Chemiluminescent cleaning solution and its preparation method and application
A technology of chemiluminescence and cleaning solution, applied in chemical instruments and methods, detergent compositions, detergent compounding agents, etc., can solve problems such as single preservatives, jumping values, inaccurate detection results, etc., to improve detection accuracy And precision, prevent jumping value phenomenon, good anti-corrosion effect
Active Publication Date: 2021-09-21
ZHUHAI LIVZON DIAGNOSTICS
View PDF8 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
This is due to the chemiluminescence reagent of the alkaline phosphatase system. The principle of luminescence is that alkaline phosphatase catalyzes the substrate adamantane analog AMPPD, releases phosphate, and forms an unstable intermediate AMP-D, which then decomposes by itself. At the same time, photons are emitted. If there is a high concentration of phosphate in the detection reagent system, it will inhibit the catalytic effect of alkaline phosphatase on the substrate and affect the detection reaction.
Sodium dodecyl sulfate (SDS) in the surfactant 2 used in this lotion, because it is in powder form, the operator is easy to inhale when weighing, it has a stimulating effect on the mucous membrane and upper respiratory tract, and it is irritating to the eyes and skin It is harmful to the human body; SDS will destroy the protein structure at high concentrations, and improper use will cause the destruction of the antigen or antibody structure in the immune reaction, affecting the detection reaction; SDS solution is prone to precipitation at low temperature, which affects the washing effect of the lotion
[0006] CN107118865A discloses a cleaning solution, comprising phosphate buffer system, inorganic salt, surfactant A (Triton X-100 or Tween-20), surfactant B (sodium cholate) and preservative (Proclin300 or Proclin950), Also, this kind of cleaning solution uses a relatively high concentration of phosphate, which is not suitable for alkaline phosphatase chemiluminescence system
The surfactant sodium cholate used in the cleaning solution can dissolve the cell membrane and has a strong washing ability. Antigens or antibodies that have been specifically bound during the detection process are easily washed away, resulting in inaccurate detection results, and the molecular structure of sodium cholate Contains reactive nitrogen-hydrogen bonds, which are prone to non-specific reactions in immunoassays
In addition, the preservative Proclin300 or Proclin950 used in the cleaning solution is the same series of preservatives, which cannot achieve a good anticorrosion effect
[0007] CN108546602A discloses a cleaning solution for chemiluminescence immunoassay, including surfactant, non-protein protein protectant and buffering agent. The cleaning solution has complex components and high cost of use
The amide non-ionic surfactant used in the cleaning solution is easy to generate air bubbles during the washing and shaking process, which makes the detection of the liquid level of the instrument inaccurate and causes the value jump phenomenon
The pH of the cleaning solution is 5.32, which is slightly acidic and not suitable for the alkaline phosphatase system
In addition, the preservative Proclin300 used in the cleaning solution is a single preservative, which cannot achieve a good antiseptic effect
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
preparation example Construction
[0069] The preparation method provided by the invention has simple process and convenient operation, and the chemiluminescent cleaning liquid can be obtained by mixing raw materials, which is suitable for actual production and popularization and application.
[0070] In some preferred embodiments, metal ions, surfactants and preservatives are sequentially added to the buffer solution, and then an antifoaming agent is added, and then the chemiluminescence cleaning solution is obtained by constant volume with a solvent. By adding the components to the buffer, the components can be dispersed in a mild environment.
[0071] Preferably, the solvent comprises water.
[0072] More specifically, the final concentration of buffer in the chemiluminescence cleaning solution is 500 mM, the final concentration of sodium ion is 500 mM, the final concentration of CHAPS is 0.5w / v, the final concentration of TritonX-100 is 0.5v / v, and the final concentration of Proclin-300 is 0.6 %w / v, the fi...
Embodiment 1
[0084] This embodiment provides a kind of chemiluminescent cleaning solution, comprising:
[0085] 500mM Tris buffer, 0.5%w / v CHAPS, 0.5%w / v TritonX-100, 500mM NaCl, 0.6%w / v Proclin-300, 0.1%w / v BND and 0.1%w / v Propylparaben, 0.1% w / v silicone oil-type defoamer, pH 7.3, wherein the silicone oil-type defoamer is a silicone defoamer sold by Shanghai Sangong with a product number of A600465.
Embodiment 1-3-1
[0133] The difference between this embodiment and embodiment 1 is that the concentration of the Tris buffer is 100 mM, and the rest of the features are the same.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention provides a chemiluminescence cleaning solution and its preparation method and application, which relate to the technical field of in vitro diagnostic reagents for medical devices. The chemiluminescence cleaning solution provided by the invention mainly consists of a buffer, a surfactant, a metal ion and a preservative . Among them, the combined use of non-ionic surfactants and amphoteric surfactants makes the cleaning solution stable and accurate; the combination of different types of preservatives has a good anti-corrosion effect. The chemiluminescent cleaning liquid provided by the invention solves the problems of high price of imported cleaning liquid and high detection cost through the selection of specific components, has low cost, and reduces domestic dependence on imported cleaning liquid. At the same time, the chemiluminescent cleaning solution is suitable for the chemiluminescent immune reaction of the alkaline phosphatase system, and after project verification, the chemiluminescent cleaning solution can be applied to various alkaline phosphatase systems such as PCT, AMH, dsDNA, and CCP. Chemiluminescence detection project.
Description
technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents for medical devices, in particular to a chemiluminescence cleaning solution and its preparation method and application. Background technique [0002] The steps of the chemiluminescence immunoreaction include adding samples, incubating, washing, and interpreting. Among them, washing is an important step in determining the test results. The main purpose of washing is to remove unbound immunoreactive substances and free conjugates, terminate the continued binding of antigens and antibodies, and reduce the impact of non-specific interfering substances on the test results. The cleaning solution not only provides a suitable acid-base environment and electrolytes for the immune reaction, but more importantly, removes unbound immune reactants during the reaction and terminates the continued combination of antigens and antibodies. The surfactant in the cleaning solution can effectivel...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C11D1/94C11D3/04C11D3/16C11D3/34C11D3/20C11D3/60
CPCC11D1/72C11D1/88C11D1/94C11D3/042C11D3/162C11D3/2093C11D3/349
Inventor 张晓红张玉洁朱越谭曾敏霞
Owner ZHUHAI LIVZON DIAGNOSTICS