Bioengineering protein plaster as well as preparation method and application thereof

A bioengineering and protein technology, applied in pharmaceutical formulations, drug combinations, antitumor drugs, etc., can solve the problems of increasing the incidence of complications, inconvenient daily operations, etc., achieving strong adhesion performance, simple and fast treatment methods, and reducing The effect of tumor recurrence

Pending Publication Date: 2022-07-08
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, external injection is still inconvenient for daily operation and increases the incidence of complications

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bioengineering protein plaster as well as preparation method and application thereof
  • Bioengineering protein plaster as well as preparation method and application thereof
  • Bioengineering protein plaster as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0040] In the present invention, the preparation method of the smearable bioengineered protein plaster provided by the present invention includes:

[0041] A) adding positively charged elastin into deionized water and mixing evenly to obtain a homogeneous protein solution;

[0042] B) adding the negatively charged anionic surfactant to deionized water, and mixing uniformly to obtain a homogeneous surfactant solution;

[0043] C) adding the gold nanorod solution to the protein solution, shaking and mixing evenly, then adding the surfactant solution, shaking and centrifuging to obtain a bioengineered protein plaster.

[0044] In the above preparation method provided by the present invention, the types of elastin-like and alkyl-based surfactants are the same as those mentioned above, and will not be repeated here.

[0045] Further, the concentration of the elastin-like solution is preferably 100-300 mg / mL, specifically 100 mg / mL, 200 mg / mL or 300 mg / mL.

[0046] The concentrati...

Embodiment 1

[0064] Example 1: Expression and purification of K144cys

[0065] Step 1: Obtain a strain stably expressing K144cys. The pET25b prokaryotic expression vector was constructed with the gene sequence of K144cys protein, and then transformed into E.coli BLR (DE3) E. coli, induced expression and screened to obtain a stable expression strain.

[0066] Step 2: Expression of K144cys. The strains were cultured in LB medium and then inoculated into TB medium. After reaching the exponential growth phase, IPTG was added to induce protein expression at 28.5 °C for 12 h, and the cells were washed and collected by centrifugation, and stored at -80 °C.

[0067] Step 3: Purification of K144cys. After the bacteria were crushed by high pressure, the supernatant was collected by centrifugation, filtered and sterilized, and finally purified by a nickel affinity chromatography column, a cation exchange chromatography column, and a desalting column with a protein purifier to finally obtain the K14...

Embodiment 2

[0068] Example 2: Synthesis of Gold Nanorods

[0069] The first step: prepare a gold nanorod growth solution, as follows: Weigh 7g CTAB and 1.234g sodium oleate, add them to a 1L glass flask, add 250mL deionized water, and heat to 50°C in an oil bath to stir and dissolve . After it was completely dissolved, the temperature of the oil bath was adjusted to 30°C for stabilization. Prepare 24mL AgNO 3 An aqueous solution (concentration of 4 mM) was added to the above glass flask and allowed to stand for 15 min. Prepare 250mL HAuCl 4 The aqueous solution (concentration of 1 mM) in a volumetric flask was added to the above glass flask and stirred for 1 h 30 min.

[0070] The second step: prepare the gold seed solution, as follows: prepare 5 mL of CTAB aqueous solution (concentration of 0.2 mM) in a 100 mL glass flask, and place it in a 30° C. oil bath to keep warm. Prepare 5mL of HAuCl 4 An aqueous solution (0.5 mM concentration) was added to the above 100 mL glass flask and s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Concentrationaaaaaaaaaa
Login to view more

Abstract

The invention relates to the technical field of biological materials, in particular to a bioengineering protein plaster and a preparation method and application thereof, and the bioengineering protein plaster is composed of elastin-like molecules with positive charges, gold nanorods and alkyl surfactants with negative charges. Experiments show that the bioengineering protein plaster is evenly smeared on the surface of a tumor, then photothermal conversion is generated through irradiation, the tumor can be effectively killed, and tumor recurrence is reduced. Compared with an existing clinical non-surgical tumor treatment means, the anti-tumor effect is good, the tumor recurrence and metastasis probability is low, the treatment method is simple, rapid and easy to operate, and the treatment strategy will provide a new thought for clinical non-surgical skin-related malignant tumor treatment.

Description

technical field [0001] The invention relates to the technical field of biological materials, in particular to a biological engineering protein plaster and a preparation method and application thereof. Background technique [0002] Melanoma is the deadliest malignancy among skin cancers and has become one of the fastest growing tumors in recent years. Due to the invasion and migration ability of malignant melanoma, traditional surgical therapy is difficult to remove the entire tumor tissue and cannot avoid the high risk of tumor recurrence. For emerging targeted therapies and immunotherapies, drug resistance and low immunotherapeutic activity are major challenges. [0003] Photothermal therapy (PTT) has attracted widespread attention in cancer treatment due to its minimal damage to tissues and low invasiveness. Especially for skin tumors like melanoma, near-infrared (NIR) light-based photothermal therapy has the potential advantage of a large tissue penetration depth. Howe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K9/70A61K41/00A61K47/64A61K47/54A61P35/00
CPCA61K41/0052A61K9/7015A61P35/00A61K47/64A61K47/54Y02A50/30
Inventor 刘凯王帆位政张洪杰
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap