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High-potential hydrophobic polypeptide monolayer film as well as preparation method and application thereof

A single-layer membrane, hydrophobic technology, applied in the field of natural polymers, can solve problems such as limiting the application of polypeptide molecules, and achieve the effect of improving cell adhesion and proliferation, and improving biocompatibility

Active Publication Date: 2020-11-06
QILU UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the use of surfactants to regulate the conformation of polypeptide molecules on the interface is relatively common, but due to the complexity of the structure of natural biological macromolecules, there are few reports on the chemical properties of the surface of polypeptide molecular monolayer membranes, which limits the ability of peptide molecules. molecular application

Method used

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  • High-potential hydrophobic polypeptide monolayer film as well as preparation method and application thereof
  • High-potential hydrophobic polypeptide monolayer film as well as preparation method and application thereof
  • High-potential hydrophobic polypeptide monolayer film as well as preparation method and application thereof

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Embodiment 1

[0051] Embodiment 1 A method for preparing a polypeptide monolayer film, comprising the following steps:

[0052] (1) Prepare 50 mL of collagen peptide solution with a concentration of 4% wt: accurately weigh 100 mL of collagen peptide in a three-necked flask, accurately measure deionized water, pour the deionized water into the three-necked flask, and swell at room temperature for 0.5 h. Put the three-neck flask in a water bath at 50±1°C, heat and stir for 2 hours to dissolve it completely, then adjust the pH of the solution to 10.00±0.02 with 2mol / L sodium hydroxide, and stabilize it in the water bath for 0.5 hours.

[0053] (2) add surfactant SDS to above-mentioned collagen polypeptide solution, obtain collagen polypeptide-SDS mixed solution, the concentration of SDS in mixed solution is 7.50mmol / L (CMC, when 50 ℃, the critical micelle concentration of SDS); Stable in a water bath for 6h and set aside.

[0054] (3) Cut a rectangular titanium sheet with a size of 1cm×1cm×1m...

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Abstract

The invention provides a polypeptide monolayer film with high surface potential and hydrophobicity as well as preparation and application thereof. The polypeptide is composed of polypeptide moleculeswith a molecular weight of (1.48+ / -0.2) x 10<5> g / mol, the thickness of the monolayer film is 17.3-18.5 nm, the exposure amount of primary amino groups on the surface of the film is 11-11.8%, and theZeta potential of the polypeptide monolayer film is -3 mV to -2 mV. The contact angle of the film is 84+ / -1 degrees. The surface of the polypeptide monolayer film has a micro-nano structure, so that the film has a certain hydrophobic property, and biological bionic skin surface waterproofing is facilitated. The surface of the polypeptide monolayer film has higher potential, so that the biocompatibility, the blood compatibility and the adhesion, proliferation and differentiation capabilities of cells can be improved.

Description

technical field [0001] The invention belongs to the field of natural polymers, and relates to a polypeptide monolayer film and its preparation method and application, in particular to a polypeptide monolayer film with high surface potential and hydrophobicity, its preparation method and application. Background technique [0002] Collagen peptide is a water-soluble protein obtained by chemical thermal degradation of collagen. It is one of the most commonly used biopolymers due to its excellent biocompatibility, plasticity, viscosity, abundance and low cost. As a biodegradable and renewable resource, collagen peptides are widely used in the preparation of medical materials, bionic materials, packaging and coating materials. Bio-immobilization coatings are often used in the field of biomimetic scaffolds to solve the problem of carrying biomolecules such as enzymes, lactose, and polydopamine; drug molecules; synthetic polymers or small organic molecules. The loading capacity i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/24A61L27/18A61L27/60A61L27/50
CPCA61L27/22A61L27/24A61L27/18A61L27/60A61L27/50A61L2430/34C08L89/00C08L79/02A61L2400/12A61L27/227A61L27/48
Inventor 许静班青李天铎张震马慧君
Owner QILU UNIV OF TECH
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