Nano-carrier material and preparation method and application thereof to preparation of antitumor drugs

An anti-tumor drug and nano-carrier technology is applied in the field of biomedical engineering materials to achieve the effects of good biocompatibility, improved biocompatibility and good biocompatibility

Active Publication Date: 2020-09-22
BEOGENE BIOTECH GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biggest challenge in the co-delivery of chemotherapeutics and genes for tumor combination therapy is the synthesis of safe and efficient carrier materials.

Method used

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  • Nano-carrier material and preparation method and application thereof to preparation of antitumor drugs
  • Nano-carrier material and preparation method and application thereof to preparation of antitumor drugs
  • Nano-carrier material and preparation method and application thereof to preparation of antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Preparation and Characterization of AuNR

[0048] Seed Synthesis:

[0049] Take 10mL of 0.1M CTAB solution into a 15mL glass bottle, then add 250μL of 0.01M HAuCl 4 After stirring the solution at a constant speed for about 1 min, add 600 μL of NaBH with a concentration of 0.01M 4 , and stirred for 2 minutes, the system was a brown solution at this time, which was placed at 25° C. for 2 hours to obtain a seed solution.

[0050] Seed Growth:

[0051] Take 40mL of 0.1M CTAB solution into a 100mL beaker, add 2mL of 0.01M HAuCl 4 Solution, the system is orange at this time, then add 400 μL of AgNO with a concentration of 0.01M 3 Solution, after stirring for 5 minutes, 320 μL of AA with a concentration of 0.1M was added, and the system changed from orange to colorless, and then 800 μL of HCl and 96 μL of the above-mentioned synthetic seed solution were added, and left at room temperature overnight to obtain a wine-red gold nanorod solution. Centrifuge and take...

Embodiment 2

[0053] Embodiment 2: the preparation of DSPAMAM

[0054] 300 mg of 3,3'-dithiodipropionic acid was dissolved in 10 mL of anhydrous dimethyl sulfoxide, activated by adding EDC (1.15 g) and NHS (0.69 g), and stirred at room temperature for 4 hours. Then the solution was dropped into the DMSO solution containing 250 mg PAMAM, and the reaction was stirred at room temperature for 12 hours. Finally, the reactant mixture was dialyzed in pure water for 3 days using a dialysis bag (molecular weight: 3500) to remove excess reactant, and then dried to obtain DSPAMAM.

Embodiment 3

[0055] Example 3: AuNR@SiO 2 Preparation and characterization of @DSPAMAM nanocarrier materials

[0056] Dilute 4 mL of the rod-shaped gold nanoparticle AuNR synthesized in Example 1 with 10 mL of water, pour it into a glass bottle, and sonicate for 5 min, then add 200 μL of 0.1 mol / L aqueous sodium hydroxide solution and sonicate for 5 min. Finally, add 200 μLTEOS, adjust the rotation speed to not more than 500 rpm, and stir for two days. After the reaction, centrifuge at 9000rpm for 30min to obtain AuNR@SiO 2 , 2mL AuNR@SiO 2 Resuspended in 10 mL of DSPAMAM solution with a concentration of 10 mg / mL, stirred ultrasonically for 2 hours, and centrifuged to remove the supernatant to obtain AuNR@SiO 2 @DSPAMAM.

[0057] The prepared AuNR@SiO by transmission electron microscopy 2 @DSPAMAM for characterization like figure 2 As shown, the outer layer of AuNR is attached with a silica layer, which is uniformly dispersed.

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Abstract

The invention discloses a nano-carrier material. The nano-carrier material comprises gold nano particles, a SiO2 layer wrapping the gold nano particles, and a DSPAMAM layer wrapping the SiO2 layer, and DSPAMAM is disulfide cross-linked PAMAM. The nano-carrier material is easy to prepare, preparation raw materials are easy to obtain, quantitative production is easy to realize, and the advantages ofAuNR, SiO2 and the DSPAMAM are integrated; the biocompatibility is good, and a CT imaging function and GSH responsiveness are realized; the drug and gene loading function is further realized; an anti-cancer drug, namely CUR and a functional gene (miRNA) can be efficiently co-delivered in treatment of prostatic carlcer; and RGD is used as a targeting polypeptide, the RGD polypeptide can promote enrichment of nano-materials in tumor cells, drug controlled release can be achieved, side effects of the drugs can be reduced, and efficient diagnosis and combined treatment of human prostate cancer can be further realized.

Description

technical field [0001] The invention belongs to the technical field of biomedical engineering materials, and in particular relates to a nanometer carrier material and its preparation method and its application in the preparation of antitumor drugs. Background technique [0002] Cancer is a disease caused by the loss of normal regulation and excessive proliferation of body cells, also known as malignant tumors. Cancer cells are characterized by abnormal division, uncontrolled growth, and the ability to cause disease in adjacent tissues and organs. Moreover, cancer cells can also spread out from the tumor, enter the blood or lymphatic system and transfer to other parts of the body to form new tumors, eventually causing damage to the patient's internal organs and even death. So far, the mortality rate of cancer is still as high as about 50%, so seeking effective cancer treatment methods has become an urgent problem to be solved today. [0003] At present, the clinical treatme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K31/12A61K31/7088A61K47/62A61K47/69A61K49/04A61P13/08A61P35/00B22F1/00B22F1/02B22F9/24B82Y5/00B82Y40/00C08G73/02
CPCA61K41/0052A61K47/62A61K47/6935A61K31/12A61K31/7088A61K49/04C08G73/028A61P35/00A61P13/08B82Y5/00B22F9/24B82Y40/00B22F1/07B22F1/054B22F1/16A61K2300/00
Inventor 文荣冯龙宝蓝咏刘玉
Owner BEOGENE BIOTECH GUANGZHOU
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