Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rare earth-DNA photo-thermal hydrogel for injection as well as preparation method and application thereof

A technology for injection and DNA molecules, which is applied to medical preparations without active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, etc., which can solve the problem of weakening the effect of photothermal therapy at tumor sites, and the difficulty of photothermal treatment of UBC. The complex fluid environment and other problems can achieve the effect of high-efficiency photothermal treatment, low tumor recurrence and metastasis, and simple and fast operation.

Active Publication Date: 2020-11-06
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional treatment mode of tail vein injection, the cumulative concentration of nanomaterials at the tumor site is low, which greatly weakens the effect of photothermal therapy on the tumor site
In addition, the circulation of photothermal materials in the body will have certain toxic and side effects on normal tissues and organs.
In particular, some nanomaterials pose long-term risks to organisms due to their non-degradability
In addition, due to the complex fluid environment in the urothelial bladder, it is difficult to treat UBC with photothermal agents

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rare earth-DNA photo-thermal hydrogel for injection as well as preparation method and application thereof
  • Rare earth-DNA photo-thermal hydrogel for injection as well as preparation method and application thereof
  • Rare earth-DNA photo-thermal hydrogel for injection as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0046] The present invention does not limit the preparation method of the UCNP nanoparticles, and methods well-known to those skilled in the art will suffice.

[0047] Preferably specifically: YCl 3 , YbCl 3 , ErCl 3 , oleic acid and octadecene were mixed, stirred, heated to 140-160°C under nitrogen protection, maintained for 30-40min, and then lowered to 40-50°C to obtain the first mixed solution;

[0048] Take NH 4 F and NaOH were dissolved in methanol, after ultrasonic mixing, mixed with the first mixed solution, stirred at 50°C for 1-2 hours, then evaporated to remove the solvent, washed to obtain solution A;

[0049] YCl 3 , NdCl 3 Mix oleic acid and octadecene, stir, raise the temperature to 160°C under nitrogen protection, maintain it for 30-40min, and then lower it to 40-50°C to obtain the second mixed solution;

[0050] Take NH 4 Dissolve F and NaOH in methanol, ultrasonically mix, heat and stir with the second mixed liquid and solution A, heat up to evaporate ...

Embodiment 1

[0091] The present invention provides a hydrogel with good biocompatibility, injectability, remarkable photothermal conversion effect and photodynamic effect, which is composed of negatively charged DNA molecules—salmon sperm DNA and UCNP with a large number of positive charges - Made of Au nanoparticles, the specific preparation process is as follows:

[0092] 1. Synthesis of UCNP-Au nanoparticles with a large number of positive charges:

[0093] The first step: prepare the core-shell type hexagonal phase-rare earth doped fluorescent upconversion nanomaterial (βOA-UCNP) by high temperature oil solvent method. The details are as follows: Take 152.3mg of YCl3, 55.88mg of YbCl3 and 5.47mg of ErCl3 into a 100mL round bottom flask, then add 6ml of oleic acid and 18ml of octadecene, and keep stirring. After exhausting the gas, the temperature was raised to 160°C for 40 minutes under the protection of nitrogen, and then lowered to 50°C. Take 149mg NH 4 F and 100mg NaOH were disso...

Embodiment 2

[0103] Example 2 is similar to Example 1, except that the mass fraction ratio of salmon sperm DNA (2000bp) to UCNP-Au nanoparticles is 15:1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides rare earth-DNA photo-thermal hydrogel for injection. The hydrogel comprises DNA molecules with negative charges and a rare earth-based nano material with positive charges; and the rare earth-based nano material with the positive charges is UCNP-Au. The DNA molecules and the rare earth-based nano material form a hydrogel material with injectability, efficient photothermal therapy effect and biocompatibility under the action of physical winding, hydrogen bonds and electrostatic force. Experimental results show that the photo-thermal efficiency of the injectable rare earth-DNA photo-thermal hydrogel disclosed by the invention can reach 42.67%. In addition, the tumor inhibition rate is increased, so that the recurrence and metastasis probability of tumors is reduced. Toxic and side effects of medicines on normal tissues in the treatment process are reduced through a paratumoral injection means. Moreover, the degradation of the photo-thermal hydrogel in any time period can be realized by regulating and controlling the ratio of the DNA molecules to the rare earth-based nano material. The hydrogel is simple and rapid to operate and low in tumor recurrence and metastasis probability.

Description

technical field [0001] The invention relates to the technical field of self-assembly of natural biological macromolecules and emerging rare earth nanocomposite materials, in particular to an injectable rare earth-DNA photothermal gel, its preparation method and application. Background technique [0002] Urothelial bladder carcinoma (UBC) is the most common malignant tumor in the urinary system, among which non-muscle-invasive type is the most common. For this type of bladder cancer, transurethral resection of bladder tumor (TURBT) has become the most important treatment because of its simplicity and ease of operation. However, due to incomplete resection, there is a high rate of tumor recurrence after surgery, and regular intravesical infusion of chemotherapy drugs is often required. In addition, the frequent application of chemotherapy drugs will not only cause great side effects on the human body, but also lead to the increase of drug resistance, which is extremely unfavo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K9/06A61K47/26A61K41/00A61P35/00
CPCA61K9/0019A61K9/06A61K41/0052A61K47/26A61P35/00
Inventor 刘凯刘斌王帆张洪杰
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com