Copper composite based intelligent nanometer material and preparation method and antitumor application thereof

A technology of nanomaterials and composites, applied in antitumor drugs, wave energy or particle radiation treatment materials, drug combinations, etc., can solve the problems of limiting DSF antitumor effects, incompletely clear biodistribution and dynamic equilibrium mechanisms, carcinogenesis, etc. problems, achieve the effect of inhibiting proliferation and metastasis, and improving tumor killing ability

Inactive Publication Date: 2020-07-31
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, there are many problems in the treatment of cancer with DSF, such as: (1) The proper content of Cu ions is the prerequisite for the formation of CuET, but the biodistribution and dynamic balance mechanism of intrinsic Cu in the body are not fully understood, and Cu ions in the tumor microenvironment Insufficiency will also limit the anti-tumor effect of DSF; (2) Copper is an essential trace element for cells, based on the formation mechanism of CuET, it may form CuET in normal cells and tissues, resulting in unexpected side effects and even toxicity
Therefore, increasing the selective tumor delivery of DSF is the key to therapy; (3) Given that, so far, the threshold for regulating copper metabolism between normal cells and tumor cells is not clear, and excess copper may cause other carcinogenesis, So supplementing with extra copper in patients who are not deficient in copper can cause more problems

Method used

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  • Copper composite based intelligent nanometer material and preparation method and antitumor application thereof
  • Copper composite based intelligent nanometer material and preparation method and antitumor application thereof
  • Copper composite based intelligent nanometer material and preparation method and antitumor application thereof

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Experimental program
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Embodiment 1

[0043] The synthesis of embodiment 1CuET

[0044] Water-soluble divalent copper salt and sodium diethyldithiocarbamate were dissolved in deionized water respectively, and the metal ion solution and sodium diethyldithiocarbamate solution were mixed at a molar ratio of 1:2 to form a precipitate immediately , the precipitate was collected by centrifugation and dissolved in chloroform again; then, ethanol was slowly added above the chloroform solution, and diffused at room temperature for a week to obtain dark brown CuET, which was collected by centrifugation.

Embodiment 2

[0045] The synthesis of embodiment 2CuET / DIR NPs

[0046]Dodecanoic acid and octadecanoic acid (mass ratio 4:1) were dissolved in methanol to obtain PCM solution; phospholipids and distearyl fatty ethanolamine-polyethylene glycol were dissolved in 4% ethanol aqueous solution according to a certain mass ratio , to obtain a phospholipid solution; take a certain volume of the phospholipid solution and heat it to about 50 ° C; weigh a certain amount of CuET crystals and DIR dyes and dissolve them in an organic solvent; mix the PCM solution, CuET solution and DIR solution evenly, and then add it dropwise to React in the preheated phospholipid solution for a certain period of time, then transfer the reaction solution to a centrifuge tube, vortex vigorously for 3 minutes, and place it in an ice-water bath to cool for 2 minutes; then, transfer the above cloudy solution to a beaker and stir at room temperature until the solution temperature returns to room temperature . After the reac...

Embodiment 3

[0047] Particle size distribution and Zeta potential characterization of embodiment 3 CuET / DIR NPs

[0048] The prepared CuET / DIR NPs were analyzed for particle size and potential by a particle size detector, and the results were as follows: figure 1 As shown in A and 1B, the hydrated particle size of the nanomaterial is about 123nm, and the Zeta potential is -20.8mV.

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Abstract

The invention discloses a copper composite based intelligent nanometer material and a preparation method and antitumor application thereof, and belongs to the fields of a nanometer material and preparation thereof. A copper composite is synthesized firstly, the copper composite and near infrared fluorescent dyes are loaded to an organic phase change material together, and a light control intelligent nanometer material being uniform to disperse and good in light heat efficiency can be prepared. The nanometer material can cause DNA injury and apoptosis through light control target cell nucleus,an EMT process is restrained, the transfer ability of tumor cells is destroyed, and the copper composite based intelligent nanometer material is hopeful to be used for antitumor research.

Description

technical field [0001] The invention relates to the field of nanomaterials and their preparation, in particular to a copper composite-based smart nanomaterial, its preparation method and anti-tumor application. Background technique [0002] High cost, long research time and low probability of success greatly limit the clinical application of new drugs. To address these issues, repurposing drugs that have been clinically approved for other diseases to treat cancer has attracted extensive attention in recent years. [0003] Disulfiram (DSF) is an alcohol cessation drug approved by the US FDA. Its function is to inhibit the activity of acetaldehyde dehydrogenase (ALDH), so that the alcohol metabolite acetaldehyde accumulates in the body, thereby achieving the purpose of alcohol abstinence. In recent years, preclinical studies have shown that DSF can inhibit the occurrence and metastasis of tumors by inhibiting protease activity, promoting apoptosis, depleting tumor stem cells ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/51A61K47/24A61K33/34A61P35/00A61P35/04
CPCA61K9/0009A61K9/5123A61K33/34A61K41/00A61P35/00A61P35/04
Inventor 刘思金任丽婷冯文雅徐明马娟
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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