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Rapid detection kit and method for inactivating novel coronavirus

A detection kit and fast technology, applied in the biological field, can solve the problems of infection, undetectability, and easy destruction of medical staff and testing personnel, and achieve the effect of reducing turnaround time, reducing pressure, and accurate detection results

Active Publication Date: 2020-06-23
浙江大学医学院附属第四医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

They all need to collect fresh samples (pharyngeal swabs, sputum, lung perfusion fluid or blood) from patients for testing. Since the new coronavirus is very contagious, this will cause great harm to the medical staff and testing personnel who collect the samples. risk of infection
Immediately inactivating the collected samples can effectively reduce the risk of infection for medical staff and testing personnel. However, after the samples are inactivated (usually using an autoclave), although the virus loses its original pathogenicity, its Detection markers (DNA, RNA, and proteins with spatial structure) are also easily damaged and cannot be detected

Method used

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  • Rapid detection kit and method for inactivating novel coronavirus
  • Rapid detection kit and method for inactivating novel coronavirus
  • Rapid detection kit and method for inactivating novel coronavirus

Examples

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Embodiment 1

[0043] A rapid detection kit for inactivating the new coronavirus, comprising the following components:

[0044] (1) Inactivation reaction test tube (2mL test tube), containing 100 μg alkaline trypsin freeze-dried powder,

[0045] (2) Enzymolysis reaction solution: ammonium bicarbonate with a concentration of 500mmol / L, mercaptoethanol with a concentration of 50mmol / L, and isotope-labeled long polypeptide with a concentration of 100ng / mL; the amino acid sequence of the isotope-labeled long polypeptide is: PQSNQRSAPRI*TFGGPTDSTDNNQNGGRNGARPKQRRP( SEQ ID No.10), wherein I is [ 13 C 6 , 15 N]-isoleucine.

[0046] (3) Rapid detection standard polypeptide; the amino acid sequence of the rapid detection standard polypeptide is: ITFGGPTDSTDNNQNGGR (SEQ ID No.1). The concentration of the rapid detection standard polypeptide is 100ng / ml (aqueous solution).

[0047] The present invention uses a new type of coronavirus (SARS-CoV-2, positive samples collected by the hospital itself) ...

Embodiment 2

[0049] A method for rapid detection of new coronavirus for non-medical use to reduce the risk of infection of medical staff, comprising the following steps:

[0050] (1) Open the kit, add 1mL of the enzymatic hydrolysis reaction solution into a 2mL inactivation reaction test tube containing 100μg of alkaline trypsin freeze-dried powder, shake well, and the medical staff collects the patient's throat swab on the spot and quickly puts it into the inactivation reaction tube. In the reaction test tube; at the same time, in the laboratory, the rapid detection standard polypeptide is used as the standard substance, and the liquid chromatography tandem mass spectrometer is used as the detection instrument to inject samples for analysis and establish a standard map;

[0051] (2) On the way back to the laboratory, put the inactivation reaction test tube into a portable incubator (commercially available), and incubate at 37°C for 30-60 minutes (depending on the distance from the laborato...

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Abstract

The invention discloses a rapid detection kit for inactivating novel coronavirus. The kit comprises the following components (1) an inactivation reaction test tube containing a trypsin freeze-dried powder, (2) an enzymolysis reaction solution, and (3) a rapid detection standard polypeptide. An amino acid sequence of the rapid detection standard polypeptide is ITFGGPTDSTDNNQNGGR. Under the condition of ensuring detection sensitivity and specificity, inactivated viruses can be quickly detected, an accurate detection result is obtained, and an infection risk of medical personnel and detection personnel is reduced to the maximum extent.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to a rapid detection kit and method for inactivating the new coronavirus. Background technique [0002] Viruses are a class of non-cellular microorganisms. The mature and complete virus particle becomes the virion, which is composed of nucleic acid and protein. Viruses are characterized by their small size, which can pass through a sterile filter; simple structure, containing only one type of nucleic acid (DNA or RNA); lack of genes encoding mitochondria and ribosomes, and must be parasitic in living cells and replicated Breed offspring. [0003] Coronaviruses belong to the genus Coronavirus of the order Nidovirales, the family Coronaviridae, and the genus Coronavirus in systematic classification. Viruses of the genus Coronavirus are RNA viruses with an envelope and a linear single-stranded positive strand genome. They are a large class of viruses that exist widely in nature....

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/36G01N30/86G01N33/68
CPCG01N30/02G01N30/36G01N30/8679G01N33/6848Y02A50/30
Inventor 吴建国朱维罡吴伟根吴英萍李游江
Owner 浙江大学医学院附属第四医院
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