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Direct organ generation type regeneration method of rhizoma acori graminei

A technology of organogenesis and regeneration, applied in the field of plant cell engineering, can solve the problems of low seed germination rate and germination rate, slow vegetative growth process, quality degradation of calamus, shorten the culture time, fast rooting speed, and overcome the cycle long effect

Inactive Publication Date: 2020-12-22
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, calamus is a perennial herb with a slow vegetative growth process, its seeds are extremely difficult to collect in the fruit, and the seed germination rate and germination rate are extremely low in the natural state
At present, Acorus calamus is mainly propagated through rhizomes. This propagation method is not only limited by seasons and materials, but also causes a large amount of viruses to accumulate in the plant due to long-term asexual reproduction, resulting in the degradation of the quality of Calamus calamus. It is difficult to obtain a large number of high-quality seedlings in a short period of time.

Method used

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  • Direct organ generation type regeneration method of rhizoma acori graminei
  • Direct organ generation type regeneration method of rhizoma acori graminei
  • Direct organ generation type regeneration method of rhizoma acori graminei

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1. Pretreatment and Disinfection of Explants

[0033] Choose a calamus that grows vigorously and is free from diseases and insect pests, and take young whip buds as explants. Rinse the explants with running water for 10 minutes, soak them in washing powder water for 10 minutes, then rinse them with running water for 30 minutes, soak them in alcohol with a mass fraction of 75% for 30 seconds in an ultra-clean workbench, rinse them with sterile water for 3 times, and place them again. 0.1% HgCl in 250mL mass fraction 2 Soak in the solution for 6min, in which HgCl 2 1mL Tween-80 needs to be added to the solution, and the HgCl2 Rinse with sterile water 4 times after disinfection, then blot excess water with sterile filter paper, and set aside.

[0034] 2. Primary culture

[0035] The sterilized whip buds were inoculated on the starting medium, one whip bud was inoculated in each bottle, and after 3 days of dark culture, they were cultured according to the culture conditi...

Embodiment 2

[0041] 1. Pretreatment and Disinfection of Explants

[0042] Select a calamus that grows vigorously and is free from diseases and insect pests, and cut its rhizome into 1-2 cm long stem segments with a single-sided blade as explants. Rinse the explants with running water for 10 minutes, soak them in washing powder water for 20 minutes, then rinse them with running water for 40 minutes, soak them in alcohol with a mass fraction of 75% for 60 seconds in an ultra-clean workbench, rinse them with sterile water for 3 times, and place them again. 0.1% HgCl in 250mL mass fraction 2 Soak in the solution for 8min, in which HgCl 2 1mL Tween-80 needs to be added to the solution, and the HgCl 2 Rinse with sterile water 4 times after disinfection, then blot excess water with sterile filter paper, and set aside.

[0043] 2. Primary culture

[0044] Inoculate the sterilized rhizomes on the starting medium, inoculate one stem section per bottle, and culture them in the dark for 5-7 days a...

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Abstract

The invention provides a direct organ generation type regeneration method of rhizoma acori graminei, and relates to the technical field of plant cell engineering. Rhizoma acori graminei whip buds or rhizomes are used as explants, complete plants are obtained through direct regeneration on the premise that callus is not conducted, propagation of rhizoma acori graminei is not limited by seasons andmaterials, and the defects of virus accumulation and germplasm degeneration in the rhizomes propagation process can be overcome; and the growth period is short, and the tissue culture seedling germination rate is high. The regeneration method overcomes the defects of high variation rate and long period of a callus regeneration path, and can also reduce the culture steps, shorten the culture time and improve the production efficiency of the rhizoma acori graminei tissue culture seedlings, so that the rooting speed is high, the roots are thick and strong, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of plant cell engineering, and in particular relates to a direct organogenesis regeneration method of calamus calamus. Background technique [0002] Iris (Acorus tatarinowii Schott) is a perennial herbaceous plant of the genus Iris in the family Araceae, also known as Acorus tatarinowii Schott, also known as Acorus tatarinowii Schott, and wild chives. The rhizomes are fragrant, the roots are fleshy, with many fibrous roots, the leaves are entire, arranged in two rows, the spadix (Buddha), the pedicels are green, and the spathe leaves are shaped. It likes a humid environment, not resistant to drought, and slightly cold-tolerant. It has an epiphytic habit, distributed in the southern part of the Yellow River Basin in China, and grows in wetlands at an altitude of 20-2000m, between stones on the bank or streams. [0003] According to "Chinese Pharmacopoeia (2020)", the rhizome of Acorus calamus is used as a m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 蔡永萍韩文龙章扬金青黄满畅王亮
Owner ANHUI AGRICULTURAL UNIVERSITY