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Automatic detection card and detection method of multi-blood group system

An automatic detection and detection card technology, applied in biological testing, material inspection products, etc., can solve problems such as limiting application value, improving detection false negative rate, false positive rate, etc., to improve detection stability and accuracy, and reduce labor. Judgment error, the effect of preventing cross-contamination

Pending Publication Date: 2020-07-24
CHONGQING UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

The test card is easy to carry and the detection speed is fast, but it only detects the positive and negative ABO stereotypes. If there is a discrepancy between the positive and negative stereotypes of ABO, it will easily lead to detection errors
Chinese patent CN103389384B discloses a Miltenberger blood type antibody detection immunofluorescence chromatographic test strip and its detection method, which detects Miltenberger blood type quantitatively with fluorescence, but the fluorescence detection often needs to be equipped with large-scale professional instruments, which is limited in practical application. Applicable to areas with scarce resources and economically underdeveloped areas
Chinese patent CN107167616B provides a blood type detection method and device. After using multiple reaction chambers to coat different antibodies, the blood to be tested and the antibodies undergo an immune reaction to collect images, convert the color image into a grayscale image, and judge by the grayscale value Blood type results; although it provides certain support for automated detection, two different colors may have close gray values, resulting in errors in test results and increasing the false negative rate and false positive rate of detection; at the same time, it is impossible to identify whether it is a blood sample;
[0005] Most of the above-mentioned methods detect a single blood type or certain antigens in the blood group system, and cannot automatically and synchronously detect common blood types (such as Rh blood type, MNS blood type, etc.); conventional techniques take a long time to detect and require additional special equipment, which is not universal. Type detection method, automatic detection has not been fully realized, it is difficult to meet the use in emergency situations and economically backward areas, which greatly limits its application value

Method used

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  • Automatic detection card and detection method of multi-blood group system

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Human ABO blood type and Rh(C / c / D / E / e) blood type automatic detection

[0040] Use wax printing technology to build a three-dimensional detection card structure, such as Figure 1a ~ Figure 1d As shown, the sample application layer and the reaction layer are set separately, the sample application hole 1 is circular; The material is non-woven paper as an example, and the material of the filter pad 2 is whole blood separation membrane as an example.

[0041] The antibody pad 4 is pre-coated with 7 antibodies against A, B, C, c, D, E, and e, which are of IgM type; the quality control area is infiltrated with saline and then dried; the delay pad 5 is pre-coated with dextran As an example, they are all fixed after freeze-drying at -80°C; the material of the color-developing layer is nitrocellulose membrane as an example, and the color-developing area is 8 square areas, and the distribution corresponds to 7 test results of ABO and Rh blood types (number 1-7) and No. 8 qualit...

Embodiment 2

[0050] Human MNS, Kell, P, Kidd blood type automatic detection

[0051] Use origami technology to build a three-dimensional detection card structure, such as Figure 1a ~ Figure 1d As shown, the sample layer and the reaction layer are combined into one, and the sample hole 1 is square; the material of the reaction layer whole blood pad 3, antibody pad 4, delay pad 5, and antigen pad 6 is filter paper as an example, filter pad 2 The material is carbon paper as an example;

[0052] The antibody pad is pre-packed with 9 antibodies against M, N, S, s, K, k, P, Jka, and Jkb, which are mixed types of IgM and IgG, and are all fixed after vacuum drying at 30°C; the quality control area is infiltrated with ultrapure water After drying; the material of the color development layer is cellulose acetate film as an example, the color development area is 10 square areas, and the distribution corresponds to 9 test results (number 1-9) of MNS, Kell, P, Kidd blood types and No. 10 quality contr...

Embodiment 3

[0062] Human ABO positive and negative typing, Rh (C / c / D / E / e) blood type automatic detection

[0063] Use wax printing technology to build a three-dimensional detection card structure, such as Figure 1a ~ Figure 1d As shown, the sample application layer and the reaction layer are set separately, and the sample application hole 1 is circular; the material of the sample application layer is filter paper as an example, and the material of the reaction layer is whole blood pad 3, antibody pad 4, delay pad 5, and antigen pad 6. Taking non-woven fabric as an example, the material of filter pad 2 is example glass fiber;

[0064] The antibody pad is pre-coated with 7 antibodies against A, B, C, c, D, E, and e, which are of IgM type; the quality control area is infiltrated with saline and then dried; the delay pad is pre-coated with pectin as an example. All were fixed after freeze-drying at -80°C; ABO blood type A1, A2, B, and O red blood cells were added to the antigen pad to detect...

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Abstract

The invention relates to an automatic detection card and detection method of a multi-blood group system. The automatic detection card comprises four parts, namely a sample addition layer, a reaction layer, a developing layer and an identification layer from top to bottom, wherein a sample addition hole is formed in the sample addition layer, a full-blood pad or a filter pad for filtering red bloodcells from full blood is arranged on the reaction layer, the bottom of the sample addition hole is connected with the full-blood pad or the filter pad, a tail end of the full-blood pad is connected with a plurality of antibody pads pre-coated with blood group antibodies, a tail end of the filter pad is connected with an antigen pad, pre-coated with a blood group antigen, through a delay pad, thereaction layer is separately provided with a quality control strip, a plurality of developing regions connected with tail end(s) of the antibody pads or the antigen pad are arranged on the developinglayer, and the identification layer is reversely connected with the developing layer. In the automatic detection card, blood group detection and a coding technology are combined, multiple blood groupscan be rapidly detected, automatic identification of multiple blood groups is achieved, artificial judgment errors are reduced, the detection efficiency is improved, special equipment is not needed,and rapid, accurate, automatic and portable detection of the blood groups is achieved.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnosis of clinical examination, and relates to an automatic detection card for multiple blood type systems and a detection method. Background technique [0002] Blood type refers to the type of surface antigens of blood components, and usually refers to the typing of specific antigens on the red blood cell (RBC) membrane. More than 35 blood group systems have been discovered and recognized by the International Society of Blood Transfusion, among which the ABO blood group system and the Rh blood group system are most closely related to human blood transfusion, and other systems such as MNS and Kell are also closely related to certain diseases. Blood typing refers to the technology of identifying cell surface antigens in blood components, which is mainly used in clinical blood transfusion, organ transplantation, hemolytic disease of newborns, etc. Accurate blood type identification is very impo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80
CPCG01N33/80G01N33/54391G01N33/531G01N33/558
Inventor 罗阳张洪
Owner CHONGQING UNIV
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