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Lactobacillus fermentum with effect of relieving osteoporosis and application thereof

A technology of Lactobacillus fermentum and osteoporosis, applied in the field of microorganisms, can solve the problems of poor universality, patient dependence and tolerance, embolism, etc., and achieve the effect of strong universality and wide application

Active Publication Date: 2020-09-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these drugs have their own metabolic characteristics and applicable populations, and their general applicability is not strong (for example, zoledronic acid is only applicable to postmenopausal osteoporosis populations), and some drugs may cause some special adverse reactions after long-term use ( For example, the selective estrogen receptor modulator raloxifene can cause venous thromboembolism and stroke), some drugs can cause dependence and tolerance in patients after long-term use, and withdrawal symptoms will occur once the drug is stopped Various drug withdrawal reactions

Method used

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  • Lactobacillus fermentum with effect of relieving osteoporosis and application thereof
  • Lactobacillus fermentum with effect of relieving osteoporosis and application thereof
  • Lactobacillus fermentum with effect of relieving osteoporosis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Embodiment 1: the acquisition of Lactobacillus fermentum

[0079] Specific steps are as follows:

[0080] Take fresh feces from healthy people in Enshi, Hubei as samples, add 0.5mL sample to 5mL MRS liquid medium, and incubate at 37°C for 18-24h to obtain enriched samples; draw 0.5mL enriched The pooled sample was added to 4.5mL sterile saline to obtain 10 -1 Diluent, then draw 0.5mL 10 -1 Diluted in 4.5mL of normal saline to obtain 10 -2 Diluent, according to this operation, successively get 10 -3 , 10 -4 , 10 -5 , 10 -6 Dilution; pipette 100 μL of gradient dilution and spread on MRS solid medium, 10 -4 , 10 -5 , 10 -6 1 plate for each gradient, cultivated at 37°C for 48 hours to obtain colonies; select colonies with typical characteristics of Lactobacillus fermentum on the MRS solid medium according to the shape, size, edge, and transparency of the colonies, and use an inoculation loop to pick the colonies on the MRS solid culture Streak on the base, culture...

Embodiment 2

[0086] Example 2: Effect of Lactobacillus fermentum on Uterine Coefficient of Ovariectomized Rats

[0087] Specific steps are as follows:

[0088] Take 30 SPF grade female SD rats with a body weight of 250±20g, and divide them into 6 groups randomly, 5 rats in each group, and the 6 groups are: sham operation group, model control group, and positive control of intragastric administration of sodium alendronate solution group, CCFM1126 group fed with Lactobacillus fermentum CCFM1126 suspension, FZJTZ20 group fed with Lactobacillus fermentum FZJTZ20 suspension and L10 group fed with L10 suspension.

[0089] The experiment lasted for 9 weeks: the first week was the adaptation period for the rats. During the adaptation period, the rats were fed in an environment with a temperature of 22±2°C, a humidity of 40-70%, and a 12-hour cycle of day and night, free to eat and drink, and the feed used was purchased from Suzhou Shuangshi Rat breeding compound feed from Experimental Animal Feed...

Embodiment 3

[0094] Example 3: Effects of Lactobacillus fermentum on bone mineral density and cortical volume in ovariectomized rats

[0095] Specific steps are as follows:

[0096] Take 30 SPF grade female SD rats with a body weight of 250±20g, and divide them into 6 groups randomly, 5 rats in each group, and the 6 groups are respectively: sham operation group, model control group, positive control of intragastric administration of sodium alendronate solution group, CCFM1126 group fed with Lactobacillus fermentum CCFM1126 suspension, FZJTZ20 group fed with Lactobacillus fermentum FZJTZ20 suspension and L10 group fed with L10 suspension.

[0097] The experiment lasted for 9 weeks: the first week was the adaptation period for the rats. During the adaptation period, the rats were fed in an environment with a temperature of 22±2°C, a humidity of 40-70%, and a 12-hour cycle of day and night, free to eat and drink. The feed used was purchased from Suzhou Shuangshi Rat breeding compound feed fr...

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Abstract

The invention discloses a lactobacillus fermentum with an effect of relieving osteoporosis and an application thereof, belongs to the technical field of microorganisms and the technical field of medicine and provides a lactobacillus fermentum CCFM1126. The lactobacillus fermentum CCFM1126 can prevent and / or treat osteoporosis, and has the specific effects of improving uterine coefficients of ovariectomized rats, significantly increasing the bone density of the ovariectomized rats, significantly increasing the cortical volume of the ovariectomized rats, significantly reducing the content of tartrate-resistant acid phosphatase in serum of the ovariectomized rats, significantly reducing the content of a type I collagen cross-linked C-terminal telopeptide in the serum of the ovariectomized rats, significantly reducing the content of a tumor necrosis factor-alpha in the serum of the ovariectomized rats, reducing the content of interleukin-1beta in the serum of the ovariectomized rats and significantly improving the content of estradiol in the serum of the ovariectomized rats.

Description

technical field [0001] The invention relates to a strain of lactobacillus fermentum with the effect of relieving osteoporosis and its application, belonging to the technical field of microorganisms and the technical field of medicine. Background technique [0002] Osteoporosis is a systemic skeletal disease characterized by decreased bone mass and degeneration of bone microstructure, resulting in increased bone fragility. Some clinical symptoms are commonly used to diagnose osteoporosis, including: low back, limb pain, spinal deformity, fracture, etc. [0003] According to the incidence rate and demographic data, osteoporosis is one of the diseases with the largest morbidity, mortality and health care costs in the world. In my country alone, more than 70 million osteoporosis patients are suffering from the disease, among which postmenopausal osteoporosis patients account for about 80%. [0004] Compared with the normal population, the mortality rate of osteoporotic patient...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P19/10C12R1/225
CPCC12N1/20A23L33/135A61K35/747A61P19/10A23V2002/00C12R2001/225C12N1/205A23V2400/143A23V2200/306Y02A50/30
Inventor 翟齐啸陈卫彭江于雷雷田丰伟赵建新张灏王刚崔树茂陆文伟
Owner JIANGNAN UNIV
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