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Anti-pollution polypeptide, nerve electrode modified by anti-pollution polypeptide, modification method of nerve electrode and application of anti-pollution polypeptide

A peptide modification and anti-pollution technology, applied in the field of neurobiological materials and surface modification, can solve the problems of reducing protein adsorption resistance and limiting applications, and achieve the effects of enhanced interaction, guaranteed effectiveness, and increased adhesion

Pending Publication Date: 2020-07-24
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polyethylene glycol (PEG) is a traditional antifouling surface modification material, however, PEG reduces resistance to protein adsorption at temperatures above 35 °C, limiting its application in a wider range of real biological environments

Method used

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  • Anti-pollution polypeptide, nerve electrode modified by anti-pollution polypeptide, modification method of nerve electrode and application of anti-pollution polypeptide
  • Anti-pollution polypeptide, nerve electrode modified by anti-pollution polypeptide, modification method of nerve electrode and application of anti-pollution polypeptide
  • Anti-pollution polypeptide, nerve electrode modified by anti-pollution polypeptide, modification method of nerve electrode and application of anti-pollution polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] This embodiment provides an anti-pollution polypeptide.

[0065] The anti-pollution polypeptide Acetyl-C-PPPP-EKEKEK-IKVAV (hereinafter referred to as EK-IK for short) consists of four parts, such as figure 1 As shown, the bifunctional fragments EKEKEK and IKVAV are used to resist protein adsorption and increase neuronal adhesion, the connecting segment C is used to modify the anti-pollution polypeptide to the surface of the site through gold-sulfur bonds, and the supporting segment PPPP is used to bind the two functional fragments exposed on the outside of the electrode surface.

[0066] The synthesis and modification of the polypeptides were entrusted to Hefei Guopeptide Biotechnology Co., Ltd.

Embodiment 2

[0068] In this example, the anti-adsorption ability, hydrophilicity and adhesion ability to neurons of the gold surface modified with the anti-pollution polypeptide prepared in Example 1 were tested.

[0069] (1) Anti-adsorption ability test

[0070] By surface plasmon resonance (SPR), the gold surface modified by the anti-pollution polypeptide EK-IK was tested for 1mg / mL lysozyme (Lysozyme), fibrinogen (Fibrinogen), immunoglobulin G (Immunoglobulin G, IgG ) and 2mg / mL human serum albumin (human serum albumin, HSA), the anti-adsorption ability of several typical proteins in organisms, the specific results are as follows figure 2 shown.

[0071] Simultaneously, figure 2 The anti-pollution polypeptide EK-IK is also horizontally compared in the above, and the polypeptide containing only anti-pollution function (EKEKEKEPPPPC-Am, Am is to amidate the carboxyl group of the carbon-terminal cysteine ​​to balance the charge of the carbon-terminal, hereinafter referred to as The ad...

Embodiment 3

[0080] In this example, the anti-pollution polypeptide prepared in Example 1 is used to modify the surface sites of the flexible nerve electrode.

[0081] The flexible nerve electrode is a flexible nerve tassel electrode with 33 channels, and the specific operation steps are as follows:

[0082] (1) Pretreatment of the surface of the flexible nerve tassel electrode site

[0083] First place the flexible nerve tassel electrode in 0.5M ferric chloride, remove the aluminum sacrificial layer of the flexible nerve tassel electrode, and release the self-supporting flexible nerve tassel electrode; carefully place the nerve tassel electrode in the acetone solution to ensure that it is at the tip of the needle Immerse the electrode site area, sonicate for 30s, then quickly immerse it in isopropanol, sonicate for 30s, and then clean it with deionized water for 30s, wash away the residual acetone and isopropanol solution, and pass the three-step process The cleaning step removes non-spe...

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Abstract

The invention relates to an anti-pollution polypeptide, a nerve electrode modified by the anti-pollution polypeptide, a modification method of the nerve electrode and an application of the anti-pollution polypeptide. The anti-pollution polypeptide comprises a connecting section, a supporting section, an anti-protein adsorption section and a neuron adhesion section which are connected in sequence;and the anti-protein adsorption section comprises a zwitterionic polypeptide fragment. According to the anti-pollution polypeptide, through electrostatic interaction between charged side groups of thezwitterionic polypeptide fragment and water molecules, the adsorbability of the surface of the nerve electrode to common proteins such as lysozyme, fibrinogen, immune globulin G and human serum albumin is reduced; and meanwhile, the connecting section, the supporting section, the anti-protein adsorption section and the neuron adhesion section are matched with one another, so that the protein pollution resisting function of the polypeptide is guaranteed, the adhesion of the modified surface to neuronal cells is improved, and the long-term signal regulation and measurement stability of the neural electrode in a biological environment is effectively improved.

Description

technical field [0001] The invention belongs to the field of neurobiological materials and surface modification, and in particular relates to a neuroelectrode with anti-protein adsorption capability, in particular to an anti-pollution polypeptide, a neuroelectrode modified by the same, a modification method and application. Background technique [0002] The brain is the most complex and precise organ of our human body. Various functions of the human body are precisely controlled by the electrical activity of neurons. Therefore, it is necessary to effectively record or adjust the electrical activity of neurons and analyze it for a long time. An important foundation for the study of brain diseases. [0003] Nerve electrode is an important method to record the electrical activity of neurons. Its micromechanical multi-channel electrode array can be directly implanted in the body to realize the stimulation and recording functions of peripheral and central nervous system neurons. ...

Claims

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Application Information

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IPC IPC(8): C07K19/00A61N1/05A61B5/0478A61B5/00
CPCC07K14/00A61N1/0531A61B5/6868C07K2319/00A61B5/291
Inventor 方英邹宜旻王晋芬王琛
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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