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Fish roe small molecule peptide mask liquid and preparation method of mask

A technology of small molecular peptides and facial masks, which can be used in medical preparations containing active ingredients, skin care preparations, pharmaceutical formulas, etc. It can solve the problems of poor dispersion uniformity of peptides, poor solubility of peptides, and hindrance to skin absorption. Achieve the effect of reducing hydrogen bond interaction, eliminating α-helix and β-sheet, and promoting continuous hydrolysis

Active Publication Date: 2020-09-08
张绍峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The β-turn also has the function of connecting other secondary structures (α-helix and β-sheet) in the protein molecule, making the peptide chain form a more stable complex non-repeating polypeptide region, making the solubility of the polypeptide poor and the dispersion uniformity of the polypeptide poor , which further hinders skin absorption and reduces its skin care effect

Method used

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  • Fish roe small molecule peptide mask liquid and preparation method of mask
  • Fish roe small molecule peptide mask liquid and preparation method of mask
  • Fish roe small molecule peptide mask liquid and preparation method of mask

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A kind of preparation method of caviar small molecular peptide facial mask, carries out as follows:

[0050] S01, caviar peptide extraction

[0051] (1) Mix the herring roe and purified water, high-speed shearing and crushing, the crushing temperature is 30°C, the rotation speed is 12000rpm, and the weight ratio of herring roe and purified water is 1:1;

[0052] (2) Soak the pulverized fish roe in a mixed solution with a volume ratio of methanol:ethylene glycol:cyclohexane=2:3:1, the ratio of solid to liquid is 1:35g / mL, at 25°C, stirring speed Degreasing at 400 rpm for 40 hours, then washing with purified water, and draining to form a degreased product for subsequent use;

[0053] (3) Add glycine-NaOH buffer solution with a pH of 8.8 to the degreased matter, the ratio of solid to liquid is 1:25g / mL, mix well; activate in a water bath at 40°C for 30min; add papain, the dosage is 1 / 2 of the degreased matter 2%, enzyme activity ≥ 2.5×10 4 U / g; enzymatic hydrolysis at 4...

Embodiment 2

[0093] A kind of preparation method of caviar small molecular peptide facial mask, carries out as follows:

[0094] S01, caviar peptide extraction

[0095] (1) Mix herring roe and purified water, high-speed shearing and crushing, the crushing temperature is 25°C, the rotation speed is 15000rpm, and the weight ratio of herring roe to purified water is 1:2;

[0096] (2) Soak the pulverized fish roe in a mixed solution with a volume ratio of methanol:ethylene glycol:cyclohexane=3:2:1, the ratio of solid to liquid is 1:15g / mL, at 30°C, stirring speed Degreasing at 200 rpm for 60 hours, then washing with purified water, and draining to form a degreased product for subsequent use;

[0097] (3) Add glycine-NaOH buffer solution with a pH of 8.4 to the degreased matter, the ratio of solid to liquid is 1:15g / mL, mix well; activate in a water bath at 30°C for 40min; add papain, the dosage is 0.5%, enzyme activity ≥ 2.5×10 4 U / g; enzymatic hydrolysis at 50°C for 55 minutes; after the r...

Embodiment 3

[0110] A preparation method of an anti-oxidation facial mask, carried out as follows:

[0111] S01, caviar peptide extraction

[0112] (1) Mix the herring roe and purified water, high-speed shearing and crushing, the crushing temperature is 40°C, the rotating speed is 10000rpm, and the weight ratio of herring roe to purified water is 1:0.5;

[0113](2) Soak the pulverized fish roe in a mixed solution with a volume ratio of methanol: ethylene glycol: cyclohexane = 1:5:1, the ratio of solid to liquid is 1:45g / mL, at 5°C, stirring speed Degreasing at 500 rpm for 15 hours, then washing with purified water, and draining to form a degreased product for subsequent use;

[0114] (3) Add glycine-NaOH buffer solution with a pH of 9.6 to the degreased matter, the ratio of solid to liquid is 1:30g / mL, and mix well; activate in a water bath at 55°C for 20min; 3%, enzyme activity ≥ 2.5×10 4 U / g; enzymatic hydrolysis at 50°C for 70 minutes; after the reaction was completed, the temperatur...

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Abstract

The invention discloses a preparation method of a fish roe small molecule peptide mask liquid. The method comprises small molecule peptide extraction and mask liquid preparation, and is characterizedin that: the small molecule peptide extraction comprises the following steps: pretreating fish roes, performing degreasing, hydrolyzing, impurity removal and settling to prepare fish roe polypeptide coarse powder, performing hydrolyzing by using a compound enzyme, and carrying out segmented separation and purification to extract small molecule peptides. The fish roe small molecule peptide mask prepared by the method is good in stability, low in peptide bond number of small molecule peptide and narrow in distribution ranging from 3 to 6. The small molecule peptides have high solubility in the mask, have excellent dispersion uniformity, are beneficial to uniform and effective absorption of the skin, have excellent oxidation resistance and permeability, and have the scavenging rates of 80.6%and 64.7% for DPPH free radicals and hydroxyl free radicals respectively.

Description

technical field [0001] The invention relates to the technical field of daily cosmetics, in particular to a method for preparing a fish roe small molecule peptide facial mask liquid and a facial mask. Background technique [0002] Skin oxidation is mainly due to a large number of free radicals produced by the skin during aerobic respiration. Excessive free radicals attack skin cells and rob the skin of nutrients, causing body aging, organ tissue mutations, skin aging and decreased immunity. The most important feature of oxidized skin is the yellowing of the complexion, the increase of spots and blemishes, just like the "yellow" of apples after oxidation, and the "rusting" of steel after oxidation. This damage occurs on the skin, causing relaxation and brittleness of collagen and elastic fibers, and slowing down of epidermal cell regeneration. Moreover, when the cell nucleus is damaged by free radicals, healthy cells cannot be divided, and the lipids between cells will also b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64A61K8/02A61Q19/08A61Q19/00
CPCA61K8/64A61K8/0212A61Q19/08A61Q19/00A61K2800/805
Inventor 黄河辛欣朱正宏
Owner 张绍峰
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