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A bioremediation technology for eutrophic water body

A bioremediation and eutrophication technology, which is applied in the field of habitat restoration, can solve the problems of affecting photosynthesis of plants in water, reducing water transparency, and supersaturation of dissolved oxygen, so as to improve photosynthesis efficiency, increase inhibition rate, and increase transcription level. Effect

Active Publication Date: 2022-04-12
ZHEJIANG MARICULTURE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Eutrophication will affect the water quality of the water body and cause the transparency of the water to decrease, making it difficult for sunlight to penetrate the water layer, thereby affecting the photosynthesis of plants in the water, and may cause a supersaturated state of dissolved oxygen

Method used

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  • A bioremediation technology for eutrophic water body
  • A bioremediation technology for eutrophic water body
  • A bioremediation technology for eutrophic water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. A preparation method of sustained-release microspheres, comprising:

[0037] 1) Dissolve 23g of stevioside and 15g of (2R,3R)-dihydroquercetin in 1L of sterile seawater to obtain a mixed solution of stevioside and (2R,3R)-dihydroquercetin, and take 50g of alginic acid Sodium powder was dissolved in 1L of sterile seawater to obtain a viscous solution of sodium alginate, and after mixing the two evenly, a material colloid solution was obtained;

[0038] 2) Take 100g of calcium chloride and 40g of chitosan powder and dissolve them in 5L of 1.2% (m / v) acetic acid solution to make a fixative solution;

[0039] 3) Drop the material colloid solution in 1) into the fixative solution in 2) drop by drop. After the dropwise addition, let it stand for 50 minutes to obtain slow-release microspheres. Remove the fixative solution and wash the slow-release microspheres with deionized water for 3 times. Freeze dry under vacuum.

[0040] 2. A preparation method for immobilized composi...

Embodiment 2

[0052] A kind of method for asparagus enrichment culture:

[0053] Pro culture medium composition: N: NaNO 3 3.5g; P: NaH 2 PO 4 0.2723g; Fe–Solution: Fe(NH 4 ) 2 (SO 4 ) 2 •6H 2 O 0.17525g, Na 2 EDTA 0.165g; Metal Solution: Na 2 EDTA 0.25g,H 3 BO 3 0.285g, FeCl 3 •6H 2 O 0.0125g, MnCl 2 • 4H 2 O 0.0365g, ZnSO 4 •7H 2 O 0.0055g, CoCl 2 •6H 2 O 0.001g; H 2 O640mL.

[0054] Preparation of culture solution A: Dilute the Pro culture solution with 100 times the volume of seawater, add 12mg / L zingerone, and set HCO 3 - It is 35mg / L.

[0055] The seawater used for the cultivation of asparagus was taken from the vicinity of Tuandao Wharf in Jiaozhou Bay, with a salinity of 32 and a pH of 8.10. After filtering through a filter membrane with a pore size of 0.45 µm, sterilize with moist heat for 20 minutes, and let it stand for 24 hours to balance.

[0056] After the asparagus used in the experiment was cultured in the collected seawater for 10 days, healthy al...

Embodiment 3

[0058] No zingerone was added when the culture solution A was prepared, and the rest was exactly the same as in Example 2.

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Abstract

The invention provides a bioremediation technology for eutrophic water bodies, which belongs to the field of habitat restoration, and includes adding zingerone at a ratio of 11-13mg / L seawater, and adding pre-cultured 8 at a ratio of 3-4.8g / L seawater. For asparagus that grows well for ‑10 days, add immobilized composite bacteria at a ratio of 300‑500g / L seawater. After 2‑3 days, add a regulator at a ratio of 70‑120g / L seawater. The method provided by the invention can stimulate the expression of asparagus carbonic anhydrase gene, improve the activity of carbonic anhydrase, improve the efficiency of photosynthesis, enhance the ability to compete with red tide algae for nutrient salt, and improve the allelopathic effect on red tide algae at the same time. Effectively improve water quality.

Description

technical field [0001] The invention belongs to the field of habitat restoration, and in particular relates to a bioremediation technology of eutrophic water body. Background technique [0002] Eutrophication of water body refers to that under the influence of human activities, a large amount of nutrients such as nitrogen and phosphorus required by organisms enter slow-flowing water bodies such as lakes, estuaries, and bays, causing algae and other plankton to multiply rapidly, and the dissolved oxygen in water bodies decreases. Deterioration of water quality, massive death of fish and other organisms. In only the last few decades, many previously oligotrophic estuaries and offshore waters have undergone a dramatic transformation to become mesotrophic and eutrophic. Eutrophication will affect the water quality of the water body, and will reduce the transparency of the water, making it difficult for sunlight to penetrate the water layer, thereby affecting the photosynthesis ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/63C02F103/08
CPCC02F3/34C02F3/32A01G33/00C02F2103/08Y02A40/80
Inventor 彭欣张华伟刘俊峰薛峰
Owner ZHEJIANG MARICULTURE RES INST
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