Lactic acid bacteria compound preparation for fertilizing saline-alkali soil and preparation method thereof
A compound preparation and lactic acid bacteria technology, applied in the field of soil conditioning, can solve the problems of insignificant terrain improvement effect, consumption of human, material and financial resources, and high improvement costs, and achieve good application value, accelerate soil ripening, and promote the formation of humus.
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[0027] Example 1
[0028] A lactic acid bacteria compound preparation for fertilization in saline-alkali soil is prepared according to the following steps:
[0029] (1) Inoculate Lactobacillus plantarum, Lactobacillus rhamnosus, and Lactobacillus bulgaricus respectively in MRS medium, culture at 35-37℃ for 18-24 hours, and subculture 2-3 times to obtain the bacteria The culture was then put into a fermentor, and subjected to secondary fermentation at 28°C at 5% inoculum for 48 hours, centrifuged to collect the bacterial sludge, and vacuum freeze-dried to obtain the corresponding bacterial powder;
[0030] (2) Inoculate the yeast in YPD liquid medium, culture it at a constant temperature at 25-30℃, 150rpm for 18-24 hours, then put it into the fermenter, and carry out the second stage at 28℃ according to the inoculation amount of 5% Fermentation for 48h, centrifugation to collect the sludge, and vacuum freeze-drying to obtain yeast powder;
[0031] (3) Inoculate the original species of...
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[0039] Example 2
[0040] A lactic acid bacteria compound preparation for fertilization in saline-alkali soil, prepared according to the steps of Example 1, wherein in step (4), Lactobacillus plantarum powder, Lactobacillus rhamnosus powder, Lactobacillus bulgaricus powder, yeast powder and Bacillus subtilis The powder is evenly mixed in a mass ratio of 2:0.5:1.5:1.3:0.8 to prepare a lactic acid bacteria composite preparation.
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[0041] Example 3
[0042] A lactic acid bacteria compound preparation for fertilization in saline-alkali soil, prepared according to the steps of Example 1, wherein in step (4), Lactobacillus plantarum powder, Lactobacillus rhamnosus powder, Lactobacillus bulgaricus powder, yeast powder and Bacillus subtilis The powder is evenly mixed in a mass ratio of 2:1:1.2:1:0.5 to prepare a lactic acid bacteria composite preparation.
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