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A kind of dendrobium subculture propagation medium and preparation method thereof

A subculture propagation and culture medium technology, which is applied in the field of dendrobium subculture proliferation medium and its preparation, can solve the problems that the culture medium is not in the subculture proliferation stage, and there are few organic additives, so as to achieve good growth and short production cycle , The effect of strong tooth seedlings

Active Publication Date: 2022-03-29
广东生态工程职业学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, these mediums are not in the subculture propagation stage, and the organic additives added to the medium are relatively small, and they are all specially designed for Dendrobium candidum, and can only be used for tissue culture of Dendrobium candidum

Method used

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  • A kind of dendrobium subculture propagation medium and preparation method thereof
  • A kind of dendrobium subculture propagation medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of dendrobium subculture proliferation medium, comprises the raw material of following weight ratio:

[0036] Huabao No. 1 600mg / L; Huabao No. 2 600mg / L; ferrous sulfate 10mg / L; ethylenediaminetetraethylene disodium salt 20mg / L; glycine 0.9mg / L, naphthaleneacetic acid 0.1mg / L; sulfur hydrochloride Amine 0.05mg / L; pyridoxine hydrochloride 0.2mg / L; niacin 0.2mg / L; inositol 80mg / L; apple puree 6.5mg / L; banana puree 33mg / L; peptone 0.5mg / L; Protein 0.1mg / L; sucrose 10g / L and carrageenan 5g / L.

[0037] S1. Take Huabao No. 1, Huabao No. 2, ferrous sulfate and ethylenediaminetetraethylene disodium salt in the above weight ratio and stir and mix at a stirring speed of 400rpm / min for 20min to form a mixed solution A;

[0038] S2. Add glycine, naphthalene acetic acid, thiamine hydrochloride, pyridoxine hydrochloride, nicotinic acid, inositol, sucrose, carrageenan, hydrolyzed casein, apple puree, banana puree and peptone to the mixture A Stir and mix, the stirring speed i...

Embodiment 2

[0041] A kind of dendrobium subculture proliferation medium, comprises the raw material of following weight ratio:

[0042] Huabao No. 1 800mg / L; Huabao No. 2 1000mg / L; ferrous sulfate 20mg / L; ethylenediaminetetraethylene disodium salt 30mg / L; glycine 1.7mg / L, naphthaleneacetic acid 0.3mg / L; sulfur hydrochloride Amine 0.1mg / L; pyridoxine hydrochloride 0.5mg / L; niacin 0.4mg / L; inositol 90mg / L; apple puree 9mg / L; banana puree 40mg / L; mg / L; sucrose 20g / L and carrageenan 6g / L.

[0043] S1. Take Huabao No. 1, Huabao No. 2, ferrous sulfate and ethylenediaminetetraethylene disodium salt in the above weight ratio and stir and mix at a stirring speed of 500rpm / min for 30min to form a mixed solution A;

[0044] S2. Add glycine, naphthalene acetic acid, thiamine hydrochloride, pyridoxine hydrochloride, nicotinic acid, inositol, sucrose, carrageenan, hydrolyzed casein, apple puree, banana puree and peptone to the mixture A Stir and mix, the stirring speed is 1000rpm / min, and the stirring ...

Embodiment 3

[0047] A kind of dendrobium subculture proliferation medium, comprises the raw material of following weight ratio:

[0048] Huabao No. 1 1000mg / L; Huabao No. 2 1200mg / L; ferrous sulfate 30mg / L; ethylenediaminetetraethylene disodium salt 40mg / L; glycine 2.5mg / L, naphthaleneacetic acid 0.5mg / L; sulfur hydrochloride Amine 0.15mg / L; pyridoxine hydrochloride 0.8mg / L; niacin 0.7mg / L; inositol 100mg / L; apple puree 12mg / L; banana puree 56.5mg / L; peptone 1.5mg / L; Protein 0.5mg / L; sucrose 30g / L and carrageenan 8g / L.

[0049] S1. Take Huabao No. 1, Huabao No. 2, ferrous sulfate and ethylenediaminetetraethylene disodium salt in the above weight ratio and stir and mix at a stirring speed of 550rpm / min and a stirring time of 35min to form a mixed solution A;

[0050] S2. Add glycine, naphthalene acetic acid, thiamine hydrochloride, pyridoxine hydrochloride, nicotinic acid, inositol, sucrose, carrageenan, hydrolyzed casein, apple puree, banana puree and peptone to the mixture A Stir and mi...

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Abstract

The present invention relates to the field of biotechnology, in particular to a culture medium for the subculture of Dendrobium and a preparation method thereof. The culture medium for the subculture of Dendrobium comprises the following raw materials in weight ratio: Huabao No. 1; Huabao No. 2; ferrous sulfate ; edetine disodium salt; growth regulator; thiamine hydrochloride; pyridoxine hydrochloride; niacin; inositol; nutritional agent; hydrolyzed casein; sugars and coagulants. The culture medium of the present invention has a short seedling production cycle, a propagation speed of 5 to 7 times, a seedling rate of at least 95%, a 90-day transplant survival rate of at least 98%, and low cost. The cultivated tooth seedlings are strong, emerald green, neat, with a large number of roots, and the growth is better.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a dendrobium subculture proliferation medium and a preparation method thereof. Background technique [0002] Dendrobium is a plant in the family Orchidaceae, and common species include Dendrobium nobile, Dendrobium officinale, and Dendrobium fringe. Most varieties have the effects of nourishing the stomach and promoting body fluid, nourishing yin and clearing away heat. For example, Dendrobium candidum, known as the "Top Ten Immortal Herbs in China", is often used for fever, dry mouth and polydipsia, insufficient stomach yin, retching due to lack of food, persistent deficiency of heat after illness, hyperactivity of fire due to yin deficiency, etc. [0003] Orchidaceae are mostly epiphytic herbaceous plants, which grow on semi-shady rocks in high-altitude mountains, and are often associated with bryophytes, and they like warmth. Due to special growing conditions, limited distributi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 张华通谢腾飞赵静何旭君吴刚
Owner 广东生态工程职业学院