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Urea detection kit as well as preparation method and use method thereof

A technology for detection kits and reagents, applied in biochemical equipment and methods, measuring devices, microbiological determination/inspection, etc., can solve the problems of complex components and unfavorable industrial production, and achieve simple components, high accuracy, and anti-interference powerful effect

Pending Publication Date: 2020-12-25
LANZHOU BAIYUAN GENE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defects that the components of the urea detection kit in the prior art are complex and unfavorable for industrial production, thereby providing a urea detection kit

Method used

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  • Urea detection kit as well as preparation method and use method thereof
  • Urea detection kit as well as preparation method and use method thereof
  • Urea detection kit as well as preparation method and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A urea detection kit, comprising R1 reagent and R2 reagent, wherein,

[0028] R1 reagent contains:

[0029]

[0030] Prepare raw materials according to the above ratio, dissolve tris (Tris) in 1L of purified water, stir until completely dissolved, then adjust the pH to 7.8, 0.105MPa / cm 2 High pressure treatment for 30 minutes to obtain Tris buffer;

[0031] Add adenosine diphosphate, urease and glutamate dehydrogenase into the Tris buffer and mix well to obtain R1 reagent.

[0032] R2 reagent contains:

[0033] Tris buffer 100mmol / L;

[0034] Reduced coenzyme Ⅰ 800U / L;

[0035] α-ketoglutarate 28mmol / L;

[0036] Prepare raw materials according to the above ratio, dissolve tris (Tris) in 1L of purified water, stir until completely dissolved, then adjust the pH to 7.8, 0.105MPa / cm 2 High pressure treatment for 30 minutes to obtain Tris buffer;

[0037] Add reduced coenzyme I and α-ketoglutarate to Tris buffer, and mix well to obtain R2 reagent.

[0038] Take th...

Embodiment 2

[0040] A urea detection kit, comprising R1 reagent and R2 reagent, wherein,

[0041] R1 reagent contains:

[0042]

[0043] Prepare raw materials according to the above ratio, dissolve tris (Tris) in 1L of purified water, stir until completely dissolved, then adjust the pH to 8, 0.105MPa / cm 2 High pressure treatment for 30 minutes to obtain Tris buffer;

[0044] Add adenosine diphosphate, urease and glutamate dehydrogenase into the Tris buffer and mix well to obtain R1 reagent.

[0045] R2 reagent contains:

[0046] Tris buffer 120mmol / L;

[0047] Reduced coenzyme Ⅰ 1000U / L;

[0048] α-ketoglutarate 24mmol / L;

[0049] Prepare raw materials according to the above ratio, dissolve tris (Tris) in 1L of purified water, stir until completely dissolved, then adjust the pH to 8, 0.105MPa / cm 2 High pressure treatment for 30 minutes to obtain Tris buffer;

[0050] Add reduced coenzyme I and α-ketoglutarate to Tris buffer, and mix well to obtain R2 reagent.

[0051] Take the R...

Embodiment 3

[0053] A urea detection kit, comprising R1 reagent and R2 reagent, wherein,

[0054] R1 reagent contains:

[0055]

[0056]

[0057] Prepare raw materials according to the above ratio, dissolve tris (Tris) in 1L of purified water, stir until completely dissolved, then adjust the pH to 8.2, 0.105MPa / cm 2 High pressure treatment for 30 minutes to obtain Tris buffer;

[0058]Add adenosine diphosphate, urease and glutamate dehydrogenase into the Tris buffer and mix well to obtain R1 reagent.

[0059] R2 reagent contains:

[0060] Tris buffer 140mmol / L;

[0061] Reduced coenzyme Ⅰ 1200U / L;

[0062] α-ketoglutarate 20mmol / L;

[0063] Prepare raw materials according to the above ratio, dissolve tris (Tris) in 1L of purified water, stir until completely dissolved, then adjust the pH to 8.2, 0.105MPa / cm 2 High pressure treatment for 30 minutes to obtain Tris buffer;

[0064] Add reduced coenzyme I and α-ketoglutarate to Tris buffer, and mix well to obtain R2 reagent.

[0...

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Abstract

The invention provides a urea detection kit as well as a preparation method and a use method thereof. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a Tris buffer solution, adenosine diphosphate, urease and glutamate dehydrogenase, and the reagent R2 comprises a Tris buffer solution, reduced coenzyme I and alpha-ketoglutaric acid. The kit provided by the invention is simple in component, maintains the advantages of higher sensitivity, wide linear range, low cost, strong anti-interference capability and high accuracy by adopting an enzyme coupling rate methodin an enzyme method, and can be universally applied to full-automatic and semi-automatic analyzers.

Description

technical field [0001] The invention relates to the field of biological preparations, in particular to a urea detection kit, a preparation method and a use method thereof. Background technique [0002] Urea is the main end product of protein metabolism in the body. It has a small molecular weight and does not bind to plasma proteins. It can be freely filtered by the glomerulus. About 50% of the urea entering the primary urine is reabsorbed by the renal tubules and collecting ducts, and a small amount is excreted by the renal tubules. When the renal parenchyma is damaged, the glomerular filtration rate decreases and the blood urea concentration increases. The glomerular filtration function can be observed by measuring the blood urea or blood urea nitrogen concentration. [0003] At present, the determination methods of blood urea can be generally classified into enzymatic methods and chemical methods. enzymatic method. The amount of ammonium ions generated during the react...

Claims

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Application Information

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IPC IPC(8): C12Q1/58C12Q1/32
CPCC12Q1/58C12Q1/32G01N2333/90616
Inventor 车团结李春李琳张莹冯海霞常运朝李潇玲
Owner LANZHOU BAIYUAN GENE TECH
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