Application of Tibetan medicine namely epipremnum aureum flower in preparation of medicine for treating atherosclerosis
A technology of atherosclerosis and green dill flower, which is applied in the field of preparation of anti-atherosclerosis drug, Tibetan medicine green dill flower, which can solve the problems of affecting the clinical treatment effect, large toxic and side effects, and patients' intolerance, so as to promote plaque Stability, low cost of raw materials, no adverse reactions
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Embodiment 1
[0024] Embodiment 1. The preparation of the pothos alcohol extract
[0025] Weigh 500g of the dried flower buds of the green dill flower, and put them into two 5000ml flasks equally; add 4000ml of 60% ethanol to each flask. Use a temperature-controlled electric heating mantle to heat the flask, collect 8000ml of the extract 2 hours after the liquid boils; add 4000ml of 60% ethanol to each flask and continue to decoct for 2 hours; collect a total of 16000ml of ethanol extract twice, spin dry and concentrate Collect and evaporate to dryness in a water bath at 80°C; weigh and store in a refrigerator at 4°C. Commonly used human dose (g / d) / normal human body weight (60kg)×9.1=mouse dose (g / d.bw), generally 9g of Chinese herbal medicine is the maximum safe dose, converted to 2g (crude drug) / kg (body weight) For the middle dose group of mice gavage, dilute the drug by 2 times to set up three concentrations of low, middle and high (1g / kg, 2g / kg, 4g / kg) gavage dose groups, and use ster...
Embodiment 2
[0026] Example 2. Analysis of the atherosclerotic lesions of the full-length aorta by the alcohol extract of the pothos radish
[0027] Male ApoE- / - mice (provided by Nanjing University-Nanjing Institute of Biomedicine) were randomly divided into 4 groups from 9 weeks old, high fat and high cholesterol diet (high fat diet, HFD), high fat and high Cholesterol + green dill flower low dose group (HFD+1g / kg EG), high fat and high cholesterol + green dill flower medium dose group (HFD+2g / kg EG), high fat and high cholesterol + green dill flower high dose group (HFD+4g / kg / kg EG), and each group was given a HFD in which the proportion of fat energy supply to the total energy was 42%. The pothos flower-administered group received different concentrations of ethanol extracts from the pothos flower by intragastric administration every day, and the intragastric administration dose was 10ml liquid medicine / kg mouse body weight, and continued intragastric administration for 16 weeks.
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Embodiment 3
[0030] Example 3. The influence of the alcohol extract of the pothos flower on the plaque area of the aortic root
[0031] The mouse heart was cut transversely from the top to the middle of the apex, and the upper half was fixed in 4% PFA for 24 hours; washed with running water for 4 hours; dehydrated overnight in 30% sucrose solution at 4°C refrigerator; embedded in frozen section embedding medium (OCT) Tissue; cryosection after quick-freezing in liquid nitrogen; the top of the mouse heart faces outward, and the thickness of the slice is 10 μm. Slices were collected serially until no valve appeared at the aortic root.
[0032]Hematoxylin and eosin staining was performed on the slices, and the steps were as follows: wash off the OCT embedding agent with PBS solution; infiltrate with pure water for 5 minutes; stain the nucleus with Harris hematoxylin staining solution for 3 minutes; wash away excess dyeing solution with running water; differentiate with 1% hydrochloric acid a...
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