Method and apparatus for constructing blood vessel in vitro in the tissue project

A technology of tissue engineering and construction method, which is applied in the direction of tissue cell/virus culture device, tissue culture, biochemical cleaning device, etc. It can solve the problems of inability to achieve the structural strength of blood vessels, lack of elastic medial support of blood vessels, and shedding of endothelial cell layer, etc. problem, to achieve the effect of simple structure, promotion of directional arrangement, and increase of shrinkage characteristics
CN1236056CInactive Publication Date: 2006-01-11上海组织工程研究与开发中心
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
上海组织工程研究与开发中心
Publication Date
2006-01-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses the method and apparatus for externally constructing blood vessel tissue engineering. Biomechanical factors are applied to stimulate proliferation of blood vessel smooth muscle, promote directional arranging of blood vessel, increase the contraction characteristic of smooth muscle cell, increase the collagen content in cell-material composition and the expression of actin heavy strand in cell and induce endothelial cell to form stress fiber via stress. The apparatus of the present invention includes reactor and controller and the controller has its signal port connected via wire to solenoid valve to form pulsed water flow and flow rate to simulate internal physiological environment. The apparatus has reasonable structure, stable performance and easy operation, and can perform data processing and analysis can provide enclosed, non-toxic, bacteria-free and oxygen-rich environment for external blood vessel construction.
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Description

technical field

[0001] The invention relates to an in vitro construction method of tissue engineered blood vessels and a construction device adopted, in particular to an in vitro construction method and a bioreactor capable of simulating the pulsation stimulation of human blood vessels and the flushing effect of blood flow. Background technique

[0002] With the increase in the incidence of hypertension, diabetes, etc., the incidence of peripheral vascular disease is increasing year by year. There are more than 1 million patients who need vascular transplantation due to vascular disease, and 60% of them require small blood vessels with a diameter of less than 6mm. People try Autologous arteries and veins, allografts, xenograft arteries and veins, artificial blood vessel endothelialization and other methods. However, these methods have more or less deficiencies in terms of long-term effects. The concept of tissue engineering in the late 1980s and its rapid development inject...

Claims

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