Method for extracting hesperidin from fructus aurantii immaturus
A technology of hesperidin and citrus aurantium, which is applied in the field of separation and extraction of natural products, can solve the problems affecting the purity of diosmin and drug efficacy in semi-synthetic products, and achieve the effect of consistent process, high content and low production cost
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Embodiment 1
[0050] (1) Degreasing: take 100kg of Citrus citrus raw material, crush it to a particle size of 1-1.5mm, put it into an extraction tank, add petroleum ether, heat and reflux for extraction twice (the first time is 900L, and the extraction time is 2 hours; the second time is 600L , extraction time 2 hours), filtered to obtain extraction slag.
[0051] (2) Enzymolysis: Distill the residual organic solvent in the extraction residue in step (1) with steam, add 900L pure water, adjust the pH value to 5.0, and add 0.2kg cellulase and 0.2kg protopectinase , mix well, and stir at room temperature for 6 hours for enzymatic hydrolysis.
[0052] (3) Impurity removal and centrifugation: heat the material after enzymatic hydrolysis in step (2) to 85° C., stir and extract for 3 hours, cool to room temperature, and centrifugally filter to obtain centrifuge residue.
[0053] (4) Extraction and separation of hesperidin: step (3) centrifugal slag is put into the percolator, and 600L lye is add...
Embodiment 2
[0056] (1) Degreasing: take 100kg of Fructus Citrus citrus raw material, crush it to a particle size of 1-1.5mm, put it into an extraction tank, add 6# solvent oil, heat and reflux for extraction twice (1000L for the first time, 2 hours for extraction time; time 600L, extraction time 1.5 hours), filtered to obtain extraction residue for later use.
[0057] (2) Enzymolysis: Distill the residual organic solvent in the extraction residue in step (1) with steam, add 800L of pure water, adjust the pH value to 5.5, and add 0.1kg of cellulase and 0.2kg of protopectinase , mix well, and stir at room temperature for 5 hours for enzymatic hydrolysis.
[0058] (3) Impurity removal and centrifugation: heat the material after enzymatic hydrolysis in step (2) to 90° C., stir and extract for 3 hours, cool to room temperature, and centrifuge to obtain a centrifuge residue.
[0059] (4) Extraction and separation of hesperidin: step (3) centrifugal slag is put into the percolator, and 500L lye...
Embodiment 3
[0062] (1) Degreasing: take 100kg of Citrus citrus raw material, crush it to a particle size of 1-1.5mm, put it into an extraction tank, add cyclohexane, heat and reflux for extraction twice (the first time is 800L, and the extraction time is 3 hours; the second time 700L, extraction time 2 hours), filtered to obtain extraction residue.
[0063] (2) Enzymolysis: distill the residual organic solvent from the extraction residue in step (1) with steam, add 1000L of pure water, adjust the pH value to 6.0, and add 0.3kg of cellulase and 0.2kg of protopectinase , mix well, and stir at room temperature for 4.5 hours for enzymatic hydrolysis.
[0064] (3) Impurity removal and centrifugation: heat the material after enzymatic hydrolysis in step (2) to 95°C, stir and extract for 2 hours, cool to room temperature, and centrifuge to obtain centrifuge residue.
[0065] (4) Extraction and separation of hesperidin: step (3) centrifugal slag is put into the percolator, and 600L lye is added ...
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