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Fluorescent microsphere combined detection device for myocardial marker and preparation method thereof

A myocardial marker and combined detection technology, which can be used in measurement devices, biological tests, material testing products, etc., can solve the problems of easy quenching of fluorescent antibodies, weak binding force, and insufficient amount of protein adsorbed by NC membranes, and improve the sensitivity. , reduce the dosage, and improve the effect of reasonable comprehensive judgment

Active Publication Date: 2020-09-08
JILIN PROVINCE GERUISITE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The present invention provides a fluorescent microsphere combined detection device for myocardial markers and a preparation method thereof, so as to solve the problems in the prior art that the fluorescent antibody is easily quenched, the amount of protein adsorbed by NC membrane is insufficient, and the binding force is not strong

Method used

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  • Fluorescent microsphere combined detection device for myocardial marker and preparation method thereof
  • Fluorescent microsphere combined detection device for myocardial marker and preparation method thereof
  • Fluorescent microsphere combined detection device for myocardial marker and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0046] The sample pad 1, the immunofluorescent antibody glass fiber membrane 2, the nitrocellulose membrane 3, and the absorbent pad 4 are respectively pasted on the plastic plate 5, and the two ends of the nitrocellulose membrane 3 are connected to the absorbent pad 4, the immunofluorescent antibody glass fiber membrane, respectively. 2 overlapping, the other end of the immunofluorescence antibody glass fiber membrane 2 is overlapped with the sample pad 1; the first detection line T1, the second detection line T2, the third detection line T3 and the first detection line T1 are set on the nitrocellulose membrane 3 Quality control line C; the solid phase of the first detection line T1 has highly specific MPO antibodies, the solid phase of the second detection line T2 has highly specific cTnI or cTnT antibodies, and the third detection line There is a highly specific H-FABP antibody on the solid phase of T3, and there is a highly specific NT-proBNP antibody on the solid phase of ...

Embodiment 2

[0066] Preparation of polyethylene glycol glycerin treatment solution: mixed with polyethylene glycol glycerin and polylysine (SIGMA, 150KD), wherein the concentration of polyethylene glycol glycerin is 0.5%, and polylysine The concentration is 0.5%, filtered through a 0.22μm filter membrane, and set aside.

[0067] All the other are with embodiment 1.

Embodiment 3

[0069] Preparation of polyethylene glycol glycerin treatment solution: mixed with polyethylene glycol glycerin, polylysine (SIGMA, 150KD) and PEG2000, wherein the concentration of polyethylene glycol glycerin is 0.5%, poly The concentration of lysine is 0.5%, and the concentration of PEG20000 is 0.1%, and it is filtered through a 0.22 μm filter membrane for later use.

[0070] All the other are with embodiment 1.

[0071] Further illustrate the effect of the present invention by experiment below.

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Abstract

The invention relates to a fluorescent microsphere combined detection device for a myocardial marker and a preparation method thereof, and belongs to the field of medical detection equipment. The device is formed by adhering a nitrocellulose membrane with a solid phase containing high-specificity MPO, cTnI or cTnT, H-FABP and NT-proBNP antibody and goat-anti-mouse IgG polyclonal antibody, glass fibers adsorbed with fluorescent microsphere labeled MPO, cTnI or cTnT, H-FABP and NT-proBNP antibodies, a sample pad, absorbent paper and other auxiliary materials through adhesion. The preparation method comprises the following steps: pretreating a nitrocellulose membrane by adopting a polyethylene glycol glycerol treatment solution; firstly combining MPO, cTnI or cTnT, H-FABP and NT-proBNP antibodies with oleic acid modified zinc sulfide nanoparticles and then adsorbing the combined antibodies onto a nitrocellulose membrane; and preparing a proper buffer solution and a sample pad treatment solution; on the basis of ensuring complete release of the immunofluorescence microspheres, the reaction sensitivity is effectively improved, the dosage of the immunofluorescence microspheres can be reduced under the same threshold value, four markers of MPO, cTnI or cTnT, H-FABP and NT-proBNP in a specimen can be detected at the same time, and the invention is high in sensitivity, specificity and practicability.

Description

technical field [0001] The invention relates to the field of medical detection equipment, in particular to a fluorescent microsphere combined detection device for myocardial markers and a preparation method thereof, which can quantitatively detect human myeloperoxidase (MPO) and cardiac calcium in whole blood, serum and plasma samples Protein I or T (cTnI or cTnT), heart-type fatty acid binding protein (H-FABP) and N-terminal pro-brain natriuretic peptide (NT-proBNP), can realize sensitive, specific and rapid detection of myocardial markers. Background technique [0002] Acute coronary syndrome (acute coronary syndrome, ACS) refers to the pathological phenomenon of atherosclerotic plaque rupture, platelet aggregation, thrombus formation leading to coronary stenosis, obstruction, myocardial ischemia and infarction, including acute myocardial infarction (acute myocardial infarction) myocardial infarction, AMI) and unstable angina (unstable angina, UA). AMI is further divided ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/68G01N33/573G01N33/58G01N33/558G01N33/543
CPCG01N33/54346G01N33/558G01N33/573G01N33/582G01N33/6887G01N33/74G01N2333/47G01N2333/4712G01N2333/58G01N2333/908G01N2800/324
Inventor 杨小军李欣
Owner JILIN PROVINCE GERUISITE BIOTECHNOLOGY CO LTD