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Nano-composite with encapsulated antibiotic and externally-connected antibacterial peptide, and preparation method of nano-composite

A nano-composite, antimicrobial peptide technology, applied in the direction of antibacterial drugs, active ingredients of heterocyclic compounds, drug delivery, etc., can solve the problems of digestion and degradation of antimicrobial peptides, increase antimicrobial activity, prolong half-life, and reduce the incidence of drug-resistant strains. produced effect

Pending Publication Date: 2020-08-28
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally composed of 12-50 amino acids, it has various biological functions such as antibacterial, antiviral, antiparasitic, anticancer, etc. It is considered to be one of the best substitutes for antibiotics, but a single antibacterial peptide is easily absorbed by the body Enzymatic digestion and degradation

Method used

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  • Nano-composite with encapsulated antibiotic and externally-connected antibacterial peptide, and preparation method of nano-composite
  • Nano-composite with encapsulated antibiotic and externally-connected antibacterial peptide, and preparation method of nano-composite
  • Nano-composite with encapsulated antibiotic and externally-connected antibacterial peptide, and preparation method of nano-composite

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Embodiment 1

[0028] The antimicrobial peptide nanocomposite containing antibiotics and external linking antimicrobial peptides of this embodiment is composed of phospholipid polymer nanoparticles and antimicrobial peptides. The phospholipid polymer nanoparticles are composed of soybean lecithin and two hard Fatty acylphosphatidylethanolamine-polyethylene glycol-NH 2 The shell is formed by encapsulating antibiotics and polylactic acid-glycolic acid copolymer; the particle size of the antibiotic-incorporated external antimicrobial peptide nanocomposite is 0.3μm-13μm; the particle size of the phospholipid polymer nanoparticles is 0.05μm~ 1 μm; the antibiotic used in this example is ampicillin, commercially available; the antimicrobial peptide used is melittin MPX, commercially available.

[0029] This embodiment also provides a method for preparing the above-mentioned antibiotic-encapsulated and externally linked antimicrobial peptide nanocomposite, the method is:

[0030] S1, indocyanine green, s...

Embodiment 2

[0060] The antimicrobial peptide nanocomposite containing antibiotics and external linking antimicrobial peptides of this embodiment is composed of phospholipid polymer nanoparticles and antimicrobial peptides. The phospholipid polymer nanoparticles are composed of soybean lecithin and two hard Fatty acylphosphatidylethanolamine-polyethylene glycol-NH 2 The shell is formed by encapsulating antibiotics and polylactic acid-glycolic acid copolymer; the particle size of the antibiotic-incorporated external antimicrobial peptide nanocomposite is 0.3μm-13μm; the particle size of the phospholipid polymer nanoparticles is 0.05μm~ 1 μm; the antibiotic used in this example is ofloxacin, commercially available; the antibacterial peptide used is BSN37, commercially available.

[0061] This embodiment also provides a method for preparing the above-mentioned antibiotic-encapsulated and externally linked antimicrobial peptide nanocomposite, the method is:

[0062] S1, indocyanine green, soybean p...

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Abstract

The invention provides a nano-composite with an encapsulated antibiotic and an externally-connected antibacterial peptide. The nano-composite is formed by externally connecting a phospholipid polymernanoparticle with an antibacterial peptide. The invention also provides a preparation method of the nano-composite. The preparation method comprises the following steps: the antibacterial peptide is dissolved in deionized water, and then suspended in a 2-(N-morpholine)ethanesulfonic acid buffer solution, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and N-hydroxysuccinimide are added, and therebya mixture c is obtained; a prepared concentrated solution of the phospholipid polymer nanoparticle is added into the mixture c, a 20 x phosphate buffer salt solution is added, dialyzing is performed after shaking, concentrating is performed by absorbing water with a polyethylene glycol powder, and thereby a concentrated solution of the nano-composite with an encapsulated antibiotic and an externally-connected antibacterial peptide is obtained; and the nano-composite with an encapsulated antibiotic and an externally-connected antibacterial peptide is dispersed and stored in a 1 x phosphate buffer salt solution. The nano-composite with an encapsulated antibiotic and an externally-connected antibacterial peptide can improve the antibacterial activity of a drug, reduces the generation of drug-resistant strains, and has antibacterial effects on respiratory system pathogenic bacteria, skin mucous membrane infectious bacteria and digestive system pathogenic bacteria.

Description

Technical field [0001] The invention belongs to the technical field of antibacterial drugs, and specifically relates to a nanocomposite containing antibiotics and externally linked antibacterial peptides and a preparation method thereof. Background technique [0002] Due to the long-term irrational use of antibiotics, the problems of antibiotic residues in animal food and the increase of bacterial resistance have become increasingly prominent, seriously threatening human health and the healthy development of animal husbandry. Therefore, there is an urgent need to find new alternatives to antibiotics. [0003] Antimicrobial peptides are a class of small molecule active peptides encoded by host genes, which can resist the invasion of pathogenic microorganisms and are an important part of the innate immune system. Generally composed of 12-50 amino acids, it has various biological functions such as anti-bacterial, anti-viral, anti-parasitic, and anti-cancer. It is considered to be one...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K9/51A61K31/43A61K38/17A61K45/06A61K47/34A61K47/24A61P31/04
CPCA61K47/6935A61K9/5146A61K9/5153A61K45/06A61K38/1767A61K31/43A61P31/04A61K2300/00
Inventor 王磊刘善芹赵雪芹夏小静张守平赵娅娅徐彦召孙亚伟杭柏林王彦华白跃宇胡建和
Owner HENAN INST OF SCI & TECH