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A kind of method that utilizes mud-water mixed solution to prepare microbial flocculant

A technology of microbial flocculant and mud-water mixture, which is applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problems of low yield and low effective content of flocculant, and achieve Improve the yield, use a large amount of enzyme preparation, and prolong the effect of enzyme action time

Active Publication Date: 2022-07-01
河南护理职业学院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a method for preparing microbial flocculant by using mud-water mixture, which solves the problems of low yield and low effective content of flocculant in the preparation of flocculant by existing sludge

Method used

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  • A kind of method that utilizes mud-water mixed solution to prepare microbial flocculant
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  • A kind of method that utilizes mud-water mixed solution to prepare microbial flocculant

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Effect test

Embodiment 1-7

[0039] A method for preparing a microbial flocculant utilizing a mud-water mixed solution, comprising the following steps:

[0040](1) Take the mud-water mixture at the end of the aeration tank of the sewage treatment plant, the sludge concentration in the mud-water mixture is 3g / L, then add protease and cellulase, and the enzyme activity of the protease is 300,000 U / g, The enzymatic activity of the cellulase is 100,000 U / g, the addition amount of the protease is 0.01-0.03%, the addition amount of the lipase is 0.005-0.01%, and the reaction is carried out at 37°C for 2 hours; The amount of cellulase added is based on the weight of the sludge in the mud-water mixture,

[0041] (2) carry out high-voltage electrostatic precipitation to the suspension, collect the precipitate, and set aside for use. The high-voltage electrostatic precipitation process in the step (2) is as follows: at a voltage of 35KV, electrostatically treating for 20min, and finally standing for 3h, removing th...

Embodiment 8-13

[0048] A method for preparing a microbial flocculant utilizing a mud-water mixed solution, comprising the following steps:

[0049] (1) Take the mud-water mixture at the end of the aeration tank of the sewage treatment plant, the sludge concentration in the mud-water mixture is 4g / L, then add protease and cellulase, and the enzyme activity of the protease is 300,000 U / g, The enzymatic activity of the cellulase is 100,000 U / g, the addition amount of the protease is 0.02%, the addition amount of the lipase is 0.005%, and the reaction is carried out at 30°C for 3 hours; The amount added is based on the weight of the sludge in the mud-water mixture,

[0050] (2) carry out high-voltage electrostatic precipitation to the suspension, collect the precipitate, and set aside for use. The high-voltage electrostatic precipitation process in the step (2) is: at a voltage of 30-50KV, electrostatically treating for 20-30min, and finally standing for 2h, Remove the supernatant and collect th...

Embodiment 13

[0053] In Example 13, instead of using high-voltage electrostatic precipitation, the method of natural gravity sedimentation was adopted, and the sediment was naturally settled for 12 hours, and then the sludge was collected.

[0054] The high-voltage electrostatic parameters and flocculation effects added with Examples 8-16 are shown in the following table.

[0055]

[0056] It can be seen from the above table that the high-voltage electrostatic parameters have an important influence on the prepared flocculant. If the voltage is too low or too high, the precipitation of proteins, polysaccharides, cellulose and other substances in the mud-water mixture will be incomplete or decomposed and deformed, causing it to flocculate. In particular, the high voltage has more serious damage to the components in the mud-water mixture, so that the flocculation ability of kaolin and sewage is lower than the flocculation effect without high-voltage electrostatic.

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Abstract

The invention relates to a method for preparing a microbial flocculant by using a mud-water mixed solution, comprising the following steps: (1) taking the mud-water mixed solution at the end of an aeration tank of a sewage treatment plant, then adding protease and cellulase, and reacting at 30-37° C. for 2 ‑3h; (2) carry out high-voltage electrostatic precipitation to the suspension, collect the precipitate, and use it for subsequent use; (3) the collected precipitate is prepared into a suspension by deionized water, and is subjected to ultrasonic crushing in an ice-bath environment; ( 4) After crushing, centrifuge to obtain a supernatant, which is mixed with pre-cooled ethanol or acetone, centrifuged to collect the precipitate, and freeze-dried to obtain a biological flocculant. The invention adopts enzymatic hydrolysis crushing, electrostatic precipitation, ultrasonic-alkaline-enzyme crushing and precipitation, and the yield of the prepared microbial flocculant is high, reaching 80-100 mg / g sludge, and the addition amount is 1 / 5000-1 / 8000, which is suitable for The flocculation rate of urban sewage reaches more than 98%.

Description

technical field [0001] The invention relates to a method for preparing a microbial flocculant by utilizing a mud-water mixed solution, and belongs to the field of biological flocculants. Background technique [0002] Microbial flocculant is a new type of flocculant obtained by extraction, separation and purification from microorganisms or their secretions using modern biotechnology. This flocculant not only has good flocculation and sedimentation performance, but also is safe, non-toxic, biodegradable, and will not cause damage to the ecological environment. At the same time, there are many types of microorganisms with flocculation performance, and they grow rapidly, which is easy to achieve industrialization through bioengineering methods. production. Glycoprotein, polysaccharide, protein, cellulose and other macromolecular compounds constitute its main active ingredients. The macromolecular polymer causes the suspended particles to flocculate and precipitate through the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34
CPCC02F3/34
Inventor 赵亚灿靳海科
Owner 河南护理职业学院
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