Microbial agent for sweet potato seedling culture and rapid propagation as well as preparation method and application thereof

A microbial inoculum and a technology for raising sweet potato seedlings are applied in the field of agricultural cultivation to achieve the effects of reducing the amount of raw materials used, improving the quality of sweet potato seedlings, and promoting growth.

Inactive Publication Date: 2020-11-06
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, probiotics have not been applied to sweet potato seedling cultivation and propagation. In view of the above-mentioned problems and characteristics of sweet potato planting, it is necessary to select lactic acid b

Method used

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  • Microbial agent for sweet potato seedling culture and rapid propagation as well as preparation method and application thereof
  • Microbial agent for sweet potato seedling culture and rapid propagation as well as preparation method and application thereof
  • Microbial agent for sweet potato seedling culture and rapid propagation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0040] Example 1

[0041] Isolation and purification of (a) strains

[0042] Commercially available milk collected near Qingdao Victory Bridge water and sea Qingdao Sifang Square piano, joined by volume ratio of 1:10 in sterile water to mix serial dilutions, the dilution of the various bacteria were coated with 100μL cloth MRS solid medium, 37 ℃ anaerobic culture 48h; larger clear zone picked single colonies on MRS solid medium chain line three times to give pure consecutive single colonies; Individual colonies were picked to no addition of calcium carbonate the MRS liquid medium, it was observed whether gas production, gas production strain heterofermentative lactic acid bacteria, gas is not producing strain of homofermentative lactic acid bacteria; were isolated from 17 purified isotype and heterofermentative lactic acid bacteria strains; these strains access MRS liquid medium, 37 [deg.] C for 20 h culture, bacteria and whichever of 700μL 300μL sterile glycerol was added, and th...

Example Embodiment

[0066] Example 2

[0067] Hemoglobin increase pentosaceus WS3 density and proliferation of YN12 bacteria Weissella.

[0068] Activation of lactic acid bacteria: Remove stored under -20 ℃ pentosaceus strains Weissella and WS3 YN12, 5μL injection were taken on MRS solid medium, coating, 10 ~ 12h culture at a temperature of 37 [deg.] C; inoculated with Individual colonies were picked ring on MRS solid medium, inoculated to a test tube containing 10mL 5mLMRS liquid medium, and cultured at a temperature of 37 ℃ 8 ~ 10h obtained activated lactic acid bacteria in this case, per ml MRS broth comprising at least 1 × 10 7 cfu lactic acid bacteria.

[0069] Pentosaceus WS3 YN12 and co-cultured bacteria Weissella:

[0070] The above-described lactic acid bacteria culture fluid by two cfu ratio of 1: 2 were mixed together to obtain seed culture liquid of the strain, inoculation amount of 10%, after mixing the liquid was inoculated in the seed-free calcium carbonate , heme-free MRS liquid mediu...

Example Embodiment

[0071] Example 3

[0072] Preparation and Application of microbial agents

[0073] (1) Activation of lactic acid bacteria: Pediococcus pentosaceus WS3 taken under the conditions of storage -20 ℃ above Example 1 and screened bacteria Weissella YN12, 5μL injection were taken on MRS solid medium is applied, at a temperature of 37 cultured under conditions ℃ 10 ~ 12h; picked with an inoculating loop of a single colony on solid MRS medium, inoculated to a test tube containing 10mL 5mLMRS liquid medium, and cultured at a temperature 37 ℃ 10h obtained activated lactic acid bacteria, in this case, per ml MRS liquid medium containing at least 1 × 10 7 cfu lactic acid bacteria.

[0074] (2) Preparation of microbial agents for potato breeding and propagation of: step (1) culturing the Pediococcus pentosaceus strains Weissella and WS3 YN12 by cfu of bacteria ratio of 1: 2 ratio were mixed to give co-cultured seed liquid, 10% of the inoculum, mixed after the seed was inoculated free of calcium...

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Abstract

The invention belongs to the field of agricultural cultivation, and particularly relates to a microbial agent for sweet potato seedling culture and rapid propagation and a preparation method and application thereof. Active ingredients of the microbial agent are pediococcus pentosaceus WS3 and Weissella YN12; the classification name of the pediococcus pentosaceus WS3 is Pediococcus pentosaceus, andthe preservation number of the pediococcus pentosaceus WS3 is CGMCC NO. 15345; and the classification name of the Weissella YN12 is Weissella sp., and the preservation number of the Weissella sp. isCGMCC NO.7.32. The compound microbial agent with good antibacterial effect and antibiotic sensitivity is selected, and the initial propagation speed of the microbial agent in sweet potatoes is increased, the acid production capacity is enhanced, and the adverse environment tolerance is improved by virtue of the assisting effect among strains, so that the activity of pathogenic bacteria in seed potatoes is effectively inhibited; and sweet potato seedlings are rapidly propagated, the quality and quantity of the sweet potato seedlings are further improved, and the raw material consumption of sweet potato seedling raising is reduced.

Description

technical field [0001] The invention belongs to the field of agricultural cultivation, and in particular relates to a microbial agent for sweet potato seedling cultivation and rapid propagation, a preparation method and application thereof. Background technique [0002] Sweet potato (Ipomoea batatas (L.), Sweet Potato) has the advantages of rich nutrition, good stability and high yield. It has the characteristics of food crops, economic crops, vegetable crops and forage crops. It is widely planted in more than 100 countries in the world. In this century, the sweet potato will become the fifth largest food energy supply plant in the world. Sweet potato is an important low-input, high-yield, drought-resistant, barren-resistant, multi-purpose food, feed, industrial raw material crop and new bioenergy crop in China. It ranks fourth in my country after rice, wheat and corn. . In 2012, the sweet potato planting area in Shandong was 245,000 hm 2 , accounting for 3.40% of the sown...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01H4/00A01N63/20A01P3/00A01P21/00C12R1/01
CPCA01H4/001A01H4/008A01N63/00C12N1/20
Inventor 刘全兰韩燕红刘璐付丽平王乾坤吕鑫陈旺
Owner QINGDAO UNIV OF SCI & TECH
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