Dense double-layer biological membrane, preparation method and application
A technology of biofilm and biomedical materials, applied in medical science, prosthesis, etc., can solve the problems of increased operational complexity, no interaction force, increased cost, etc., to improve tissue repair effect, prevent ingrowth and proliferation, The effect of increasing tightness
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Embodiment 1
[0030] A method for preparing a dense double-layer biofilm, comprising the following steps:
[0031] S1, chitosan is made into a solution of 8wt% as a dense layer solution; then chitosan is made into a solution of 2wt% as a loose layer solution; the pH of the dense layer solution is 3.4, and the loose layer The pH of the solution is 4.5; the dense layer solution and the loose layer solution are degassed by centrifuging the dense layer solution and the loose layer solution at a temperature of 4°C and a speed of 3200 rpm for 10 minutes, respectively;
[0032] S2, pour the dense layer solution into a custom-made utensil, shake it evenly, and air-dry it at a temperature of 10°C and a humidity of 50% until the concentration of the dense layer solution is 60wt%, and then place it at -30°C for 2 hours to form a dense layer ;
[0033] S3, pour the loose layer solution onto the dense layer frozen in step S3, oscillate evenly, and thaw at 2°C; after the dense layer melts, put it in a r...
Embodiment 2
[0039] A method for preparing a dense double-layer biofilm, comprising the following steps:
[0040] S1, make hyaluronic acid into a 10wt% solution as the compact layer solution; then make hyaluronic acid into a 2.5wt% solution as the loose layer solution; set the pH of the dense layer solution to 3.8, and the loose layer solution The pH of the layer solution is 5.0; the dense layer solution and the loose layer solution are degassed by centrifuging the dense layer solution and the loose layer solution at a temperature of 3 ° C and a speed of 3200 rpm for 8 minutes;
[0041] S2, pour the dense layer solution into a custom-made utensil, shake it evenly, and air-dry it at a temperature of 8°C and a humidity of 40% until the concentration of the dense layer solution is 50wt%, and then put it in -20°C for 1.5 hours to form a dense layer ;
[0042] S3, pour the loose layer solution onto the dense layer frozen in step S3, oscillate evenly, and thaw at 4°C; after the dense layer melt...
Embodiment 3
[0048] A method for preparing a dense double-layer biofilm, comprising the following steps:
[0049] S1, sodium alginate is made into a solution of 8wt% as a dense layer solution; then sodium alginate is made into a solution of 2wt% as a loose layer solution; the pH of the dense layer solution is 3.2, and the loose layer The pH of the solution is 4.0; the dense layer solution and the loose layer solution are degassed by centrifuging the dense layer solution and the loose layer solution at a temperature of 4 ° C and a speed of 3200 rpm for 10 minutes;
[0050] S2, pour the dense layer solution into a custom-made utensil, shake it flat, air-dry it at a temperature of 15°C and a humidity of 70% until the concentration of the dense layer solution is 60wt%, and then place it in -30°C for 1 hour to form a dense layer ;
[0051] S3, pour the loose layer solution onto the dense layer frozen in step S3, oscillate evenly, and thaw at 2°C; after the dense layer melts, put it in a refriger...
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