A kind of vancomycin-modified black phosphorus quantum dot antibacterial agent, preparation method and application
A technology of vancomycin and vancomycin hydrochloride, which is applied in the direction of antibacterial drugs, skin care preparations, medical preparations of non-active ingredients, etc., can solve the problems of high cost, toxic and side effects, long cycle, etc., and achieve improved antibacterial Effect, excellent optical performance, good effect of biocompatibility
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[0033] Preferably, the specific steps of the preparation method are as follows: dissolving vancomycin hydrochloride and N-hydroxysuccinimide in DMSO, cooling the solvent and adding 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride (EDC), mixed and placed in an ice bath, and continued to add N-BoC-(ethylenedioxy)diethylamine and N,N-diisopropylethylamine (DIPEA) Stir overnight, add acetone and centrifuge to obtain the precipitated part; obtain the sample by liquid phase separation, and treat the sample with a mixture of dichloromethane, trifluoroacetic acid and diethyl ether in turn; add black phosphorus quantum dot solution, adjust the pH to 7~ 8 Obtain the vancomycin-modified black phosphorus quantum dots.
[0034] Preferably, the precipitation part is sequentially treated with a mixture of dichloromethane, trifluoroacetic acid and diethyl ether as follows: the sample is placed in a mixture of dichloromethane and trifluoroacetic acid and stirred under ice bath cond...
Embodiment 1
[0051] (1) 100 mg of black phosphorus crystals were ground into powder and stored in 100 ml of N-methylpyrrolidone (NMP) solution under the protection of nitrogen. Take 10ml of black phosphorus NMP solution and sonicate for 15min. After the solution is mixed evenly, add 10ml of black phosphorus NMP solution, 90ml of NMP, and 100mg of NaOH to a 250ml conical flask, and stir for 6h under the protection of nitrogen at 140°C. . After the reaction was completed, it was cooled to room temperature. The reacted solution was centrifuged at 7000r for 20min to obtain bottom black phosphorus and supernatant. The supernatant was then centrifuged at 15100r for 20min to obtain the supernatant black phosphorus. The supernatant black phosphorus was washed 3 times and air-dried naturally to obtain the black phosphorus quantum dot antibacterial agent. Its TEM image is as attached. figure 1 shown.
[0052] (2) The bacterial culture solution is prepared in the ratio of 10g / L of sodium chloride,...
Embodiment 2
[0055] (1) with step (1) in embodiment 1.
[0056] (2) Weigh vancomycin hydrochloride 0.1040 g (70 μmol, 1 equiv.), N-methylsuccinimide (84 μmol, 1.2 equiv.) 0.0097 g, dissolve in 2 ml of dry DMSO, and place the mixture solution in ice water The mixture was cooled to 0° C., EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 84 μ mol, 1.2 equivalents) 0.161 g was added, stirred for 30 min, and put into ice water. N-BoC-(ethylenedioxy)diethylamine (77 μmol, 1.1 equiv.) 0.0191 g and DIPEA (860 μmol, 2.4 equiv.) 0.15 mol were added to the mixture, followed by stirring overnight. The solution stirred overnight was placed in a centrifuge tube, acetone was added, centrifuged for 10 min, the supernatant was taken out, sealed and placed in the refrigerator, and the precipitate was also sealed and placed in the refrigerator for refrigeration. The precipitates in the refrigerator and refrigerator were dissolved with chromatographic methanol, the supernatant was taken out ...
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