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Microbial agent for sweet potato seedling culture and rapid propagation as well as preparation method and application thereof

A microbial inoculum and a technology for raising sweet potato seedlings are applied in the field of agricultural cultivation to achieve the effects of reducing the amount of raw materials used, improving the quality of sweet potato seedlings, and promoting growth.

Inactive Publication Date: 2020-11-06
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, probiotics have not been applied to sweet potato seedling cultivation and propagation. In view of the above-mentioned problems and characteristics of sweet potato planting, it is necessary to select lactic acid bacteria with good antibacterial effect and antibiotic sensitivity to prepare sweet potato seedling cultivation and rapid propagation microbial bacteria. agent to inhibit or kill potential or existing pathogenic bacteria in seed potatoes

Method used

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  • Microbial agent for sweet potato seedling culture and rapid propagation as well as preparation method and application thereof
  • Microbial agent for sweet potato seedling culture and rapid propagation as well as preparation method and application thereof
  • Microbial agent for sweet potato seedling culture and rapid propagation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Isolation and purification of bacterial strains

[0042] Collect the water near the Shengli Bridge in Qingdao and the commercially available goat milk in Haiqin Square, Sifang District, Qingdao, add it into sterile water at a ratio of 1:10 by volume, mix it well and perform serial dilution, and apply 100 μL of the bacterial solution of each dilution Clothed in MRS solid medium, anaerobic culture at 37°C for 48 hours; pick a single colony with a large transparent circle and streak 3 times on the MRS solid medium to obtain a pure single colony; pick a single colony without adding calcium carbonate In the MRS liquid culture medium, observe whether gas is produced, the gas-producing strain is heterofermentative lactic acid bacteria, and the non-gas-producing strain is homofermentative lactic acid bacteria; a total of 17 purified homozygous and heterofermentative lactic acid bacterial strains were isolated; these strains were inserted into MRS liquid Culture medium at 37...

Embodiment 2

[0067] Heme increases the proliferation density of Pediococcus pentosaceae WS3 and Weissella YN12.

[0068] Activation of lactic acid bacteria: Take out Pediococcus pentosaceae WS3 and Weissella YN12 stored at -20°C, inject 5 μL into MRS solid medium, spread, and culture at 37°C for 10-12 hours; Ring-pick a single colony on the MRS solid medium, inoculate it into a 10mL test tube containing 5mL of MRS liquid medium, and culture it at 37°C for 8-10 hours to obtain activated lactic acid bacteria. At this time, each ml of MRS liquid medium Contains at least 1×10 7 cfu lactic acid bacteria.

[0069] Co-cultivation of Pediococcus pentosaceae WS3 and Weissella YN12:

[0070] The two kinds of lactic acid bacteria liquids cultivated above are mixed according to the ratio of cfu of 1:2 to obtain the seed liquid for the co-cultivation of bacterial strains. According to the inoculum size of 10%, the mixed above-mentioned seed liquids are respectively inoculated in non-calcium carbonate...

Embodiment 3

[0072] Preparation and Application of Microbial Inoculum

[0073] (1) Activation of lactic acid bacteria: Take out the Pediococcus pentosaceae WS3 and Weissella YN12 obtained by screening in the above-mentioned Example 1, which are stored at -20°C, take 5 μL respectively and inject them on the MRS solid medium, spread them, and heat them at a temperature of 37 Cultivate at a temperature of 10-12 hours; use an inoculation loop to pick a single colony on the MRS solid medium, inoculate it into a 10mL test tube containing 5mL of MRS liquid medium, and cultivate it at a temperature of 37°C for 10 hours to obtain activated lactic acid bacteria. At this point, each ml of MRS liquid medium contains at least 1 × 10 7 cfu lactic acid bacteria.

[0074] (2) Preparation of microbial inoculum for sweet potato seedling cultivation and rapid propagation: the bacteria liquid of Pediococcus pentosaceae WS3 and Weissella YN12 cultivated in step (1) are mixed according to the cfu ratio of 1:2 ...

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Abstract

The invention belongs to the field of agricultural cultivation, and particularly relates to a microbial agent for sweet potato seedling culture and rapid propagation and a preparation method and application thereof. Active ingredients of the microbial agent are pediococcus pentosaceus WS3 and Weissella YN12; the classification name of the pediococcus pentosaceus WS3 is Pediococcus pentosaceus, andthe preservation number of the pediococcus pentosaceus WS3 is CGMCC NO. 15345; and the classification name of the Weissella YN12 is Weissella sp., and the preservation number of the Weissella sp. isCGMCC NO.7.32. The compound microbial agent with good antibacterial effect and antibiotic sensitivity is selected, and the initial propagation speed of the microbial agent in sweet potatoes is increased, the acid production capacity is enhanced, and the adverse environment tolerance is improved by virtue of the assisting effect among strains, so that the activity of pathogenic bacteria in seed potatoes is effectively inhibited; and sweet potato seedlings are rapidly propagated, the quality and quantity of the sweet potato seedlings are further improved, and the raw material consumption of sweet potato seedling raising is reduced.

Description

technical field [0001] The invention belongs to the field of agricultural cultivation, and in particular relates to a microbial agent for sweet potato seedling cultivation and rapid propagation, a preparation method and application thereof. Background technique [0002] Sweet potato (Ipomoea batatas (L.), Sweet Potato) has the advantages of rich nutrition, good stability and high yield. It has the characteristics of food crops, economic crops, vegetable crops and forage crops. It is widely planted in more than 100 countries in the world. In this century, the sweet potato will become the fifth largest food energy supply plant in the world. Sweet potato is an important low-input, high-yield, drought-resistant, barren-resistant, multi-purpose food, feed, industrial raw material crop and new bioenergy crop in China. It ranks fourth in my country after rice, wheat and corn. . In 2012, the sweet potato planting area in Shandong was 245,000 hm 2 , accounting for 3.40% of the sown...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01H4/00A01N63/20A01P3/00A01P21/00C12R1/01
CPCC12N1/20A01H4/001A01H4/008A01N63/00
Inventor 刘全兰韩燕红刘璐付丽平王乾坤吕鑫陈旺
Owner QINGDAO UNIV OF SCI & TECH
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