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Cartilage repair material containing translationally controlled tumor protein as well as preparation method and application thereof

A tumor protein and cartilage repair technology, applied in the field of cells, can solve the problems of changing the state and vitality of chondrocytes, aseptic inflammation, and the cumbersome extraction process of hyaluronic acid, so as to promote the proliferation of chondrocytes, promote the repair of cartilage damage, and achieve good results. Effects of histocompatibility and cell adsorption

Inactive Publication Date: 2020-08-21
广州市红十字会医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Transforming growth factor-β3 (TGF-β3) and bone morphogenetic protein-6 (BMP-6), as the most common growth factors, have the characteristics of rapid onset and significant short-term curative effect, but TGF-β3 and BMP-6 have the characteristics of promoting Differentiation will change the state and vitality of chondrocytes to varying degrees, and cannot repair cartilage damage well
Hormone has a rapid onset effect, but because of its large side effects, the long-term treatment effect is not good
Hyaluronic acid is widely used clinically, but hyaluronic acid has the disadvantages of cumbersome extraction process, high price, and cumbersome preparation steps of composite collagen hydrogel
In addition, synthetic degradable polymers have the disadvantages of poor hydrophilicity, weak cell adsorption and may cause aseptic inflammation

Method used

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  • Cartilage repair material containing translationally controlled tumor protein as well as preparation method and application thereof
  • Cartilage repair material containing translationally controlled tumor protein as well as preparation method and application thereof
  • Cartilage repair material containing translationally controlled tumor protein as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Construction of TCTP-sodium alginate-collagen sponge-chondrocyte 3D complex

[0030] 1. Isolation of primary articular chondrocytes from neonatal SD rats

[0031] Three newborn SD rats were taken, anesthetized and killed in a sterile environment, the femoral head and tibia were separated with a sterile scalpel blade and ophthalmic forceps, the soft tissue was removed, and the knee articular cartilage at the separated place was put into 1×PBS buffer and washed Twice; transfer the separated articular cartilage into type I collagenase (0.7mg / mL) digestion solution, shake and digest in a constant temperature water bath at 37°C for 30 minutes, after that, discard the viscous solution. Put the articular cartilage that has digested muscle tissue into the digestive solution containing collagenase type I (0.7mg / mL) and collagenase type D (3mg / mL), blow off the tissue pieces, digest repeatedly, and shake in a constant temperature water tank at 37°C Digest for 4-6 hours...

Embodiment 2

[0039] Example 2 verifies the repair effect of the chondrocyte 3D complex of the present invention on damaged articular cartilage

[0040] 1. Establishment of 6-week-old SD rat articular cartilage injury model

[0041] Under sterile conditions, 6-week-old SD rats were anesthetized with xylazine (5 mg / kg) and anesthesia chloramine (40 mg / kg). Under a stereo microscope, the skin of the right hind limb of the rat was cut along the medial edge of the patella , revealing the quadriceps, patella, and patellar tendon. Incision was made from the medial border of the patella (0.5 cm of soft tissue was reserved for suturing) to the tibia. Be careful not to strip too much medial or lateral to prevent damage to the medial collateral ligament or patellar ligament, which may cause serious complications. The infrapatellar fat pad and other soft tissues beneath the patella and patellar ligament are then dissected to expose the medial and lateral femoral condyles. At this time, the smooth su...

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Abstract

The invention provides a preparation method of a cartilage repair material containing translationally controlled tumor protein (TCTP) and application of the cartilage repair material in treating articular cartilage injury. The cartilage repair material constructed by the invention contains TCTP, so that the cartilage repair material has a strong cartilage cell proliferation promoting effect; the cartilage repair material has a 3D complex structure, the in-vivo survival microenvironment of cartilage cells is well simulated, the TCTP-containing cartilage repair material provides a suitable microenvironment for the growth and metabolism of the cartilage cells, and the rapid proliferation of the cartilage cells is ensured. According to the repair material disclosed by the invention, collagen sponge and sodium alginate are combined to serve as a scaffold, so that the cartilage repair material has the characteristics of bioabsorbability and no toxicity, has good tissue intermiscibility and cell adsorbability, and has a remarkable promoting effect on cartilage injury repair.

Description

technical field [0001] The invention belongs to the field of cell technology, and in particular relates to a cartilage repair material containing a translation-regulating tumor protein, a preparation method and an application. Background technique [0002] Articular cartilage injury is a common and frequently-occurring disease that is more common in weight-bearing joints such as the knee joint, often causing joint swelling, pain, and deformity. At present, there is no ideal treatment method. Cartilage tissue engineering is an emerging treatment method for the treatment of articular cartilage injury. The three elements of cartilage tissue engineering include: cells, scaffolds and growth factors. Currently, cell types include: stem cells and various differentiated cells; growth factors include: hormones and various cell growth factors; commonly used cell scaffolds include: artificially synthesized degradable polymers and hyaluronic acid scaffolds. [0003] Transforming growth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/24A61L27/38A61L27/50A61L27/54A61L27/58
CPCA61L27/20A61L27/24A61L27/3817A61L27/3852A61L27/50A61L27/54A61L27/58A61L2300/252A61L2300/412A61L2430/06C08L5/04
Inventor 王鹏珍熊喜峰张金丽殷文慧刘志河张少衡
Owner 广州市红十字会医院
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