Lactic acid bacteria compound preparation for fertilizing saline-alkali soil and preparation method thereof
A compound preparation and lactic acid bacteria technology, applied in the field of soil conditioning, can solve the problems of insignificant terrain improvement effect, consumption of human, material and financial resources, and high improvement costs, and achieve good application value, accelerate soil ripening, and promote the formation of humus.
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Embodiment 1
[0028] A lactic acid bacteria compound preparation for fertilizing saline-alkali land, prepared according to the following steps:
[0029] (1) Inoculate Lactobacillus plantarum, Lactobacillus rhamnosus, and Lactobacillus bulgaricus in MRS medium respectively, culture at a constant temperature of 35-37°C for 18-24 hours, and subculture 2-3 times in a cycle to obtain bacteria The culture is then put into a fermenter, and the secondary fermentation is carried out at 28°C according to the inoculum size of 5% for 48 hours, the sludge is collected by centrifugation, and the corresponding bacterial powder is obtained by vacuum freeze-drying;
[0030] (2) Inoculate the yeast in YPD liquid medium, culture at 25-30°C and 150rpm at a constant temperature for 18-24 hours, then put it into a fermenter, and carry out the second stage at 28°C according to the inoculum size of 5%. Ferment for 48 hours, collect the bacteria sludge by centrifugation, and obtain yeast powder by vacuum freeze-dry...
Embodiment 2
[0040] A lactic acid bacteria compound preparation for fertilizing saline-alkali land, prepared according to the steps of Example 1, wherein in step (4) Lactobacillus plantarum powder, Lactobacillus rhamnosus powder, Lactobacillus bulgaricus powder, yeast powder and Bacillus subtilis The powder is mixed evenly according to the mass ratio of 2:0.5:1.5:1.3:0.8 to prepare the lactic acid bacteria compound preparation.
Embodiment 3
[0042] A lactic acid bacteria compound preparation for fertilizing saline-alkali land, prepared according to the steps of Example 1, wherein in step (4) Lactobacillus plantarum powder, Lactobacillus rhamnosus powder, Lactobacillus bulgaricus powder, yeast powder and Bacillus subtilis The powder is mixed evenly according to the mass ratio of 2:1:1.2:1:0.5 to prepare the lactic acid bacteria compound preparation.
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