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Application of Pomegranate Peel Flavonoids in the Preparation of Drugs Inhibiting Bacterial Quorum Sensing System

A quorum sensing system and skin flavonoid technology, applied in the field of medicine, can solve the problem of less bacterial quorum sensing system, etc., and achieve the effects of not easy drug resistance, low toxicity and side effects, and preventing bacterial infection

Active Publication Date: 2022-03-22
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A systematic review of the literature on pomegranate peels found that modern pharmacology and clinical studies have also shown that pomegranate peels have strong inhibitory effects on common bacteria such as Pseudomonas aeruginosa, Salmonella bacillus, Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. There are few literature reports on its inhibition of bacterial quorum sensing system

Method used

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  • Application of Pomegranate Peel Flavonoids in the Preparation of Drugs Inhibiting Bacterial Quorum Sensing System
  • Application of Pomegranate Peel Flavonoids in the Preparation of Drugs Inhibiting Bacterial Quorum Sensing System
  • Application of Pomegranate Peel Flavonoids in the Preparation of Drugs Inhibiting Bacterial Quorum Sensing System

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: the preparation of pomegranate peel flavonoids

[0049] (1) Take 100 g of pomegranate peel, soak it in 20 times the amount of 70% (v / v) ethanol overnight, and then extract it 3 times with ultrasonic assistance, the ultrasonic power is 150W, the extraction temperature is 50°C, and each time is 60min to obtain the initial extract;

[0050] (2) filter to remove insoluble matter, and the filtrate is dried under reduced pressure to no alcohol smell, and set aside;

[0051] (3) In the concentrated solution obtained in (2), add 1% gelatin solution until no precipitation occurs, and filter to remove the precipitation;

[0052] (4) After concentrating the filtrate in (3) under reduced pressure to a certain concentration (within 100mL), set aside;

[0053] (5) Take an appropriate amount of D101 macroporous resin, add 1 to 2 times the volume of absolute ethanol to soak overnight, then wet fill the resin, and wash with absolute ethanol until the effluent is 1:1, add ...

Embodiment 2

[0057] Example 2: Effects of flavonoids from pomegranate peel on Pseudomonas aeruginosa PAO1-lasB pathway

[0058] Test sample: take the pomegranate peel flavonoids (GP-OF) of Example 1 and prepare a 100 mg / mL stock solution with 100% DMSO.

[0059] Experimental method: the flavonoid compound of pomegranate peel of the present invention is mixed with ABTGC medium in a 96-well microtiter plate and mixed with a double dilution method to finally have 100 μL of drug-containing ABTGC medium in each well. Get PAO1-lasB-gpf bacterial strain at 37 ℃, grow in the LB medium of 200rpm and be diluted to the final optical density of 600nm (OD600) in ABTGC medium and be 0.02 (2.5 * 10 8 CFU / mL). Next, 100 μL of bacterial suspension was added to each well of a 96-well microtiter plate, so that the final concentration of the compound of the present invention in each well was 100, 50, 25, 12.5, 6.25 3.125, 1.5625 μg / mL. And set 10 μ M / mL isothiocyanate compound Iberin positive control group ...

Embodiment 3

[0061] Example 3: Effect of pomegranate peel on Pseudomonas aeruginosa pqsA pathway

[0062] Test sample: Take pomegranate peel flavonoids from Example 1 and prepare a 100 mg / mL stock solution with 100% DMSO.

[0063] Experimental method: take the cultured PAO1-pqsA-gfp green fluorescent protein reporter strain, and implement the experimental method of Example 2.

[0064] Experimental results: by Figure 4 Pomegranate peel flavonoids inhibited the PAO1-pqsA pathway experiment results showed that the expression level of pqsA-gfp in each group showed a trend of first increasing, then decreasing, and then gradually stabilizing with the increase of culture time; Flavonoids have a significant inhibitory effect on the fluorescence of the strain pqsA-gfp, and there is a dose-effect relationship. High concentrations of pomegranate peel flavonoids have a stronger inhibitory effect on the expression of the pqsA-gfp reporter gene, which is close to that of the positive drug on the lasB-...

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Abstract

The invention relates to the technical field of medicine, in particular to the application of pomegranate peel flavonoids in the preparation of medicines for inhibiting bacterial quorum sensing systems. The invention discloses the application of pomegranate peel flavonoids in the preparation of drugs for inhibiting bacterial quorum sensing system, the application in the preparation of drugs for inhibiting the secretion of bacterial pyocyanin, the application in the preparation of drugs for inhibiting the secretion of bacterial elastase, and the application in the preparation of drugs for inhibiting bacterial growth in the application. According to the experimental results, pomegranate peel flavonoids can effectively inhibit the lasB signaling pathway and pqsA signaling pathway in Pseudomonas aeruginosa, thereby inhibiting the secretion of elastase and pyocyanin, and at the same time inhibiting the growth of Pseudomonas aeruginosa. Further reduce bacterial virulence and pathogenicity, making it difficult for Pseudomonas aeruginosa to develop drug resistance. In addition, pomegranate peel flavonoids are natural substances with low toxicity and side effects. Pomegranate peel flavonoids can be used alone or as an auxiliary therapeutic agent for antibiotics to prevent and treat bacterial infections.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of pomegranate peel flavonoids in the preparation of medicines for inhibiting bacterial quorum sensing systems. Background technique [0002] The use of antibiotics has effectively controlled many serious bacterial infectious diseases in humans, but the widespread use or even abuse of antibiotics has also led to the increase of drug-resistant strains and reduced the antibacterial efficiency of existing antibiotics. At present, antibiotic resistance has become a serious public health problem worldwide. Therefore, it is possible to develop drugs that do not kill pathogenic bacteria cells but only weaken the virogenicity of pathogenic bacteria, which will be able to solve the problem of antibiotic resistance well. [0003] Bacterial quorum sensing (QS) system is a way of intracellular or intercellular signal transmission in bacteria. Microbes release certain signal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61K36/185A61P31/04
CPCA61K36/185A61P31/04
Inventor 郑俊霞陈鸿庚黄智霖谢俊涛王立抗黄家晋苏亚伦孙平华黎相广杨超田文月吴可凌华英
Owner GUANGDONG UNIV OF TECH
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