Rapid detection kit and method for inactivating novel coronavirus

A detection kit and fast technology, applied in the biological field, can solve the problems of infection, undetectability, and easy destruction of medical staff and testing personnel, and achieve the effect of reducing turnaround time, reducing pressure, and accurate detection results

Active Publication Date: 2020-06-23
浙江大学医学院附属第四医院
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

They all need to collect fresh samples (pharyngeal swabs, sputum, lung perfusion fluid or blood) from patients for testing. Since the new coronavirus is very contagious, this will cause great harm to the medical staff and testing personnel who collect the samples. risk of infection
Immediately inactivating the collected samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rapid detection kit and method for inactivating novel coronavirus
  • Rapid detection kit and method for inactivating novel coronavirus
  • Rapid detection kit and method for inactivating novel coronavirus

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0042] Example 1:

[0043] A rapid detection kit for inactivating the new coronavirus, including the following components:

[0044] (1) Inactivation reaction test tube (2mL test tube), containing 100μg alkaline trypsin lyophilized powder,

[0045] (2) Enzymatic hydrolysis reaction solution: ammonium bicarbonate with a concentration of 500mmol / L, mercaptoethanol with a concentration of 50mmol / L, and isotope-labeled long polypeptide with a concentration of 100ng / mL; the amino acid sequence of the isotope-labeled long polypeptide is: PQSNQRSAPRI*TFGGPTDSTDNNQNGGRNGARPKQRRP( SEQ ID No. 10), where I is [ 13 C 6 , 15 N]-Isoleucine.

[0046] (3) Rapid detection standard polypeptide; the amino acid sequence of the rapid detection standard polypeptide is: ITFGGPTDSTDNNQNGGR (SEQ ID No. 1). The concentration of the rapid detection standard peptide is 100ng / ml (aqueous solution).

[0047] The present invention uses a new type of coronavirus (SARS-CoV-2, positive samples are collected by our hosp...

Example Embodiment

[0048] Example 2:

[0049] A non-medical use method for rapid detection of new coronavirus that reduces the risk of infection among medical staff, including the following steps:

[0050] (1) Open the kit, add 1 mL of the enzymatic hydrolysis reaction solution to a 2 mL inactivation reaction tube containing 100 μg of alkaline trypsin lyophilized powder, shake it well, and the medical staff will collect the patient’s throat swab and quickly put it into inactivation. In the reaction test tube; at the same time, the rapid detection standard peptide is used as the standard substance in the laboratory, and liquid chromatography tandem mass spectrometry is used as the detection instrument, and the sample is injected and analyzed to establish a standard map;

[0051] (2) On the way back to the laboratory, put the inactivation reaction tube into a portable incubator (commercially available) and incubate at 37°C for 30-60 minutes (depending on the distance from the laboratory, the longer the i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a rapid detection kit for inactivating novel coronavirus. The kit comprises the following components (1) an inactivation reaction test tube containing a trypsin freeze-dried powder, (2) an enzymolysis reaction solution, and (3) a rapid detection standard polypeptide. An amino acid sequence of the rapid detection standard polypeptide is ITFGGPTDSTDNNQNGGR. Under the condition of ensuring detection sensitivity and specificity, inactivated viruses can be quickly detected, an accurate detection result is obtained, and an infection risk of medical personnel and detection personnel is reduced to the maximum extent.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to a rapid detection kit and method for inactivating the new coronavirus. Background technique [0002] Viruses are a class of non-cellular microorganisms. The mature and complete virus particle becomes the virion, which is composed of nucleic acid and protein. Viruses are characterized by their small size, which can pass through a sterile filter; simple structure, containing only one type of nucleic acid (DNA or RNA); lack of genes encoding mitochondria and ribosomes, and must be parasitic in living cells and replicated Breed offspring. [0003] Coronaviruses belong to the genus Coronavirus of the order Nidovirales, the family Coronaviridae, and the genus Coronavirus in systematic classification. Viruses of the genus Coronavirus are RNA viruses with an envelope and a linear single-stranded positive strand genome. They are a large class of viruses that exist widely in nature....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02G01N30/36G01N30/86G01N33/68
CPCG01N30/02G01N30/36G01N30/8679G01N33/6848
Inventor 吴建国朱维罡吴伟根吴英萍李游江
Owner 浙江大学医学院附属第四医院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap