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Low-cost bamboo leaf flavonoid powder and preparation method thereof

A low-cost technology of bamboo leaf flavonoids is applied in the field of separation and purification, and can solve the problems of low product yield, complicated separation and purification treatment, and difficulty in scale.

Pending Publication Date: 2020-09-11
GUIZHOU UNIV OF ENG SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many inventions and creations using bamboo leaves to extract flavonoids at home and abroad, but there are some problems in these technologies. One is the high cost, mainly using organic solvents to extract, separate and purify, and there are large solvent consumption and loss, easy to produce environmental pollution, and the extract contains impurities. There are many problems, such as complex separation and purification treatment; second, large-scale difficulty and slow pace, there are problems such as low collection of total flavonoids in bamboo leaves, low product yield and complicated purification technology

Method used

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  • Low-cost bamboo leaf flavonoid powder and preparation method thereof
  • Low-cost bamboo leaf flavonoid powder and preparation method thereof
  • Low-cost bamboo leaf flavonoid powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Put 50kg of fresh bamboo leaves of Phyllostachys pubescens into a 400L multi-functional extraction tank, under the condition of 80°C, solid-liquid ratio 1:8g / mL, 85% methanol solution, 3h / time, leaching 3 times, combine the extracts and reduce Press and concentrate to a thin liquid paste, add commercially available wheat starch into the liquid paste to make a non-sticky, hand-tearable soft dough, extrude into strips through a particle vibrating sieve machine (aperture 0.5cm), freeze-dried, Pulverize into 200 order powder, obtain the mixed sample product 1.22kg.

Embodiment 2

[0055] Take 1.0 kg of the above mixed sample product, put it on the column (20×80cm) according to the silica gel dry method, carry out column chromatography separation, and elute with petroleum ether: ethyl acetate = 1:1 until the color of the eluent is light , change to anhydrous methanol for elution, collect the eluate, set the HPLC to detect at 254nm and 360nm wavelengths, and show strong absorption peaks under dual-wavelength conditions, indicating that flavonoids are contained in the methanol eluent. HPLC detection is as follows: figure 2 shown.

Embodiment 3

[0057] Take the collected solution above, add 200-300 mesh silica gel, concentrate and mix the sample under reduced pressure, until the mixed sample is dry and free of methanol, use petroleum ether as solvent, and apply column chromatography separation method by wet method (column ), the blank solvent petroleum ether was applied to the column, and after the residual pigment impurities were eluted with petroleum ether:ethyl acetate=1:1, the gradient elution of ethyl acetate to anhydrous methanol=1:1 was used instead, and the eluent was collected , HPLC detection (at 254nm and 355nm wavelength), the absorption peaks all have double absorption peaks, showing that it is a flavonoid compound composition, and the HPLC detection spectrum is as follows image 3 shown.

[0058] According to the technical scheme of the present invention, making full use of abundant bamboo natural resources and considering the characteristics of the physical and chemical properties of bamboo leaf compon...

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Abstract

The invention discloses a preparation method of low-cost bamboo leaf flavonoid powder. The preparation method comprises the steps of obtaining diluted flow paste by leaching with resolvent and concentrating bamboo leaf raw materials, adding starch, performing stirring and kneading to obtain block masses, performing drying, and performing grinding to obtain starch mixed powder; performing dry column-packing on the starch mixed powder and blank starch, firstly, performing gradient elution with a petroleum ether-ethyl acetate flow phase, and performing eluting with an alcohol flow phase; collecting an eluent of the alcohol flow phase, adding a silica gel mixing sample, and performing drying to obtain silica gel mixed substances; performing wet column-packing on the silica gel mixing sample and blank silica gel, firstly, performing gradient elution with the petroleum ether-ethyl acetate flow phase, and then performing gradient elution with an ethyl acetate-alcohol flow phase; and then concentrating and drying the eluent containing flavones, of the ethyl acetate-alcohol flow phase, so as to obtain the bamboo leaf total flavonoid powder. The invention further discloses the bamboo leaf flavonoid powder prepared by the method.

Description

technical field [0001] The invention relates to the field of separation and purification, in particular to a low-cost bamboo leaf flavonoid powder and a preparation method thereof. Background technique [0002] There are many active ingredients in bamboo leaves, but bamboo leaf flavonoids are one of the main active ingredients. The content and biological activity of total flavonoids in bamboo leaves are comparable to those of ginkgo biloba. Bamboo leaf flavonoids are a kind of mixed total flavonoids. According to the current structural identification and analysis, bamboo leaf flavonoids contain more than 30 kinds of flavonoids, including flavonoid aglycone and flavonoid glycosides, among which isoorientin, orientin, and peony in carbon glycoside flavonoids Vitexin and isovitexin are the main characteristic flavonoids. Flavonoids in bamboo leaves have obvious effects of lowering blood fat and cholesterol. Pharmacological studies have shown that bamboo leaf flavonoids have t...

Claims

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Application Information

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IPC IPC(8): A61K36/899A61K127/00
CPCA61K36/899A61K2236/33A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 许子竞陶文亮杨玉琼
Owner GUIZHOU UNIV OF ENG SCI