Method for extracting hesperidin from fructus aurantii immaturus

A technology of hesperidin and citrus aurantium, which is applied in the field of separation and extraction of natural products, can solve the problems affecting the purity of diosmin and drug efficacy in semi-synthetic products, and achieve the effect of consistent process, high content and low production cost

Active Publication Date: 2020-10-02
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] It can be seen that if there is isonaringin in the finished hesperidin product, it will greatly affect the purity of diosmin in the semi-synthetic product and the efficacy of the drug

Method used

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  • Method for extracting hesperidin from fructus aurantii immaturus
  • Method for extracting hesperidin from fructus aurantii immaturus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) Degreasing: take 100kg of Citrus citrus raw material, crush it to a particle size of 1-1.5mm, put it into an extraction tank, add petroleum ether, heat and reflux for extraction twice (the first time is 900L, and the extraction time is 2 hours; the second time is 600L , extraction time 2 hours), filtered to obtain extraction slag.

[0051] (2) Enzymolysis: Distill the residual organic solvent in the extraction residue in step (1) with steam, add 900L pure water, adjust the pH value to 5.0, and add 0.2kg cellulase and 0.2kg protopectinase , mix well, and stir at room temperature for 6 hours for enzymatic hydrolysis.

[0052] (3) Impurity removal and centrifugation: heat the material after enzymatic hydrolysis in step (2) to 85° C., stir and extract for 3 hours, cool to room temperature, and centrifugally filter to obtain centrifuge residue.

[0053] (4) Extraction and separation of hesperidin: step (3) centrifugal slag is put into the percolator, and 600L lye is add...

Embodiment 2

[0056] (1) Degreasing: take 100kg of Fructus Citrus citrus raw material, crush it to a particle size of 1-1.5mm, put it into an extraction tank, add 6# solvent oil, heat and reflux for extraction twice (1000L for the first time, 2 hours for extraction time; time 600L, extraction time 1.5 hours), filtered to obtain extraction residue for later use.

[0057] (2) Enzymolysis: Distill the residual organic solvent in the extraction residue in step (1) with steam, add 800L of pure water, adjust the pH value to 5.5, and add 0.1kg of cellulase and 0.2kg of protopectinase , mix well, and stir at room temperature for 5 hours for enzymatic hydrolysis.

[0058] (3) Impurity removal and centrifugation: heat the material after enzymatic hydrolysis in step (2) to 90° C., stir and extract for 3 hours, cool to room temperature, and centrifuge to obtain a centrifuge residue.

[0059] (4) Extraction and separation of hesperidin: step (3) centrifugal slag is put into the percolator, and 500L lye...

Embodiment 3

[0062] (1) Degreasing: take 100kg of Citrus citrus raw material, crush it to a particle size of 1-1.5mm, put it into an extraction tank, add cyclohexane, heat and reflux for extraction twice (the first time is 800L, and the extraction time is 3 hours; the second time 700L, extraction time 2 hours), filtered to obtain extraction residue.

[0063] (2) Enzymolysis: distill the residual organic solvent from the extraction residue in step (1) with steam, add 1000L of pure water, adjust the pH value to 6.0, and add 0.3kg of cellulase and 0.2kg of protopectinase , mix well, and stir at room temperature for 4.5 hours for enzymatic hydrolysis.

[0064] (3) Impurity removal and centrifugation: heat the material after enzymatic hydrolysis in step (2) to 95°C, stir and extract for 2 hours, cool to room temperature, and centrifuge to obtain centrifuge residue.

[0065] (4) Extraction and separation of hesperidin: step (3) centrifugal slag is put into the percolator, and 600L lye is added ...

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Abstract

The invention provides a method for extracting hesperidin from fructus aurantii immaturus. The method comprises the following steps of degreasing, enzymolysis, impurity removal, percolation, diatomiteadsorption and two-step pH regulation, thereby finally obtaining the fructus aurantii immaturus hesperidin. The method for extracting high-quality hesperidin from fructus aurantii immaturus providedby the invention is coherent and simple in process, high in operability, low in acid and alkali consumption, low in sewage discharge, low in production cost, free of toxic and harmful chemical solvents, safe, environmentally friendly, high in hesperidin content, high in yield and suitable for industrial production. In addition, the hesperidin product produced by the method is high in content and does not contain isonaringin. The hesperidin prepared by the method is used for synthesizing diosmin, and the diosmin product prepared from the hesperidin as a raw material is high in purity and good in drug effect due to no interference of isonaringin.

Description

technical field [0001] The invention relates to the separation and extraction of natural products, in particular to a method for extracting hesperidin from Citrus aurantium. Background technique [0002] Citrus aurantium, Rutaceae plant sour orange Citrus aurantium L. and its cultivars or sweet orange Citrussinensis Osbeck dried young fruit. Sour oranges are mainly produced in Jiangxi, Sichuan, Hubei, Hunan, Zhejiang, Jiangsu, Fujian, Guizhou and other places; sweet oranges are cultivated in Jiangsu, Zhejiang, Jiangxi, Sichuan, Guizhou, Fujian, Taiwan, Guangdong, Guangxi, Yunnan and other places . [0003] In the field of traditional Chinese medicine, Citrus aurantium is pungent and bitter, sharp and powerful. It is an important medicine for dispelling qi and eliminating mass, eliminating accumulation and guiding stagnation. Wait for the card, often as the main drug. Pharmacological studies have found that Citrus aurantium has the following multiple medicinal uses: It has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07
Inventor 李伟黄华学
Owner HUNAN HUACHENG BIOTECH
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