Fetal cell capturing module, application method thereof, micro-fluidic chip for fetal cell capturing and application method of micro-fluidic chip for fetal cell capturing

Abstract

The invention relates to a fetal cell capturing module, an application method thereof, a micro-fluidic chip for fetal cell capturing and an application method of the micro-fluidic chip for fetal cellcapturing. The fetal cell capturing module comprises a cell capturing carrier and a distinguishing molecule for specific capturing of cells, and the distinguishing molecule is connected to the surfaceof the carrier through an organic coupler containing disulfide bonds. The surface of the micro-fluidic chip for fetal cell capturing is modified with the distinguishing molecule used for specific capturing of the fetal cells by means of the organic coupler containing disulfide bonds, and after the distinguishing molecule captures the cells, chemical chopping is performed on the disulfide bonds inthe organic coupler to realize the release of the cells. The capturing module, the application method thereof, the micro-fluidic chip and the application method of the micro-fluidic chip can realizefetal cell capturing of whole blood without being pretreated, the capturing rate is high, the cell loss is low, the cells are simple in release operation and accurate in action, so that the fetal cells can be efficiently and harmlessly released, and complete genomes can be analyzed.

Description

technical field

[0001] This application relates to the field of cell capture. It specifically relates to a fetal cell capture module, a microfluidic chip for fetal cell capture, and their use methods. Background technique

[0002] Improving reproductive health and preventing and controlling major birth defects is one of the important goals of Healthy China. The "Report on the Prevention and Control of Birth Defects in China" issued by the Ministry of Health in 2012 pointed out that the total incidence of birth defects in my country is about 5.6%, and the number of new birth defects is about 900,000 every year. After the implementation of the "universal two-child" policy, the number of elderly pregnant women has increased, and the prevention and control of birth defects is also facing more challenges. "Prenatal screening and diagnosis" is the most important means of preventing birth defects. Amniocentesis, chorionic villus biopsy, and umbilical cord blood aspiration, as th...

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