Method for preparing adenovirus vector vaccine through perfusion culture process

A culture method, adenovirus technology, applied in virus/bacteriophage, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of low cell density, high-density culture of 293 cells, limited growth, etc.
CN112094814AActive Publication Date: 2020-12-18CANSINO BIOLOGICS INC
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CANSINO BIOLOGICS INC
Publication Date
2020-12-18

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Abstract

The invention discloses a method for preparing an adenovirus vector vaccine through a perfusion culture process. The method comprises a step of culturing adenoviral host cells, in particular a step ofadjusting the perfusion rate depending on cell density (e.g. adjusting the perfusion rate for at least two stages). According to the method, high-density growth of the adenovirus host cells is realized, and meanwhile, the single-cell yield of infected viruses and the specific activity of a virus harvesting fluid are improved.
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Description

technical field

[0001] The invention relates to the technical field of biological products, in particular to a method for culturing an adenovirus host cell, a method for producing an adenovirus and a method for preparing an adenovirus vector vaccine, in particular to a method for preparing an adenovirus vector vaccine through a perfusion culture process. Background technique

[0002] Adenovirus is a non-enveloped DNA virus, which has the characteristics of easy infection, wide host range, low toxicity, safe use, large capacity, non-integration, low pathogenicity to humans, and no induction of cancer. Become one of the most promising gene carriers in gene transfer.

[0003] The modified adenovirus removes the E1 / E3 gene expression cassette, prevents the transcription of functional proteins dependent on the E1 region and the E3 region, and the subsequent replication of viral DNA and the production of viral coat proteins. The E1 / E3-deleted adenovirus can provide E1 / E3 region ...

Claims

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