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Buddleja officinalis extract, and preparation method and application thereof

A technology of dense Mongolian flowers and extracts, applied in the field of medicine, can solve problems such as unresearched, complex chemical composition, and inability to know

Active Publication Date: 2020-09-15
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The prescription is composed of six medicinal materials including Astragalus, Ligustrum lucidum, Motherwort, Coptis chinensis, Cinnamon and Mimenghua. Pharmacological activity of HUVEC proliferation, which pharmacological components can act on VEGF-VEGFR signaling pathway and have pharmacological activity of inhibiting HUVEC proliferation under hypoxia cannot be known from its disclosure

Method used

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  • Buddleja officinalis extract, and preparation method and application thereof
  • Buddleja officinalis extract, and preparation method and application thereof
  • Buddleja officinalis extract, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0094] Preparation Example 1: Extraction of active ingredients in Budisma japonica by water extraction and alcohol extraction

[0095] Weigh 100g of Mimenghua traditional Chinese medicine decoction pieces, add 1000ml of 95% ethanol, infiltrate for 0.5h, heat and reflux at 80°C for 1h, filter and collect the filtrate; add 1000ml of 95% ethanol to the filter residue, heat and reflux for 1h at 80°C, filter and combine twice The filtrate was concentrated under reduced pressure using a rotary evaporator to obtain a 95% extract concentrate of Budhimonica japonica, which was freeze-dried (19.6 g), and coded as MMH-95.

[0096] Add 1000ml of 50% ethanol to the filter residue after extraction with 95% ethanol, heat and reflux at 100°C for 1 hour, and collect the filtrate by filtration; Concentrate under pressure to obtain 50% Budisma japonica extract extract, and use a freeze dryer to freeze-dry to obtain a solid sample, numbered MMH-95-50 (11.3g).

[0097] Weigh 100g of Mimenghua tra...

preparation Embodiment 2

[0100] Preparation Example 2: Water Extraction-Solvent Extraction Separation of Active Components in Budisma Buddhisattva

[0101] Weigh 100g of Mimenghua traditional Chinese medicine decoction pieces, add 1000ml of distilled water, infiltrate for 0.5h, heat and reflux at 100°C for 1h, filter and collect the filtrate; Concentrate under reduced pressure with an evaporator to obtain 200ml of water-extracting concentrated solution of Budisma japonica, add 200ml of ethyl acetate solvent for extraction, let it stand until the layers are separated, collect the upper layer of ethyl acetate solution, perform the same extraction for 5 times, combine the ethyl acetate extract, and reduce Press and dry to obtain ethyl acetate extraction sample, number MMH-H 2 O-EA; add 200ml of n-butanol solvent to extract the remaining water layer, let it stand until the layers are separated, collect the n-butanol solution in the upper layer, perform the same extraction for 5 times, combine the n-butano...

preparation Embodiment 3

[0102] Preparation Example 3: Purification and Identification of Compounds I~V

[0103] Take by weighing the MMH-C-H obtained in Preparation Example 2 2 O sample 5g is added appropriate amount of distilled water heating ultrasonic dissolving, adopt macroporous resin (D101) to carry out column chromatography separation (500 * 60mm), first adopt distilled water 1000ml to wash, adopt 20% ethanol 1000ml to wash subsequently and collect eluate, this washing The deliquored liquid contains main characteristic peaks through HPLC analysis, and the purity is above 70%. It is concentrated and combined to obtain a sample eluted with 20% ethanol. The sample was purified again with ODS chromatography packing, eluted with distilled water and 10% methanol successively, concentrated and combined with 10% methanol eluate, and analyzed by HPLC, the purity was 94.14%. It was concentrated and dried under reduced pressure to obtain 86.2 mg of brown yellow solid powder, coded as Compound Ⅰ, which w...

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Abstract

The invention discloses a buddleja officinalis extract, a preparation method thereof, and an application of the buddleja officinalis extract to preparation of a medicine for treating or preventing retinal degenerative changes. The buddleja officinalis extract is obtained by extracting buddleja officinalis with an aqueous solution, and the total weight of five compounds including apigenin-7,4'-di-O-beta-D-glucosiduronic acid, echinacoside, verbascoside, isoverbascoside and apigenin-7-O-rutinoside accounts for 10% or above of the total weight of the extract. Experiments prove that the extract has good inhibitory activity on VEGFR-1 and VEGFR-2, can significantly inhibit occurrence of retinal photoreceptor cell degenerative changes, and can be used for treating or preventing retinal degenerative changes.

Description

technical field [0001] The present invention belongs to the technical field of medicine, in particular, the present invention relates to an extract of Budhimonica japonica, its preparation method, and its use in the preparation of medicines for treating or preventing retinal degeneration, and The pharmaceutical composition of the substance. Background technique [0002] Degenerative retinal disease is a kind of retinal disease with retinal photoreceptor cell death as the core pathological change, which can cause severe visual impairment or even blindness. It is a common severe blinding eye disease in clinical practice, mainly including age-related macular degeneration (age-related macular degeneration, AMD), diabetic retinopathy (diabetic retinopathy, DR) and retinitis pigmentosa (retinitis pigmentosa, RP) and other diseases. Among them, AMD is the primary cause of low vision and blindness in the elderly in developed countries. There are about 30 million age-related macular...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H15/18C07H1/08A61K31/7048A61K31/7032A61P27/02
CPCA61P27/02C07H1/08C07H15/18C07H17/07
Inventor 张勇陈瑜李波杜霄烨徐静徐志建蔡婷婷张腾朱维良
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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