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Method for preparing plague attenuated live vaccine dry powder agent

A technology of attenuated vaccine and dry powder, which is applied in the directions of non-active ingredients medical preparations, medical preparations containing active ingredients, powder delivery, etc., can solve the problem of no relevant reports on the preparation of dry powder of live attenuated plague vaccine, etc. Conducive to storage and transportation, good water solubility, weak hygroscopic ability

Active Publication Date: 2020-06-30
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no relevant report on the dry powder preparation of live attenuated plague vaccine in domestic and foreign research

Method used

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  • Method for preparing plague attenuated live vaccine dry powder agent
  • Method for preparing plague attenuated live vaccine dry powder agent
  • Method for preparing plague attenuated live vaccine dry powder agent

Examples

Experimental program
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Embodiment 1

[0040] Embodiment 1, preparation of dry powder of plague attenuated vaccine

[0041] 1. Cultivation of Yersinia pestis

[0042] The tested strain was Yersinia pestis attenuated vaccine strain Yersinia pestis 201.

[0043] (1) Activation: Glycerol strains were inoculated in 20ml of BHI broth, cultured at 200rpm at 26°C for 36h (plateau stage, OD 600 = 2.5 or more). Do not store at 4°C and use directly.

[0044] (2) Pre-cultivation: Glycerol strains were inoculated in 20ml of BHI broth, and cultured at 200rpm at 26°C for 36h (plateau stage, OD 600 = 2.5 or more). Do not store at 4°C and use directly.

[0045] (3) Formal culture: 100-fold dilution, transfer to 20 ml of BHI broth, culture at 26° C. at 200 rpm until OD600 = 1.0 (10-12 hours). Then transfer to 200rpm at 37°C and cultivate until OD600=1.6-1.8 (3-4h). Transfer the third-generation bacterial solution to a shaker at 37°C at 200 rpm for 3 hours, when the bacterial cells are in the mid-logarithmic phase (OD 600 =1...

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Abstract

The invention discloses a method for preparing a plague attenuated live vaccine dry powder agent. The method for preparing the plague attenuated live vaccine dry powder agent comprises the following steps: mixing a Yersinia pestis biovar Microtus strain, freeze-drying protective additives (skimmed milk, trehalose, inositol, dextran-40, glycerinum and urea), storage protective agents (sodium glutamate and a thiourea group) and dispersants (leucine and poloxamer) with deionized water so as to obtain a mixed liquid with bacteria; and performing vacuum freeze drying. The plague attenuated live vaccine dry powder tablet solid prepared by using the method disclosed by the invention is low in water content, weak in water absorption capacity, beneficial to preservation and transportation and goodin water solubility, and possibility is provided for next steps of dry powdering preparation and dry powder aerosol administration of inhalable attenuated live vaccines.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing dry powder of live attenuated plague vaccine. Background technique [0002] Plague is a natural foci disease caused by Yersinia pestis, which is usually prevalent among rodents and can occasionally cause human epidemics. The spread of plague belongs to the international quarantine infectious disease, and it is also the first class A infectious disease stipulated in my country's "Infectious Disease Law". Our country has been closely monitoring the plague, especially the natural foci of the plague, to prevent the spread of the plague in our country. Plague has an acute onset, short course of disease, high mortality, and strong infectivity, which can easily cause large-scale transmission among the population. Especially septicemic plague and pneumonic plague, if left untreated, the case fatality rate is 30%-100%. The incubation period of plague is short, genera...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61K9/19A61K47/10A61K47/18A61K47/20A61K47/26A61K47/46A61P31/04
CPCA61K39/0291A61K47/183A61K47/10A61K47/26A61K47/20A61K47/18A61K47/46A61K9/19A61P31/04Y02A50/30
Inventor 周冬生杨文慧曹超越胡凌飞赵月峨孙岩松邱业峰熊小路杨慧盈殷喆焦俊于学东李越张丽丽
Owner ACADEMY OF MILITARY MEDICAL SCI
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