Method for improving triboelectric output performance of protein film by changing protein structure

[0032] In the control group, the vegetable protein film was prepared, and the vegetable protein film was prepared by the deposition method: first, the preparation of the film-forming solution, the insoluble protein powder was dissolved in an appropriate solvent: water or ethanol aqueous solution, and then an appropriate amount of glycerol was added as a plasticizer. After heat treatment, the protein is denatured to obtain a more extended structure required for film formation, and finally the solvent is evaporated to obtain a protein film with uniform texture, certain transparency and good flexibility.

[0033] The experimental group prepared the vegetable protein film by using the deposition method: first, the film-forming solution was prepared by co-dissolving the insoluble protein powder and a trace amount of another protein in a strong alkaline aqueous solution (1-5% , w/w), after a short period of time under these conditions, acidification to neutrality to allow charge redistribution to induce protein refolding, a process that will result in the burying of the hydrophobic groups of the protein and the exposure of the charged groups, thereby greatly reducing the The solubility of the protein is improved, and after removing salts and other small molecular compounds in the protein solution by dialysis, a homogeneous protein solution is formed under neutral conditions. After obtaining the protein solution, the same procedure was performed as the control group: adding an appropriate amount of glycerol as a plasticizer (reducing the interaction between protein chains to improve the tensile properties of the film, enhancing flexibility, and not easy to crack), and denaturing the protein after heat treatment to obtain The more extended structure required for film formation, and finally the solvent is evaporated to obtain a protein film with uniform texture, certain transparency and good flexibility.

[0034] After balancing the humidity, the protein membranes of the control group and the experimental group were used as the friction electron-donating layers respectively, and their output electrical properties were measured and compared.

[0035] Add a plasticizer (such as glycerol) with a mass of 20-50% (w/w) of the protein to the two groups of protein solutions, heat and stir in a water bath at 50-70°C for 30-60 minutes, and degas under vacuum for 10 minutes. Then pour it into a mold and put it in an oven to dry at 40-60°C. Finally, the dried film was peeled off, and the moisture was equilibrated under certain temperature and humidity conditions for subsequent electrical tests.

[0036] Implementation example

[0037] Example 1

[0038]The first is the preparation of the experimental histone solution. 1.25g of insoluble rice glutenin was dispersed in 25mL of aqueous solution (5%, w/w), and 0.0125g of another variety of rice glutenin was added. By adding 1M hydrogen Sodium oxide was adjusted to pH 12, so that it was fully dissolved in strong alkali. After stirring for 3 h, 1 M hydrochloric acid was added to acidify the solution to pH 10.5, and then 0.1 M hydrochloric acid was used to adjust the solution to pH 7.5. The entire pH cycle process needs to be carried out under stirring conditions. Finally, the salts and other small molecular compounds in the protein solution were removed by dialysis to obtain the film-forming solution of the experimental group.

[0039] like figure 1 As shown, compared with the negative control group (rice gluten without any treatment was fully hydrated in water), the absolute value of the potential of the experimental histone solution was higher, indicating that the experimental histone solution had better performance under neutral conditions. The storage stability provides the necessary conditions for the deposition method of insoluble rice glutenin under neutral conditions.