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Application of Tibetan medicine namely epipremnum aureum flower in preparation of medicine for treating atherosclerosis

A technology of atherosclerosis and green dill flower, which is applied in the field of preparation of anti-atherosclerosis drug, Tibetan medicine green dill flower, which can solve the problems of affecting the clinical treatment effect, large toxic and side effects, and patients' intolerance, so as to promote plaque Stability, low cost of raw materials, no adverse reactions

Inactive Publication Date: 2020-11-03
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the defects of the existing medicines for treating atherosclerosis, which have large toxic and side effects, patients cannot tolerate them, and affect the clinical treatment effect, and provide a new application of pothos chinensis in pharmacy, specifically relating to the use of pothos osmanthus in the preparation of therapeutic arteriosclerosis. Use in atherosclerotic drugs

Method used

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  • Application of Tibetan medicine namely epipremnum aureum flower in preparation of medicine for treating atherosclerosis
  • Application of Tibetan medicine namely epipremnum aureum flower in preparation of medicine for treating atherosclerosis
  • Application of Tibetan medicine namely epipremnum aureum flower in preparation of medicine for treating atherosclerosis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1. The preparation of the pothos alcohol extract

[0025] Weigh 500g of the dried flower buds of the green dill flower, and put them into two 5000ml flasks equally; add 4000ml of 60% ethanol to each flask. Use a temperature-controlled electric heating mantle to heat the flask, collect 8000ml of the extract 2 hours after the liquid boils; add 4000ml of 60% ethanol to each flask and continue to decoct for 2 hours; collect a total of 16000ml of ethanol extract twice, spin dry and concentrate Collect and evaporate to dryness in a water bath at 80°C; weigh and store in a refrigerator at 4°C. Commonly used human dose (g / d) / normal human body weight (60kg)×9.1=mouse dose (g / d.bw), generally 9g of Chinese herbal medicine is the maximum safe dose, converted to 2g (crude drug) / kg (body weight) For the middle dose group of mice gavage, dilute the drug by 2 times to set up three concentrations of low, middle and high (1g / kg, 2g / kg, 4g / kg) gavage dose groups, and use ster...

Embodiment 2

[0026] Example 2. Analysis of the atherosclerotic lesions of the full-length aorta by the alcohol extract of the pothos radish

[0027] Male ApoE- / - mice (provided by Nanjing University-Nanjing Institute of Biomedicine) were randomly divided into 4 groups from 9 weeks old, high fat and high cholesterol diet (high fat diet, HFD), high fat and high Cholesterol + green dill flower low dose group (HFD+1g / kg EG), high fat and high cholesterol + green dill flower medium dose group (HFD+2g / kg EG), high fat and high cholesterol + green dill flower high dose group (HFD+4g / kg / kg EG), and each group was given a HFD in which the proportion of fat energy supply to the total energy was 42%. The pothos flower-administered group received different concentrations of ethanol extracts from the pothos flower by intragastric administration every day, and the intragastric administration dose was 10ml liquid medicine / kg mouse body weight, and continued intragastric administration for 16 weeks.

[...

Embodiment 3

[0030] Example 3. The influence of the alcohol extract of the pothos flower on the plaque area of ​​the aortic root

[0031] The mouse heart was cut transversely from the top to the middle of the apex, and the upper half was fixed in 4% PFA for 24 hours; washed with running water for 4 hours; dehydrated overnight in 30% sucrose solution at 4°C refrigerator; embedded in frozen section embedding medium (OCT) Tissue; cryosection after quick-freezing in liquid nitrogen; the top of the mouse heart faces outward, and the thickness of the slice is 10 μm. Slices were collected serially until no valve appeared at the aortic root.

[0032]Hematoxylin and eosin staining was performed on the slices, and the steps were as follows: wash off the OCT embedding agent with PBS solution; infiltrate with pure water for 5 minutes; stain the nucleus with Harris hematoxylin staining solution for 3 minutes; wash away excess dyeing solution with running water; differentiate with 1% hydrochloric acid a...

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Abstract

The invention relates to an application of a Tibetan medicine namely epipremnum aureum flower in preparation of a medicine for treating atherosclerosis, and belongs to the technical field of traditional Chinese medicine. The invention shows that at the animal level, the epipremnum aureum flower alcohol extract can significantly reduce the atherosclerotic plaque area of mice, and the specific mechanism is shown as follows: on one hand, cholesterol absorption is inhibited by reducing the SR-BI expression level, and on the other hand, cholesterol metabolism is promoted to generate bile acid by increasing the CYP7A1 gene expression; and at the cellular level, the epipremnum aureum flower alcohol extract can significantly reduce cholesterol uptake of cells and reduce expression of a macrophagemarker CD36 to reduce cholesterol uptake of macrophages so as to reduce macrophage foaming. The invention provides scientific data and an idea basis for clinical treatment of atherosclerosis through the epipremnum aureum flower, and provides data support for a new method and an action target for clinically preventing and treating cardiovascular diseases.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to the use of Tibetan medicine Poulodron in the preparation of medicines for treating atherosclerosis. Background technique [0002] With the development of modern technology and the accelerated pace of work and life, chronic diseases (such as cardiovascular disease, diabetes, etc.) diseases such as cancer and diabetes. According to WHO statistics, cardiovascular disease will affect 23.6 million people worldwide by 2030. According to the "2018 China Cardiovascular Disease Report", 2 out of every 5 deaths in my country died of cardiovascular disease, and the incidence rate is gradually increasing, and there is a trend of younger age. [0003] Atherosclerosis (AS) is the main pathological basis of cardiovascular disease and the main cause of death in patients with cardiovascular disease. It refers to the progressive pathological process characterized by the da...

Claims

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Application Information

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IPC IPC(8): A61K36/888A61P9/10A61K133/00
CPCA61K36/888A61K2236/333A61P9/10
Inventor 余军裘梁钟国跃唐乐秦嫚嫚
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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