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Multifunctional nanozyme and its preparation method and application

A multi-functional, nano-enzyme technology, applied in chemical instruments and methods, catalyst activation/preparation, lanthanide oxides/hydroxides, etc., can solve the problems of expensive equipment, long measurement time, and easy inactivation of chromatography , to achieve the effect of overcoming easy inactivation, simple preparation method and high stability

Active Publication Date: 2022-03-08
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chromatography requires expensive equipment and takes a long time to measure, and the preparation of antibodies and enzymes required for immunological and enzymatic methods is costly and easily inactivated

Method used

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  • Multifunctional nanozyme and its preparation method and application
  • Multifunctional nanozyme and its preparation method and application
  • Multifunctional nanozyme and its preparation method and application

Examples

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preparation example Construction

[0028] The second technical problem to be solved by this invention is to provide the preparation method of above-mentioned multifunctional nanozyme, a kind of method that is easy to synthesize, and this method comprises the following steps:

[0029] The first step is to prepare CeO 2 : Mix a certain amount of cerium nitrate aqueous solution with the ethylene glycol solution of polyvinylpyrrolidone, under vigorous stirring, add excess sodium hydroxide solution to form a precipitate, then add appropriate amount of hydrogen peroxide solution, stir for 1 hour and then transfer to high temperature reaction kettle React at 120-350°C for 2-3 hours, cool to room temperature, collect the precipitate by centrifugation, wash the precipitate with ethanol for 3 times, and dry at 60-80°C to obtain CeO 2 .

[0030] The second step is to prepare multifunctional nanozymes: add CeO to a certain volume of aqueous solution containing terbium nitrate and copper nitrate 2 Ethanol solution, stir e...

Embodiment 1

[0035] Example 1 Multifunctional nanozyme CeO 2 Preparation of @CuTb

[0036] CeO 2 Preparation: 0.1 g Ce(NO 3 ) 3 ·6H 2 The cerium nitrate solution produced by dissolving O in 1 mL of water was mixed with the PVP ethylene glycol solution produced by dissolving 0.04 g of polyvinylpyrrolidone (PVP, molecular weight 40,000) in 6 mL of ethylene glycol, and 2 mL of 1 mM NaOH solution, add 1 mL of 30% H 2 o 2 The solution was stirred for 1 hour, then transferred to a high-temperature reactor for 2 hours at 160°C, cooled to room temperature (25°C), centrifuged to collect the precipitate, washed with ethanol for 3 times, and dried in an oven at 80°C for later use.

[0037] CeO 2 @CuTb preparation: To 5 mL DMF (N,N' dimethylformamide), sequentially add 1 mL 30 mM Tb (NO 3 ) 3 and 1 mL of 30 mM Cu (NO 3 ) 2 aqueous solution, and then add 2 mL containing 0.05 g CeO prepared above 2 Mixed solution 1 was generated by stirring evenly, and 1 mL of DMF solution containing 30 mM ...

Embodiment 2

[0038] Example 2 Multifunctional nanozyme CeO 2 Preparation of @CuTb

[0039] To 4 mL DMF, sequentially add 1 mL containing 30 mM Tb (NO 3 ) 3 and 1 mL containing 30 mM Cu (NO 3 ) 2 , then add 2 mL containing 0.05 g CeO prepared above 2 Mixed solution 1 was generated by stirring evenly, and 2 mL of DMF solution containing 30 mM dipicolinic acid was added to the mixed solution 1 to form terbium ion: copper ion: dipicolinic acid: CeO 2 The molar ratio of the mixed solution is 1:1:2:10. After continuous stirring for 10 minutes, the mixed solution is transferred to a high-temperature reactor, reacted at 140°C for 24 hours, cooled to room temperature, and centrifuged to collect the precipitate. After washing with pure water for 3 times, put it in a drying oven at 60°C and dry it for later use. figure 1 B is the prepared multifunctional nanozyme CeO 2 SEM images of @CuTb, the multifunctional nanozyme CeO 2 @CuTb is a spherical particle with an average particle size of 1.2 μm...

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Abstract

The invention discloses a multifunctional nanozyme, which is a nanomaterial formed by solvothermal reaction of terbium ions, copper ions, cerium oxide and pyridinedicarboxylic acid. The multifunctional nanozyme has the catalytic function and the luminescent indicating function of peroxidase and laccase, and can catalyze the degradation and luminescent display of diethylstilbestrol. The invention also discloses the preparation method of the multifunctional nanozyme and the application of degrading and measuring diethylstilbestrol. The multifunctional nanozyme of the invention has high catalytic activity and stability, low cost, and can be applied to the removal and monitoring of diethylstilbestrol in the environment.

Description

technical field [0001] The invention belongs to the technical fields of catalysis, environmental protection and luminescence detection, and relates to a multifunctional nanozyme and its preparation method and application. Background technique [0002] Diethylstilbestrol (DES) is a synthetic non-steroidal female hormone, which has the same pharmacological and therapeutic effects as natural estradiol, and its activity is stronger than estradiol. Medically, DES was used to treat estrogen deficiency in the early stage (promoting the growth and development of the uterus, fallopian tubes, breasts and protein synthesis, preventing miscarriage, etc.). In the 1970s, DES was found to increase the risk of various reproductive diseases (clear cell adenocarcinoma of the vagina and cervix, vaginal cancer, endometrial cancer, testicular abnormalities, etc.), and has certain teratogenic and carcinogenic effects and has been banned in many countries. However, diethylstilbestrol is still wid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J31/22B01J35/08B01J37/10C07F19/00C09K11/06C01F17/235G01N21/64C02F1/00C02F101/34
CPCB01J31/2243B01J35/08B01J35/0013B01J37/10C07F5/003C09K11/06C02F1/00C01F17/235G01N21/643C02F2101/345C02F2305/10C09K2211/182B01J2531/38B01J2531/16B01J2531/0213
Inventor 陈扬王莉
Owner SOUTHEAST UNIV
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