Highly compatible virus preserving fluid for paramagnetic particle method virus nucleic acid extracting kit

Abstract

The invention discloses a highly compatible virus preserving fluid for a paramagnetic particle method virus nucleic acid extracting kit. The highly compatible virus preserving fluid comprises the following components: guanidine hydrochloride, tris (hydroxymethyl) aminomethane hydrochloride, ethylenediamine tetraacetic acid, isopropanol or ethanol. The virus preserving fluid disclosed by the invention belongs to an inactivated type, has the effects of preserving and cracking a virus sample, and can be compatible with various common paramagnetic particle method virus nucleic acid extracting kitsin the market; during nucleic acid extraction, the sample and a lysate can be mixed according to any proportion between the proportion suggested by the kit and 100%; and in other words, less or evenno lysate can be added, so that the use amount of the sample is increased, and more virus nucleic acids and more accurate detection results are obtained. The virus preserving fluid has the advantagesof simple components, easily available raw material and relatively low cost, can preserve a sample at room temperature for a long time, does not need high-temperature inactivation, is very safe to operators and the environment, reduces the possibility of RNA degradation, can obtain relatively more nucleic acids, and can lower the omission ratio.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a virus preservation solution highly compatible with a magnetic bead method virus nucleic acid extraction kit. Background technique [0002] In modern medical tests, it is often necessary to extract nucleic acids (DNA, RNA) from suspected samples to detect the presence of pathogenic viruses. Due to the limitation of time or distance, the samples to be tested generally need to be stored for a period of time or sent to a specific location for testing. [0003] At present, there are virus preservation solutions on the market that can meet the above requirements, such as Hank's solution and PBS solution, but there are also some defects: due to the low virus content in many samples, after dilution of about 3mL of preservation solution, only a maximum of 200μL of sample-containing solution is taken. Nucleic acid extraction is carried out after the preservation solution is lysed by...

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Problems solved by technology

[0003] At present, there are virus preservation solutions on the market that can meet the above requirements, such as Hank's solution and PBS solution, but there are also some defects: due to the small amount of virus in many samples, after being diluted with about 3mL preservation solution, only a maximum of 200 μL of the sample containing the sample can be taken. Nucleic acid is extracted after the preservation solution is lysed by the lysate, so the amount of nucleic acid obtained is very low, which may lead to negative results in subsequent tests (such as fluorescent quantitative PCR) and affect the judgment of the disease

The amount of sample extracted by the kit is relatively small, and the standard sample addition is 200 μL. The reason is that Hank's solution or PBS is used for conventional virus preservation. Increasing the sample size will dilute the salt concentration of the lysate, resulting in a decrease in nucleic acid yield.

At the same time, when the sample volume is less than 200 μL, it is required to make up the volume with PBS, which will also lead to a low nucleic acid yield

Application number 202010128653.3 discloses a sample preservation solution based on guanidine thiocyanate, but this type of sample preservation solution containing guanidine thiocyanate will lead to the inactivation of proteinase K in the nucleic acid extraction reagent and the formation of magnetic beads, which will eventually lead to Nucleic acid yield decreased

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