Materials and methods for repairing concrete cracks based on mineralized deposition of facultative aerobic bacteria
A facultative aerobic and repairing material technology, applied in building maintenance, construction, building construction, etc., to achieve the effects of enhanced stability, easy preservation, and strong resistance to environmental impact
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[0038] c. Preparation of selective culture medium; aerobic microorganism selective culture medium: ultrapure water 1L, sodium lactate 0.85g, ammonium chloride 0.02g, trace element solution 10mL; anaerobic microorganism selective culture medium: ultrapure water 1L, sodium lactate 0.85g, sodium nitrate 0.03g, trace element solution 10mL;
[0039] d. Facultative aerobic mixed bacteria culture; the facultative aerobic mixed bacteria culture method is divided into two stages, that is, the enhancement of aerobic characteristics and the enhancement of anaerobic characteristics; aerobic cultivation is carried out first, and then anaerobic cultivation is carried out, which is a cycle , continuous culture for 10 cycles;
[0040] Characteristic enhancement process: Add 10mL of sludge mixture and 100mL of aerobic microbial selection culture solution into a 300mL conical flask at a ratio of 1:10, and use Na 3 PO 4 Adjust the pH to 11, cover the mouth of the bottle with sterile cotton gau...
Embodiment 1
[0050] In this embodiment, the 1mm wide crack at the water seepage of the building floor is repaired.
[0051] The required materials are prepared by mixing solution A and solution B at a ratio of 1:1; among them,
[0052] Solution A: 1L ultrapure water, 9g tryptone, 5g yeast extract, 10g sodium chloride, adjust pH to 7.0 with 5mol / L NaOH, facultative aerobic mixed bacteria 1.6×10 7 a / L;
[0053] Solution B: 1L ultrapure water, 14g calcium lactate, 14g calcium nitrate, 4000g standard sand;
[0054] The facultative aerobic mixed bacteria used in the solution A is obtained by the following method:
[0055] a. Select the activated sludge of the sewage treatment plant and the garden mud as separate inoculation sources; the garden mud refers to the soil in the park; after the activated sludge of the sewage treatment plant and the garden mud are mixed, distilled water is added to prepare a sludge mixture;
[0056] b. Preparation of trace element solution, ultrapure water 1L, vita...
Embodiment 2
[0070] In this embodiment, the 1.4mm wide crack at the seepage of the building wall is repaired.
[0071] The required materials are prepared by mixing solution A and solution B at a ratio of 1:1; among them,
[0072] Solution A: 1L ultrapure water, 11g tryptone, 6g yeast extract, 11g sodium chloride, adjust pH to 7.0 with 5mol / L NaOH, facultative aerobic mixed bacteria 2.0×10 7 a / L;
[0073] Solution B: 1L ultrapure water, 15g calcium lactate, 15g calcium nitrate, 4700g standard sand;
[0074] The facultative aerobic mixed bacteria used in the solution A is obtained by the following method:
[0075] a. Select the activated sludge of the sewage treatment plant and the garden mud as separate inoculation sources; the garden mud refers to the soil in the park; after the activated sludge of the sewage treatment plant and the garden mud are mixed, distilled water is added to prepare a sludge mixture;
[0076] b. Preparation of trace element solution, ultrapure water 1L, vitamin ...
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