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A passive micro-mixer for chemiluminescent detection and method of use thereof

A chemiluminescence detection and micro-mixer technology, which is applied in the fields of microfluidic technology and biochemical detection, can solve the problems such as the inability to achieve the strongest luminescence intensity, the complex structure of the microfluidic chip, and the increase in the length of the detection channel, and the solution mixing process can be achieved. The effect of high efficiency, shortened mixing distance, and shortened detection distance

Active Publication Date: 2021-10-15
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The reported microfluidic chips related to chemiluminescence detection have the following disadvantages: 1. The channel structure of the microfluidic chip is linear, and the sample is not mixed enough to achieve the strongest luminous intensity. For example, the patent application number is CN201610517808.6 A microfluidic chip for chemiluminescence detection and its detection method disclosed in the Chinese patent; 2. The structure of the microfluidic chip is complex, which increases the difficulty of manufacturing. chemiluminescence microfluidic chip; 3. the detection channel is helical, and the sample injection mixing distance is long, which increases the length of the detection channel. Fluidic chip

Method used

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  • A passive micro-mixer for chemiluminescent detection and method of use thereof
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  • A passive micro-mixer for chemiluminescent detection and method of use thereof

Examples

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Effect test

Embodiment 1

[0040] A cross-type passive micromixer for chemiluminescent detection, such as figure 1 As shown, it includes a micro-mixer matrix 1 and a cross-shaped channel arranged inside it. The four directions of the cross-shaped channel are: the sample channel 4 to be tested, the first sheath flow channel 6-1, the detection channel 10 and the second channel. The outflow end of the sheath flow channel 6-2, the sample channel 4 to be tested, the first sheath flow channel 6-1, and the second sheath flow channel 6-2 are connected to the inflow end of the detection channel 10 through the mixing chamber 2, and the mixing chamber 2 is connected to the detection channel 10 through a narrow connection channel 9, the outflow end of the detection channel 10 is connected to the waste liquid pool 8 through the waste liquid outflow channel 7, and the inflow end of the sample channel 4 to be tested is connected to the sample liquid storage pool to be tested 3, the inflow ends of the first sheath flow...

Embodiment 2

[0047] A Y-type passive micro-mixer for chemiluminescence detection, comprising a micro-mixer base 1 and a Y-shaped channel arranged inside it, such as Figure 5 As shown, the three directions of the Y-shaped channel are: the sample channel 4 to be tested, the sheath flow channel 6, the detection channel 10, the sample channel 4 to be tested, the outflow end of the sheath flow channel 6 and the inflow end of the detection channel 10 Connected through the mixing chamber 2, the mixing chamber 2 and the detection channel 10 are connected through a narrow connection channel 9, the outflow end of the detection channel 10 is connected to the waste liquid pool 8 through the waste liquid outflow channel 7, and the inflow of the sample channel 4 to be tested The end is connected with the sample storage tank 3 to be tested, and the inflow end of the sheath flow channel 6 is connected with the sheath flow storage tank 5 .

[0048] The detection channel 10 is composed of more than three m...

Embodiment 3

[0053] A method for using the cross-type passive micro-mixer of embodiment 1, adopting the cross-type passive micro-mixer of embodiment 1 to carry out chemiluminescence detection transition metal cobalt ion concentration, comprising the steps:

[0054] (1) To clean the flow path, use 1.0mol L sequentially -1 Wash with NaOH for 30 minutes, then wash with ultrapure water for 5 minutes;

[0055] (2) The sample storage tank 3 to be tested, the first sheath flow storage tank 5-1, and the second sheath flow storage tank 5-2 are respectively used to hold the cobalt chloride solution to be tested, the hydrogen peroxide solution and the Minol solution;

[0056] Negative pressure is applied to the waste liquid tank 8, or positive pressure is applied to the sample storage tank 3 to be tested, the first sheath flow storage tank 5-1, and the second sheath flow storage tank 5-2 to drive the cobalt chloride solution , hydrogen peroxide solution and luminol solution move to the detection ch...

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Abstract

The invention discloses a passive micro-mixer for chemiluminescence detection, which comprises a micro-mixer base body and a sample channel to be tested, a sheath flow channel and a detection channel arranged inside the micro-mixer, and outlets of the sample channel to be tested and the sheath flow channel The flow end and the inflow end of the detection channel are connected through a mixing chamber. The detection channel is composed of several mixing units in series. Each mixing unit is equipped with a Z-shaped microstructure baffle, and the Z-shaped microstructure baffle is connected to the mixing unit. There is a gap between the side walls of the channel, and the Z-shaped microstructure baffles in two adjacent mixing units are mirror-symmetrically arranged. The passive micro-mixer can effectively improve the mixing quality, shorten the mixing distance, enhance the intensity of chemiluminescence, and further improve the sensitivity of chemiluminescence detection, has a simple structure and has wide application prospects. The invention also discloses the use method of the passive micro-mixer, which is easy to operate and can quickly and accurately perform chemiluminescence detection.

Description

technical field [0001] The invention belongs to the technical fields of microfluidic technology and biochemical detection, and in particular relates to a passive micro-mixer for chemiluminescent detection and a method for using the same. Background technique [0002] Microfluidics is a new technology for manipulating particles and fluids at the micron or submicron scale. As an important carrier of microfluidic technology, microfluidic chips can realize a series of operations such as sample injection, pretreatment, mixing, reaction, and detection through structures such as microchannels, microvalves, and micropumps, which greatly shortens sample processing time and facilitates detection. The resolution is significantly improved. At present, microfluidic chips are widely used in the fields of disease diagnosis, environmental monitoring, and food science due to their advantages of integration, miniaturization, and low consumption, and have great development potential and appli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01F13/00G01N1/38G01N21/76
CPCG01N21/76G01N1/38B01F33/3017
Inventor 蒋炳炎袁帅周明勇
Owner CENT SOUTH UNIV
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