL acrylamide stan...

Embodiment 2

[0127] Add 0.01mg / kg acrylamide to infant supplementary food rice flour (rice flour contains acrylamide at a lower concentration) and detect according to the method of Example 1, the recovery rate of acrylamide is 74.5%, and the signal-to-noise ratio (S / N) It is 22.7, shows that the detection method of the present invention has high sensitivity, can accurately measure the content of acrylamide in the grain sample that acrylamide content is 0.01mg / kg, so it is determined that the quantitative limit of the detection method of the present invention is 0.01mg / kg, according to 1 / 3 Quantitative limit calculation The detection limit of acrylamide in grain products is 0.003mg / kg.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Granularityaaaaaaaaaa
Ionization energyaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for detecting acrylamide in a grain product. The method comprises the steps of: mixing the grain product, an acrylamide internal standard solution and an extracting agent to obtain an extraction solution; carrying out bromination derivation on the obtained extraction solution to obtain a supernatant; purifying the obtained supernatant to obtain a test solution; andcarrying out gas chromatography-mass spectrometry analysis on the obtained test solution to obtain the content of acrylamide. According to the method, a method of first derivation and then purification is adopted, and bromination derivation is firstly carried out on the extraction solution, so that bromine reacts with acrylamide in the extraction solution to generate 2, 3-dibromopropionamide, thusmaking the acrylamide derivative easily adsorbed by a solid-phase extraction column, and shortening the detection time; and then performing purification treatment, thus improving the concentration and purification effect, and lowering the acrylamide detection limit. The result of the embodiment shows that the detection time of the detection method provided by the invention is about 90min, the quantification limit is 0.01mg / kg, and the detection limit is 0.003mg / kg.

Description

technical field [0001] The invention relates to the technical field of food safety, in particular to a method for detecting acrylamide in food products. Background technique [0002] Acrylamide was assessed as a carcinogenic compound by the International Agency for Research on Cancer (IARC) in 1994. In April 2002, the Swedish National Food Administration announced for the first time that scientists from Stockholm University in Sweden detected the possibility of carcinogenicity in starchy foods such as French fries, potatoes, and bread that have been fried at high temperatures for a long time. Acrylamide; in May, the British Food Standards Agency (BFSA) published similar results. Subsequently, government agencies responsible for food safety in Norway, the United States, Australia, New Zealand, Canada and other countries also measured the content of acrylamide in food and published the results, and the findings of Swedish scientists were confirmed. At present, there are no r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/88G01N30/06G01N30/08
CPCG01N30/88G01N30/06G01N30/08G01N2030/085G01N2030/065
Inventor 吴平谷
Owner ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION